Impact Assessment of Varied Agroclimatic Conditions on Phosphate Solubilization Potential of Fungi in Fermentation and Soil-Plant System

In this work, two phosphate solubilizing fungi viz., Aspergillus tubingensis S33 and A. niger S36 were studied under different physiochemical and nutritional parameters in the lab, and in vitro under soil-plant experiments at two very distinct agro-climatic conditions viz., Banasthali, Tonk (Rajasthan), and Dwarahat, Almora (Uttarakhand), India. Phosphate-solubilizing capability was checked with different carbon and nitrogen sources. Maltose, glucose, and fructose were optimal carbon source in A. tubingensis S33 while fructose in the case of A. awamori S33. Amongst nitrogen sources, S33 showed maximum phosphate solubilization with ammonium sulfate while, S36 with ammonium sulfate and sodium nitrate. Ammonium was more stimulating than nitrate as the chief nitrogen source. In vivo experiments revealed that solubilization was noticeable at all the temperatures, but optimal temperature was 25–35℃. The optimal initial pH for Tricalcium Phosphate (TCP) solubilization was 8.0. The ideal concentration of TCP for solubilization was 7.5 g∙l−1. The application of both strains in two different geographical sites exhibited a significant (p<0.05) rise in wheat growth, grain yield, and available Phosphorus (P). Fungal inoculation with TCP amendment exhibited a more notable effect on growth, yield, and soil fertility than control. This study support that these isolates will be able to work efficiently in varied climatic conditions and will show consistent efficiency on field application

Impact Assessment of Varied Agroclimatic Conditions on Phosphate Solubilization Potential of Fungi in Fermentation and Soil-Plant System

1267-1275In this work, two phosphate solubilizing fungi viz., Aspergillus tubingensis S33 and A. niger S36 were studied under different physiochemical and nutritional parameters in the lab, and in vitro under soil-plant experiments at two very distinct agro-climatic conditions viz., Banasthali, Tonk (Rajasthan), and Dwarahat, Almora (Uttarakhand), India. Phosphate-solubilizing capability was checked with different carbon and nitrogen sources. Maltose, glucose, and fructose were optimal carbon source in A. tubingensis S33 while fructose in the case of A. awamori S33. Amongst nitrogen sources, S33 showed maximum phosphate solubilization with ammonium sulfate while, S36 with ammonium sulfate and sodium nitrate. Ammonium was more stimulating than nitrate as the chief nitrogen source. In vivo experiments revealed that solubilization was noticeable at all the temperatures, but optimal temperature was 25–35℃. The optimal initial pH for Tricalcium Phosphate (TCP) solubilization was 8.0. The ideal concentration of TCP for solubilization was 7.5 g∙l−1. The application of both strains in two different geographical sites exhibited a significant (p<0.05) rise in wheat growth, grain yield, and available Phosphorus (P). Fungal inoculation with TCP amendment exhibited a more notable effect on growth, yield, and soil fertility than control. This study support that these isolates will be able to work efficiently in varied climatic conditions and will show consistent efficiency on field application

Germ cell depletion from mammalian ovary: possible involvement of apoptosis and autophagy

Abstract Mammalian ovary contains millions of germ cells during embryonic life but only few of them are culminated into oocytes that achieve meiotic competency just prior to ovulation. The majority of germ cells are depleted from ovary through several pathways. Follicular atresia is one of the major events that eliminate germ cells from ovary by engaging apoptotic as well as non-apoptotic pathways of programmed cell death. Apoptosis is characterized by several morphological changes that include cell shrinkage, nuclear condensation, membrane blebbing and cytoplasmic fragmentation by both mitochondria- as well as death receptor-mediated pathways in encircling granulosa cells and oocyte. Although necroapoptosis have been implicated in germ cell depletion, autophagy seems to play an active role in the life and death decisions of ovarian follicles. Autophagy is morphologically characterized by intracellular reorganization of membranes and increased number of autophagic vesicles that engulf bulk cytoplasm as well as organelles. Autophagy begins with the encapsulation of cytoplasmic constituents in a membrane sac known as autophagosomes. The autophagic vesicles are then destroyed by the lysosomal enzymes such as hydrolases that results in follicular atresia. It seems that apoptosis as well as autophagy could play active roles in germ cells depletion from ovary. Hence, it is important to prevent these two pathways in order to retain the germ cells in ovary of several mammalian species that are either threatened or at the verge of extinction. The involvement of apoptosis and autophagy in germ cell depletion from mammalian ovary is reviewed and possible pathways have been proposed

Necroptosis in stressed ovary

Abstract Stress is deeply rooted in the modern society due to limited resources and large competition to achieve the desired goal. Women are more frequently exposed to several stressors during their reproductive age that trigger generation of reactive oxygen species (ROS). Accumulation of ROS in the body causes oxidative stress (OS) and adversely affects ovarian functions. The increased OS triggers various cell death pathways in the ovary. Beside apoptosis and autophagy, OS trigger necroptosis in granulosa cell as well as in follicular oocyte. The OS could activate receptor interacting protein kinase-1(RIPK1), receptor interacting protein kinase-3 (RIPK3) and mixed lineage kinase domain-like protein (MLKL) to trigger necroptosis in mammalian ovary. The granulosa cell necroptosis may deprive follicular oocyte from nutrients, growth factors and survival factors. Under these conditions, oocyte becomes more susceptible towards OS-mediated necroptosis in the follicular oocytes. Induction of necroptosis in encircling granulosa cell and oocyte may lead to follicular atresia. Indeed, follicular atresia is one of the major events responsible for the elimination of majority of germ cells from cohort of ovary. Thus, the inhibition of necroptosis could prevent precautious germ cell depletion from ovary that may cause reproductive senescence and early menopause in several mammalian species including human