Enhancement of 2,3-Butanediol Production by Klebsiella oxytoca PTCC 1402

Optimal operating parameters of 2,3-Butanediol production using Klebsiella oxytoca under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respectively. Furthermore, optimum values for temperature, inoculum size, pH, and the shaking speed were determined as 37°C, 8 (g/L), 6.1, and 150 rpm, respectively. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 44%

Genetic variation in a population of Bipolaris oryzae based on RAPD-PCR in north of Iran

Isolates of Bipolaris oryzae were analysed by RAPD techniques to determine the amount of intraspecific genetic variability. In order to do RAPD-PCR, seven primers were applied. At first, DNA of all isolates was isolated, and then DNA was amplified in thermocycler by using seven primers at a thermal program. As the result, segments of DNA by the size of 200 - 300 bp were produced. Cluster analysis using UPGMA method gave three groups. Levels of polymorphism were observed between DNA of different isolates. The pattern of RAPD bands could not show the direct correlation between polymorphism and climates or geographical areas.Keywords: Bipolaris oryzae, rice, genetic variation, RAPDAfrican Journal of Biotechnology Vol. 9(36), pp. 5800-5804, 6 September, 201