1 research outputs found
Fast, Efficient, and Stable Conjugation of Multiple DNA Strands on Colloidal Quantum Dots
A novel method for covalent conjugation
of DNA to polymer coated
quantum dots (QDs) is investigated in detail. This method is fast
and efficient: up to 12 DNA strands can be covalently conjugated per
QD in optimized reaction conditions. The QD-DNA conjugates can be
purified using size exclusion chromatography and the QDs retain high
quantum yield and excellent stability after DNA coupling. We explored
single-stranded and double-stranded DNA coupling, as well as various
lengths. We show that the DNA coupling is most efficient for short
(15 mer) single-stranded DNA. The DNA coupling has been performed
on QDs emitting at four different wavelengths, as well as on gold
nanoparticles, suggesting that this technique can be generalized to
a wide range of nanoparticles