Small Interfering RNA Targeting M2 Gene Induces Effective and Long Term Inhibition of Influenza A Virus Replication

RNA interference (RNAi) provides a powerful new means to inhibit viral infection specifically. However, the selection of siRNA-resistant viruses is a major concern in the use of RNAi as antiviral therapeutics. In this study, we conducted a lentiviral vector with a H1-short hairpin RNA (shRNA) expression cassette to deliver small interfering RNAs (siRNAs) into mammalian cells. Using this vector that also expresses enhanced green fluorescence protein (EGFP) as surrogate marker, stable shRNA-expressing cell lines were successfully established and the inhibition efficiencies of rationally designed siRNAs targeting to conserved regions of influenza A virus genome were assessed. The results showed that a siRNA targeting influenza M2 gene (siM2) potently inhibited viral replication. The siM2 was not only effective for H1N1 virus but also for highly pathogenic avian influenza virus H5N1. In addition to its M2 inhibition, the siM2 also inhibited NP mRNA accumulation and protein expression. A long term inhibition effect of the siM2 was demonstrated and the emergence of siRNA-resistant mutants in influenza quasispecies was not observed. Taken together, our study suggested that M2 gene might be an optimal RNAi target for antiviral therapy. These findings provide useful information for the development of RNAi-based prophylaxis and therapy for human influenza virus infection

Design of a Novel Terahertz Metamaterial Absorber for Sensing Applications

This paper presents and evaluates a new terahertz metamaterial absorber (MMA) for sensing applications. Because of its unique properties, metamaterial-based sensors are widely employed in a variety of applications. The reported structure comprises of two identical metallic patches, a dielectric spacer and a ground metal plane. The finite element approach has been utilized to simulate and analyse the design. It is found that the MMA offered a prominent resonant peak with near 100% absorbance at frequency 4.5 THz due to the resultant effect of coupling between the two identical patches. In addition, surface current distribution, absorption mechanism and structural parametric analysis has also been investigated. The peak is designated as \u27A\u27, with a line width of 0.02 THz and a quality factor (Q-factor) of 225, which is sensitive to the refractive index of the environment (RI). As a result of its highly sensitive sensing capabilities, the proposed design can be employed as a sensor for refractive index, having 1.6 THz per refractive index unit (RIU) sensitivity and figure of merit (FoM) of 80 in terms of change in RI of the environment. The majority of biomedical samples have RI of 1.3 to 1.36, which is worth highlighting. Thus, biomedical applications may be possible with the suggested sensor