Nanodelivery of a functional membrane receptor to manipulate cellular phenotype.

Modification of membrane receptor makeup is one of the most efficient ways to control input-output signals but is usually achieved by expressing DNA or RNA-encoded proteins or by using other genome-editing methods, which can be technically challenging and produce unwanted side effects. Here we develop and validate a nanodelivery approach to transfer in vitro synthesized, functional membrane receptors into the plasma membrane of living cells. Using β2-adrenergic receptor (β2AR), a prototypical G-protein coupled receptor, as an example, we demonstrated efficient incorporation of a full-length β2AR into a variety of mammalian cells, which imparts pharmacologic control over cellular signaling and affects cellular phenotype in an ex-vivo wound-healing model. Our approach for nanodelivery of functional membrane receptors expands the current toolkit for DNA and RNA-free manipulation of cellular function. We expect this approach to be readily applicable to the synthesis and nanodelivery of other types of GPCRs and membrane receptors, opening new doors for therapeutic development at the intersection between synthetic biology and nanomedicine

Sim-to-Real Segmentation in Robot-assisted Transoral Tracheal Intubation

Robotic-assisted tracheal intubation requires the robot to distinguish anatomical features like an experienced physician using deep-learning techniques. However, real datasets of oropharyngeal organs are limited due to patient privacy issues, making it challenging to train deep-learning models for accurate image segmentation. We hereby consider generating a new data modality through a virtual environment to assist the training process. Specifically, this work introduces a virtual dataset generated by the Simulation Open Framework Architecture (SOFA) framework to overcome the limited availability of actual endoscopic images. We also propose a domain adaptive Sim-to-Real method for oropharyngeal organ image segmentation, which employs an image blending strategy called IoU-Ranking Blend (IRB) and style-transfer techniques to address discrepancies between datasets. Experimental results demonstrate the superior performance of the proposed approach with domain adaptive models, improving segmentation accuracy and training stability. In the practical application, the trained segmentation model holds great promise for robot-assisted intubation surgery and intelligent surgical navigation.Comment: Extended abstract in IEEE ICRA 2023 Workshop (New Evolutions in Surgical Robotics: Embracing Multimodal Imaging Guidance, Intelligence, and Bio-inspired Mechanisms

Fabrication of CuOx thin-film photocathodes by magnetron reactive sputtering for photoelectrochemical water reduction

The CuOx thin film photocathodes were deposited on F-doped SnO2 (FTO) transparent conducting glasses by alternating current (AC) magnetron reactive sputtering under different Ar:O2 ratios. The advantage of this deposited method is that it can deposit a CuOx thin film uniformly and rapidly with large scale. From the photoelectrochemical (PEC) properties of these CuOx photocathodes, it can be found that the CuOx photocathode with Ar/O2 30:7 provide a photocurrent density of −3.2 mA cm−2 under a bias potential −0.5 V (vs. Ag/AgCl), which was found to be twice higher than that of Ar/O2 with 30:5. A detailed characterization on the structure, morphology and electrochemical properties of these CuOx thin film photocathodes was carried out, and it is found that the improved PEC performance of CuOx semiconductor photocathode with Ar/O2 30:7 attributed to the less defects in it, indicating that this Ar/O2 30:7 is an optimized condition for excellent CuOx semiconductor photocathode fabrication

Domain Adaptive Sim-to-Real Segmentation of Oropharyngeal Organs

Video-assisted transoral tracheal intubation (TI) necessitates using an endoscope that helps the physician insert a tracheal tube into the glottis instead of the esophagus. The growing trend of robotic-assisted TI would require a medical robot to distinguish anatomical features like an experienced physician which can be imitated by utilizing supervised deep-learning techniques. However, the real datasets of oropharyngeal organs are often inaccessible due to limited open-source data and patient privacy. In this work, we propose a domain adaptive Sim-to-Real framework called IoU-Ranking Blend-ArtFlow (IRB-AF) for image segmentation of oropharyngeal organs. The framework includes an image blending strategy called IoU-Ranking Blend (IRB) and style-transfer method ArtFlow. Here, IRB alleviates the problem of poor segmentation performance caused by significant datasets domain differences; while ArtFlow is introduced to reduce the discrepancies between datasets further. A virtual oropharynx image dataset generated by the SOFA framework is used as the learning subject for semantic segmentation to deal with the limited availability of actual endoscopic images. We adapted IRB-AF with the state-of-the-art domain adaptive segmentation models. The results demonstrate the superior performance of our approach in further improving the segmentation accuracy and training stability.Comment: The manuscript is accepted by Medical & Biological Engineering & Computing. Code and dataset: https://github.com/gkw0010/EISOST-Sim2Real-Dataset-Releas

Hydraulic fracturing propagation mechanism during shale gas reservoir stimulation through horizontal well

Način razlamanja stijenske mase u naslagama naftnih škriljaca jedan je od glavnih čimbenika koji djeluju na učinkovitost hidrauličkog frakturiranja (frakiranja). U ovom su radu provedena fizikalna ispitivanja i numeričko modeliranje u svrhu sustavnog ispitivanja učinka in-situ (podzemnog) naprezanja i kuta bušenja na stvaranje tlaka zbog hidrauličkog frakturiranja, širenje razlamanja i način razlamanja u horizontalnoj bušotini naftnog nalazišta Shengli u Luojia pokrajini, u izvođenju Sinopec Corp. Ukupno je razmatrano šest različitih in-situ kombinacija naprezanja i osam različitih kutova bušenja slojevite stijenske mase tijekom hidrauličkog frakturiranja. Sažetak nastanka i širenja pukotine te završni oblici pukotina nastalih hidrauličkim frakturiranjem u slojevitim stijenskim masama otkrivaju da kod stratificiranih stijena s istim kutom bušenja, što je veći in-situ omjer naprezanja (t.j. niže maksimalno horizontalno osnovno naprezanje pri konstantnim vertikalnim naprezanjem), potreban je niži hidraulički tlak za poticanje i širenje hidrauličkog frakturiranja. Štoviše, ustanovljeno je da je kod stratificirane stijenske mase pri istom omjeru naprezanja, tlak hidrauličkog frakturiranja, u slučaju kad je kut bušenja 30°, veći nego u svim drugim slučajevima. Nadalje, zapaženo je da učinak stratifikacije na hidrauličko frakturiranje postaje slabiji s porastom in-situ omjera naprezanja. Konačno je zaključeno da rezultati ove analize mogu biti važan teorijski pokazatelj u poboljšanju oblikovanja hidrauličkog frakturiranja kako bi se osiguralo učinkovito stimuliranje naslaga naftnih škriljaca.The fracture pattern of rock mass in shale gas reservoirs is one of the main factors affecting the efficiency of hydraulic fracturing. In this paper, physical experiments and numerical modelling were conducted to systematically investigate the effect of the in-situ stress and perforation angle on the hydraulic fracture initiation pressure and location, fracture propagation, and fracture pattern in a horizontal well drilled by Sinopec Corp. in Luojia area of Shengli Oilfield. A total of six different in-situ stress combinations and eight different perforation angles were considered for the stratified rock mass during the hydraulic fracturing. A summary of the fracture initiations and propagation, and the final fracture patterns induced by the hydraulic fracturing in the stratified rock masses reveals that, for the stratified rock masses with the same perforation angle, the larger the in-situ stress ratio (i.e. lower maximum horizontal principal stress when the vertical stress remains constant) is, the lower hydraulic pressure is required for hydraulic fracturing initiation and propagation. Moreover, it is found that, for the stratified rock mass under the same stress ratio, the hydraulic fracturing pressure in the case with a perforation angle of 30° is higher than that in all other cases. Furthermore, it is noted that the effect of the stratification on the hydraulic fracturing becomes weaker with the in-situ stress ratio increasing. It is finally concluded that the results from this study can provide important theoretical guidance for improving the hydraulic fracturing design in order to ensure the effective shale gas reservoir stimulations

Graphene quantum dot modified g-C3N4 for enhanced photocatalytic oxidation of ammonia performance

In this study, graphene quantum dot (GQD) modified g-C3N4 (GQDs/CN) composite photocatalysts were prepared. The photocatalytic ammonia degradation properties of the GQDs/CN composites were much higher than that of pure g-C3N4. When the amount of GQDs added reached 0.5 wt% the GQDs/CN composite showed the best performance for photocatalytic total ammonia nitrogen (TAN) removing, and a 90% TAN removing rate was achieved in 7 hours under visible light illumination (200 mW cm−2), which is approximately 3 times higher than that of pure g-C3N4. The increased photocatalytic property was contributed by the photon adsorption ability and electron transfer capacity, which were improved after GQD modification. The main photocatalytic end-product of TAN was NO3− which is a type of environmentally green ion. Further results indicated that the oxygen concentration and pH value of the reaction solution were very important for the photocatalytic ammonia degradation process. A better performance could be achieved under a higher oxygen concentration and pH value

Comprehensive Evaluation of Self-Healing Polyampholyte Gel Particles for the Severe Leakoff Control of Drilling Fluids

Lost circulation has been a serious problem to be solved in many drilling practices during oil, gas and geothermal well drillings. Many materials have been developed and evaluated for the purpose. However, their performance to plug severe leakoff is very limited. Herein, an injectable self-healing hydrogel based on polyampholyte with sulfonated and quaternary ammonium functionalities (P(MPTC-co-NaSS)) was developed and comprehensively evaluated to prevent the severe loss of fluids to formation. By incorporating cation-π (π is for aromatic residues) interaction, the hydrogel shown self-healing property and robustness in severe environment (temperature, salt) by comparison with other hydrogels merely consisting of cation-anion and H-bonding interactions. Aromatic residues enhanced thermal stability above 310 °C. The plugging measurement shown that an addition of 2 wt% dried gel particles can plug high-permeability formation and endure a high pressure of 6 MPa, produce much lower circulation loss and result in a dramatically increased loss volume reduction rate (63.5%) compared with a commercial polymer gel product and an inert material (9.4%) after a self-healing process. Markedly, P(MPTC-co-NaSS) can be used in a wide range of formation temperature (as high as 150 °C) and salt concentrations (NaCl, CaCl2, as high as 15 wt %). In addition to suitable particle size and mechanically robustness, it was also attributed to the soft, swelling, deformable, toughness and self-healable features of P(MPTC-co-NaSS) gel particles as well as the strong adhesion to negatively charged formations in water, even under high thermal and saline condition. These characteristics also contributed to a long-term plugging performance, beneficial to avoid repeated lost circulation in drilling operation. Besides, this self-healing polyampholyte gel particles dispersed well in saline fluid and maintained stable rheological properties after hot rolling, which was favorable to drilling fluid circulation. This study shown the application potential of self-healing materials as plugging material candidate in petroleum drilling industry

Nitrogen-doped carbon nanospheres-modified graphitic carbon nitride with outstanding photocatalytic activity

Metals and metal oxides are widely used as photo/electro-catalysts for environmental remediation. However, there are many issues related to these metal-based catalysts for practical applications, such as high cost and detrimental environmental impact due to metal leaching. Carbon-based catalysts have the potential to overcome these limitations. In this study, monodisperse nitrogen-doped carbon nanospheres (NCs) were synthesized and loaded onto graphitic carbon nitride (g-C3N4, GCN) via a facile hydrothermal method for photocatalytic removal of sulfachloropyridazine (SCP). The prepared metal-free GCN-NC exhibited remarkably enhanced efficiency in SCP degradation. The nitrogen content in NC critically influences the physicochemical properties and performances of the resultant hybrids. The optimum nitrogen doping concentration was identified at 6.0 wt%. The SCP removal rates can be improved by a factor of 4.7 and 3.2, under UV and visible lights, by the GCN-NC composite due to the enhanced charge mobility and visible light harvesting. The mechanism of the improved photocatalytic performance and band structure alternation were further investigated by density functional theory (DFT) calculations. The DFT results confirm the high capability of the GCN-NC hybrids to activate the electron–hole pairs by reducing the band gap energy and efficiently separating electron/hole pairs. Superoxide and hydroxyl radicals are subsequently produced, leading to the efficient SCP removal