Automation of ChIP-Seq Library Preparation for Next Generation Sequencing on the ep Motion

ChIP-Seq library preparation can be a challenging procedure to automate because of its low ChIP DNA input. This Application Note describes the successful automation of Illumina ChIP-Seq library preparation on the Eppendorf epMotion 5075 TMX, using the KAPA High-Throughput Library Preparation Kit from Kapa Biosystems. Size-selected libraries were prepared from as little as 1 ng of fragmented ChIP DNA. To obtain the recommended 100 ng of library material for sequencing on the Illumina Genome AnalyzerIIx, only 18 amplification cycles were needed for 1 ng of input DNA, or 9 cycles when starting library construction with 10 ng of ChIP DNA

Co‐designed substrate‐integrated waveguide filters with patch antennas

A new approach to integrate high-quality (Q)-factor three-dimensional vertical cavity filters with patch antennas into a single unit is presented. This integrated filter/antenna system exhibits small footprint, high efficiency and enhanced bandwidth. The patch antenna is designed to provide an additional transmission pole for the filtering function and works as a highly efficient radiator. In addition, this integrated patch antenna witha filter can achieve a bandwidth larger than that of a standalone patch antenna with the same geometry. A prototype three-pole Chebyshev filter/antenna is demonstrated at X band.The centre frequency and fractional bandwidth of the filter/antenna system are 10.27 GHz and 8.7%, respectively. Owing to the high Q factor ( ∼650) of the cavity resonator and near loss-less transition between the antenna and filter, the overall efficiency of the filter/antenna system is simulated to be as high as 91.8%. The reduced footprint, enhanced bandwidth and low-loss performance of these integrated filter/antenna systems make them particularly useful for phased array applications. Prototypes of the filter/antenna systemsas well as a filter/antenna array are fabricated and measured. An excellent agreement between the simulated and measured results is observed. © The Institution of Engineering and Technology 2013