Perinatal stem cells revisited: directions and indications at the crossroads between tissue regeneration and repair.

Perinatal stem cells research attracted great interest worldwide in recent years. Foetus-associated tissues contain various populations of stem cells, most of which are comprised within the category of mesenchymal stem cells (MSCs). This special issue collects both reviews and original reports on all the perinatal stem cell types which are currently under investigation. These cells have multiple promising features: differentiative capacity towards mature cell types of all the three germ layers, hypoimmunogenicity in vitro and in vivo, ease of sourcing, ex vivo culture and stor- age. In particular, immune modulation is viewed as a prom- ising feature of many MSCs populations, since these cells, once administered therapeutically, may be able to overcome, or at least evade, the host immune response which may lead to acute or chronic rejection of the transplant

Ten kilodalton heat shock protein (HSP10) is overexpressed during carcinogenesis of large bowel and uterine exocervix

In the present study, we evaluated the presence and the level of expression of HSP10 in two carcinogenetic models: the 'adenoma-carcinoma sequence' of large bowel and the 'dysplasia-carcinoma sequence' of uterine exocervix. We found HSP10 was overexpressed during the carcinogenesis of both organs. In particular, HSP10 was overexpressed early in large bowel carcinogenesis, while the expression of this protein in exocervical carcinogenesis gradually increased from normal through dysplastic to neoplastic tissues. The quantitative analysis of immunohistochemistry and the Western blotting confirmed these results. Our previous observations showed overexpression of HSP60 in the same carcinogenetic models. This report correlates the overexpression of HSP10 with that of HSP60 during carcinogenesis in vivo. These results could stimulate further studies on the pathogenetic role of these proteins during the carcinogenesis as well as their use as diagnostic and prognostic tools in oncology. \ua9 2003 Elsevier Science Ltd. All rights reserved

L’ambiente marino costiero: aspetti e tutela. Progetto formativo di Alternanza Scuola Lavoro 2016‐2019 Liceo Scienze Applicate

Il percorso formativo consente di acquisire nozioni teoriche e pratiche sulle moderne tecniche (strumentali e metodologiche) di investigazione del “datum” geologico finalizzate allo studio multidisciplinare dell’ambiente marino‐costiero, con particolare riguardo alle ricerche sperimentali che l’Istituto IAMC ha condotto e conduce nel Golfo di Napoli. Vengono trattate anche tematiche di gestione del sistema sicurezza e qualità con particolare riguardo alle attività lavorative di ricerca (acquisizione, elaborazione e restituzione del dato),nonché indicazioni di procedure gestionali di progetto finalizzate al corretto utilizzo della risorsa umana e strumentale

Publisher's Note: Defect Influence on Heteroepitaxial 3C-SiC Young's Modulus [Electrochem. Solid-State Lett., 14, H161 (2011)]

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Conveying Audience Emotions through Humanoid Robot Gestures to an Orchestra during a Live Musical Exhibition

In the last twenty years, robotics have been applied in many heterogeneous contexts. Among them, the use of humanoid robots during musical concerts have been proposed and investigated by many authors. In this paper, we propose a contribution in the area of robotics application in music, consisting of a system for conveying audience emotions during a live musical exhibition, by means of a humanoid robot. In particular, we provide all spectators with a mobile app, by means of which they can select a specific color while listening to a piece of music (act). Each color is mapped to an emotion, and the audience preferences are then processed in order to select the next act to be played. This decision, based on the overall emotion felt by the audience, is then communicated by the robot through body gestures to the orchestra. Our first results show that spectators enjoy such kind of interactive musical performance, and are encouraging for further investigations

Determination of the photodisintegration reaction rates involving charged particles: systematical calculations and proposed measurements based on Extreme Light Infrastructure - Nuclear Physics (ELI-NP)

Photodisintegration reaction rates involving charged particles are of relevance to the p-process nucleosynthesis that aims at explaining the production of the stable neutron-deficient nuclides heavier than iron. In this study, the cross sections and astrophysical rates of (g,p) and (g,a) reactions for about 3000 target nuclei with 10<Z<100 ranging from stable to proton dripline nuclei are computed. To study the sensitivity of the calculations to the optical model potentials (OMPs), both the phenomenological Woods-Saxon and the microscopic folding OMPs are taken into account. The systematic comparisons show that the reaction rates, especially for the (g,a) reaction, are dramatically influenced by the OMPs. Thus the better determination of the OMP is crucial to reduce the uncertainties of the photodisintegration reaction rates involving charged particles. Meanwhile, a gamma-beam facility at ELI-NP is being developed, which will open new opportunities to experimentally study the photodisintegration reactions of astrophysics interest. Considering both the important reactions identified by the nucleosynthesis studies and the purpose of complementing the experimental results for the reactions involving p-nuclei, the measurements of six (g,p) and eight (g,a) reactions based on the gamma-beam facility at ELI-NP and the ELISSA detector for the charged particles detection are proposed, and the GEANT4 simulations are correspondingly performed. The minimum required energies of the gamma-beam to measure these reactions are estimated. It is shown that the direct measurements of these photonuclear reactions within the Gamow windows at T_9=2.5 for p-process are fairly feasible and promising at ELI-NP. The expected experimental results will be used to constrain the OMPs of the charged particles, which can eventually reduce the uncertainties of the reaction rates for the p-process nucleosynthesis.Comment: 14 pages, 8 figures, Phys. Rev. C accepte

Role of chronic exsposure to cigarette smoke on endoglin/CD105 expression in airway epithelium.

Dysregulation of airway epithelial cell function related to cigarette smoke exposure plays an important role in the pathophysiology of COPD and is associated to tissue damage and disease severity. CD105 is a component of the receptor complex of TGF-β, a pleiotropic cytokine involved in cellular proliferation, differentiation and migration. CD105 regulates the expression of different components of the extracellular matrix suggesting a role of CD105 in cellular transmigration and remodeling processes. The aim of the present study was to investigate the expression of endoglin/CD105 in airway epithelium of COPD patients and its involvement in tissue remodeling and progression of COPD. We evaluated the immunoreactivity for CD105 expression in bronchial biopsies isolated from COPD patients and healthy controls (HC). The analysis of metaplastic epithelium was performed in bronchial biopsies by Image Analysis software (Leica Quantimet system). Finally, we investigated the expression of CD105 protein receptor in human bronchial epithelial cells (16HBE cells) exposed to 5% Cigarette Smoke Extract (CSE) for 12 days by western blot. We found that the CD105 immunoreactivity was significantly higher in bronchial epithelium of COPD than HC. Morphometric analysis of bioptic samples of COPD showed an increase of the immunoreactivity for CD105 in the area of metaplastic than in not metaplastic epithelium. Long term exposure to CSE significantly up-regulated CD105 expression in 16HBE. Chronic inflammation due to cigarette smoke might play a critic role on the alteration of CD105 protein expression in COPD, promoting tissue remodeling, angiogenesis and dysregulation of physiological reparative mechanisms, leading to squamous metaplasia

Probiogenomics analysis of 97 lactobacillus crispatus strains as a tool for the identification of promising next-generation probiotics

Members of the genus Lactobacillus represent the most common colonizers of the human vagina and are well-known for preserving vaginal health and contrasting the colonization of oppor-tunistic pathogens. Remarkably, high abundance of Lactobacillus crispatus in the vaginal environment has been linked to vaginal health, leading to the widespread use of many L. crispatus strains as probi-otics. Nevertheless, despite the scientific and industrial relevance of this species, a comprehensive investigation of the genomics of L. crispatus taxon is still missing. For this reason, we have performed a comparative genomics analysis of 97 L. crispatus strains, encompassing 16 strains sequenced in the framework of this study alongside 81 additional publicly available genome sequences. Thus, allowing the dissection of the L. crispatus pan-genome and core-genome followed by a comprehensive phylogenetic analysis based on the predicted core genes that revealed clustering based on ecological origin. Subsequently, a genomics-targeted approach, i.e., probiogenomics analysis, was applied for in-depth analysis of the eight L. crispatus strains of human origin sequenced in this study. In detail their genetic repertoire was screened for strain-specific genes responsible for phenotypic features that may guide the identification of optimal candidates for next-generation probiotics. The latter includes bacteriocin production, carbohydrates transport and metabolism, as well as a range of features that may be responsible for improved ecological fitness. In silico results regarding the genetic repertoire involved in carbohydrate metabolism were also validated by growth assays on a range of sugars, leading to the selection of putative novel probiotic strains

Wharton’s Jelly Mesenchymal Stromal Cells Support the Expansion of Cord Blood–derived CD34 + Cells Mimicking a Hematopoietic Niche in a Direct Cell–cell Contact Culture System

Wharton’s jelly mesenchymal stromal cells (WJ-MSCs) have been recently exploited as a feeder layer in coculture systems to expand umbilical cord blood–hematopoietic stem/progenitor cells (UCB-HSPCs). Here, we investigated the role of WJ-MSCs in supporting ex vivo UCB-HSPC expansion either when cultured in direct contact (DC) with WJ-MSCs or separated by a transwell system or in the presence of WJ-MSC–conditioned medium. We found, in short-term culture, a greater degree of expansion of UCB-CD34 + cells in a DC system (15.7 ± 4.1-fold increase) with respect to the other conditions. Moreover, in DC, we evidenced two different CD34 + cell populations (one floating and one adherent to WJ-MSCs) with different phenotypic and functional characteristics. Both multipotent CD34 + /CD38 − and lineage-committed CD34 + /CD38 + hematopoietic progenitors were expanded in a DC system. The former were significantly more represented in the adherent cell fraction than in the floating one (18.7 ± 11.2% vs. 9.7 ± 7.9% over the total CD34 + cells). Short-term colony forming unit (CFU) assays showed that HSPCs adherent to the stromal layer were able to generate a higher frequency of immature colonies (CFU-granulocyte/macrophage and burst-forming unit erythroid/large colonies) with respect to the floating cells. In the attempt to identify molecules that may play a role in supporting the observed ex vivo HSPC growth, we performed secretome analyses. We found a number of proteins involved in the HSPC homing, self-renewal, and differentiation in all tested conditions. It is important to note that a set of sixteen proteins, which are only in part reported to be expressed in any hematopoietic niche, were exclusively found in the DC system secretome. In conclusion, WJ-MSCs allowed a significant ex vivo expansion of multipotent as well as committed HSPCs. This may be relevant for future clinical applications

Comparing different methods to retrieve cloud top height from Meteosat satellite data

Cloud parameters such as the Cloud Top Height (CTH), Cloud Top Temperature (CTT), emissivity, particle size and optical depth have always been matter of interest for the atmospheric community. Particularly the CTH provides information leading to better understand the cloud radiative effects. Although there are many meteorological satellites providing the CTH, there are other sensors, not devoted to this purpose, that give some information from which this crucial parameter can be estimated. In this contribution we will describe three different methodologies to retrieve the CTH. The first technique is based on stereo-vision algorithms and requires two different views of the same scene and does not need of extra atmospheric information. In the second one, brightness temperatures in two IR spectral bands are converted to real cloud temperature by means of the proposed algorithms. From the CTT, the CTH is estimated using temperature vertical profiles (measured or modeled). The third technique retrieves the CTH from the output parameters of post event simulations performed by a Numerical Weather Prediction (NWP) model that in this work will be the mesoscale model WRF (Weather Research Forecast). This article presents a preliminary work, in which the heights retrieved by the three methodologies applied to the geostationary satellite Meteosat 10 are compared with the heights given by MODIS sensor installed on the polar satellite AQUA. This promising results show that valuable information about CTH can be retrieved from Meteosat which provide high frequency and large scale data useful for weather and climate research