Pentobarbitone-induced sleeping time and sub-acute toxicity studies of Trichilia monadelpha aqueous extract

Background: Trichilia monadelpha is used either alone or in combination with other plants to treat many diseases in West Africa. Earlier, the anti-inflammatory, analgesic and anti-parasitic effects of the plant have been investigated to confirm its folkloric use. The current study is aimed at investigating the sub-acute toxicity profile as well as hypno-sedative effect of the Trichilia monadelpha aqueous extract (TAE).Methods: For the pentobarbitone-induced sleeping test, rats (150-200 g, n=5) were pre-treated with TAE (100, 300 and 1000 mg/kg, p.o.) or distilled water (control group) 30 minutes before they were challenged with Pentobarbitone Sodium (50 mg/kg body weight, i.p). Sleeping time of each animal was recorded and analysed. In the sub-acute toxicity test, rats were treated daily either TAE (30, 100 and 1000 mg/kg) or water (control group) for two weeks after which the animals were sacrificed. Blood samples were collected for haematological and biochemical analyses. Specific organs were then removed and weighed immediately.Results: The pentobarbitone-induced sleeping test resulted in a significant and dose-dependent increase in the duration of sleep of the rats. There were however no significant changes in the relative weight of vital organs of the control and TAE treated groups. Similarly, there were no significant differences in haematological and biochemical parameters between control and TAE treated groups.Conclusions: TAE significantly and dose-dependently increased the duration of pentobarbitone-induced sleeping time in rats. TAE showed no significant changes in the relative weight of the vital organs, haematological and biochemical parameters

Aqueous extract of Lannea microcarpa attenuates dextran sulphate-induced paw oedema and xylene-induced ear oedema in rodents

Background: Lannea microcarpa Engl. and K. Krause (Family Anacardiaceae), a tropical tree is used traditionally used in Africa for both medicinal and non-medicinal purposes. Folkoric uses of the plant include wound healing, conjunctivitis, stomatitis and gingivitis among others. Inflammatory reactions are involved in several diseases which this plant is traditionally used to manage. This study was therefore aimed at investigating the antiinflammatory effects of the aqueous extract of Lannea microcapa.Methods: Oedema was induced in the right hind paws of Sprague Dawley rats (200-250g, 12 weeks old, n=5) using dextran sulphate solution whiles ear oedema was induced in ICR mice (25-30g, 6 weeks old, n=5) using xylene solutions. Aqueous extracts of ALM (30, 100 and 300mg kg-1) were administered in a set of rats and mice for both prophylactic and therapeutic studies. In the dextran sulphate-induced paw oedema, rats (200-250g) were treated orally with ALM (30, 100 and 300 mg kg-1) for both prophylactic and therapeutic studies. The paw thickness of the rats was measured before and after dextran sulphate injection at an hourly interval for 5 h. For xylene-induced ear oedema, ICR mice (25-30g) were given the same doses of the ALM and the ear weight of mice were measured after 2 h.Results: In the dextran sulphate-induced paw oedema, the ALM reduced the mean maximal paw oedema significantly (P ≤0.05) to 36.392±9.207% and 26.050±3.396% at 100 and 300 mg kg-1 (prophylaxis) and 32.192±5.670%, 31.398±6.921% and 31.593±5.841% at 30, 100 and 300 mg kg-1 (therapeutic) in dose dependent manner when compared to the control respectively. Similarly, the ALM dose dependently showed a significant (P ≤0.05) reduction of percentage mean oedema in xylene-induced ear oedema by 43.56%, 59.63% and 68.07% at 30, 100 and 300 mg kg-1 when compared to the control respectively.Conclusions: Aqueous extract of Lannea microcapa (30 -300 mg kg-1) caused significant reduction of oedema in both dextran sulphate-induced paw oedema and xylene-induced ear oedema

Pentobarbitone-induced sleeping time and sub-acute toxicity studies of Trichilia monadelpha aqueous extract

Background: Trichilia monadelpha is used either alone or in combination with other plants to treat many diseases in West Africa. Earlier, the anti-inflammatory, analgesic and anti-parasitic effects of the plant have been investigated to confirm its folkloric use. The current study is aimed at investigating the sub-acute toxicity profile as well as hypno-sedative effect of the Trichilia monadelpha aqueous extract (TAE).Methods: For the pentobarbitone-induced sleeping test, rats (150-200 g, n=5) were pre-treated with TAE (100, 300 and 1000 mg/kg, p.o.) or distilled water (control group) 30 minutes before they were challenged with Pentobarbitone Sodium (50 mg/kg body weight, i.p). Sleeping time of each animal was recorded and analysed. In the sub-acute toxicity test, rats were treated daily either TAE (30, 100 and 1000 mg/kg) or water (control group) for two weeks after which the animals were sacrificed. Blood samples were collected for haematological and biochemical analyses. Specific organs were then removed and weighed immediately.Results: The pentobarbitone-induced sleeping test resulted in a significant and dose-dependent increase in the duration of sleep of the rats. There were however no significant changes in the relative weight of vital organs of the control and TAE treated groups. Similarly, there were no significant differences in haematological and biochemical parameters between control and TAE treated groups.Conclusions: TAE significantly and dose-dependently increased the duration of pentobarbitone-induced sleeping time in rats. TAE showed no significant changes in the relative weight of the vital organs, haematological and biochemical parameters

Modulating Effects of the Hydroethanolic Leaf Extract of Persicaria lanigera R. Br. Soják (Polygonaceae) against Acute Inflammation

Plant species have been used traditionally to treat numerous inflammatory disorders because of their known medicinal properties. This study aimed to assess the anti-inflammatory effect of aqueous ethanolic leaf extract of Persicaria lanigera using acute inflammatory models. The safety profile of the Persicaria lanigera extract was assessed using an acute toxicity model. The anti-inflammatory effect of the Persicaria lanigera leaf extract (100–600 mg·kg−1, p.o.) was studied in carrageenan-induced paw oedema, zymosan-induced knee joint arthritis, and histamine-induced paw oedema in Sprague–Dawley rats (n = 5). It was observed that the Persicaria lanigera leaf extract administered prophylactically significantly inhibited paw oedema from 99.01 ± 12.59 to 59.10 ± 4.94%, 56.08 ± 3.65%, and 48.62 ± 3.27% at 100 mg·kg−1, 300 mg·kg−1, and 600 mg·kg−1, while the standard drug, aspirin, showed 41.84 ± 9.25% in carrageenan-induced paw oedema, respectively. Furthermore, the extract decreased knee joint inflammation significantly from 62.43 ± 5.73% to 32.07 ± 2.98% and 24.33 ± 8.58% at 300 mg·kg−1 and 600 mg·kg−1 in zymosan-induced knee joint inflammation, respectively. In the histamine-induced paw oedema model, the extract significantly inhibited oedema to 61.53 ± 9.17%, 54.21 ± 9.38%, and 54.22 ± 9.37% at the same doses. Aqueous ethanolic leaf extract of Persicaria lanigera is safe and attenuates inflammation in acute inflammation models