qRT-PCR of infestin.

<p>Adult insects infected with <i>T. cruzi</i> and uninfected <i>T. infestans</i> were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired <i>t</i> test was performed for statistical analysis.</p

A pie chart showing the species distribution of BLASTx hits of the <i>T. infestans</i> clusters for several organisms.

<p>A pie chart showing the species distribution of BLASTx hits of the <i>T. infestans</i> clusters for several organisms.</p

A list of contigs with unchanged expression that are important for metabolic processes or defense in <i>T. infestans</i>.

<p>FABL – Fatty acid binding lipocalin.</p><p>JHBP – Juvenile hormone binding protein.</p

Length distribution of <i>T. infestans</i> clusters.

<p>Length distribution of <i>T. infestans</i> clusters.</p

Functional classification of transcripts from all clusters.

<p>Functional classification of transcripts from all clusters.</p

Functional classification of transcripts that originated from upregulated contigs.

<p>Functional classification of transcripts that originated from upregulated contigs.</p

qRT-PCR of Thioredoxin reductase upon <i>T. cruzi</i> infection.

<p>The levels of mRNA from Thioredoxin reductase were obtained by relative quantification. Adult insects infected with <i>T. cruzi</i> and uninfected <i>T. infestans</i> were used for analysis (three biological samples were used for both the uninfected and infected groups). All data were normalized to 18S ribosomal RNA, representing the mean (n = 3) of identical triplicates ± standard deviation. An unpaired <i>t</i> test was performed for statistical analysis, and differences were considered significant at P<0.05. Asterisks represent significant differences (*** P<0.001).</p

Volume proportion of collagen and elastic fibers are shown in the first (A) and second protocols (B).

<p>A) * p = 0.031 compared to the other groups; ** p≤0.001 compared to S groups; B) * p = 0.035 compared to the other groups; ** p = 0.0003 compared to S groups. Values are means and SD.</p