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Antitumour efficacy of Piper tuberculatum and piplartine based on the hollow fiber assay
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Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal do Piauí. Departamento de Biofísica e Fisiologia. Programa de Pós-Graduação em Ciências Farmacêuticas. Teresina, PI, BrasilUniversidade de São Paulo. Faculdade de Medicina. Departamento de Radiologia. São Paulo, SP, BrasilUniversidade Federal do Ceará. Departamento de Química Orgânica. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Departamento de Química Orgânica. Fortaleza, CE, BrasilUniversidade de São Paulo. Faculdade de Medicina. Departamento de Radiologia. São Paulo, SP, BrasilUniversidade Federal do Ceará. Faculdade de Medicina. Departamento de Fisiologia e Farmacologia. Fortaleza, CE, Brasil / Fundação Oswaldo Cruz. Fortaleza, CE, BrasilPiper tuberculatum, popularly known in Brazil as
“jaborandi falso” and “pimenta darta”, is widely
used in folk medicine for the treatment of several
diseases. In this study, the in vivo hollow fiber assay
was used to investigate the antitumour efficacy
of the crude extract and piplartine obtained
from P. tuberculatum roots. Human glioblastoma
(SF-295) and colon carcinoma (HCT-8) cell lines
were used. In vitro cytotoxicity was assayed by
the MTT assay. In the hollow fiber assay, nude
mice implantedwith tumour cells in hollow fibers
were treated for four consecutive days via the intraperitoneal
route, and tumour cell populations
were assessed by the MTT assay. Both the crude
extract and piplartine displayed cytotoxicity. In
the hollow fiber assay, tumour growth inhibition
rates were 24.6–54.8% for the crude extract and
33.7–62.2% for piplartine. No signal of toxicity
was noticed. In conclusion, the crude extract and
piplartine obtained from P. tuberculatum roots
displayed in vitro and in vivo anticancer efficacy