The ALE-method with triangular elements: direct convection of integration point values

The arbitrary Lagrangian-Eulerian (ALE) finite element method is applied to the simulation of forming processes where material is highly deformed. Here, the split formulation is used: a Lagrangian step is done with an implicit finite element formulation, followed by an explicit (purely convective) Eulerian step. The purpose of this study is to investigate the Eulerian step for quadratic triangular elements. To solve the convection equation for integration point values, a new method inspired by Van Leer is constructed. The new method is based on direct convection of integration point values without intervention of nodal point values.\ud The Molenkamp test and a so-called block test were executed to check the performance and stability of the convection scheme. From these tests it is concluded that the new convection scheme shows accurate results. The scheme is extended to an ALE-algorithm. An extrusion process was simulated to test the applicability of the scheme to engineering problems. It is concluded that direct convection of integration point values with the presented algorithm leads to accurate results and that it can be applied to ALE-simulation

K Channel Subconductance Levels Result from Heteromeric Pore Conformations

Voltage-gated K channels assemble from four identical subunits symmetrically arranged around a central permeation pathway. Each subunit harbors a voltage-sensing domain. The sigmoidal nature of the activation kinetics suggests that multiple sensors need to undergo a conformational change before the channel can open. Following activation, individual K channels alternate stochastically between two main permeation states, open and closed. This binary character of single channel behavior suggests the presence of a structure in the permeation pathway that can exist in only two conformations. However, single channel analysis of drk1 (Kv2.1) K channels demonstrated the existence of four additional, intermediate conductance levels. These short-lived subconductance levels are visited when the channel gate moves between the closed and fully open state. We have proposed that these sublevels arise from transient heteromeric pore conformations, in which some, but not all, subunits are in the “open” state. A minimal model based on this hypothesis relates specific subconductance states with the number of activated subunits (Chapman et al., 1997). To stringently test this hypothesis, we constructed a tandem dimer that links two K channel subunits with different activation thresholds. Activation of this dimer by strong depolarizations resulted in the characteristic binary open–close behavior. However, depolarizations to membrane potentials in between the activation thresholds of the two parents elicited highly unusual single channel gating, displaying frequent visits to two subconductance levels. The voltage dependence and kinetics of the small and large sublevels associate them with the activation of one and two subunits, respectively. The data therefore support the hypothesis that subconductance levels result from heteromeric pore conformations. In this model, both sensor movement and channel opening have a subunit basis and these processes are allosterically coupled

Discrete Multitone Modulation for Maximizing Transmission Rate in Step-Index Plastic Optical Fibres

The use of standard 1-mm core-diameter step-index plastic optical fiber (SI-POF) has so far been mainly limited to distances of up to 100 m and bit-rates in the order of 100 Mbit/s. By use of digital signal processing, transmission performance of such optical links can be improved. Among the different technical solutions proposed, a promising one is based on the use of discrete multitone (DMT) modulation, directly applied to intensity-modulated, direct detection (IM/DD) SI-POF links. This paper presents an overview of DMT over SI-POF and demonstrates how DMT can be used to improve transmission rate in such IM/DD systems. The achievable capacity of an SI-POF channel is first analyzed theoretically and then validated by experimental results. Additionally, first experimental demonstrations of a real-time DMT over SI-POF system are presented and discusse

The arbitrary Lagrangian-Eulerian method on grids of triangular elements: a new convection scheme

This study investigates the Eulerian step of a split ALE nite element method for quadratic triangular elements. To solve the convection equation for integration point values, a new method is constructed, directly based on integration point values without using intermediate nodal values. The Molenkamp test and a so-called block test were executed to check the performance and stability of the convection scheme. From these tests it is concluded that the new convection scheme shows accurate results

A geometrical-based contact algorithm using a barrier method

Most methods employed in the numerical solution of contact problems in finite element simulations rely on equality-based optimization methods. Typically, a gap function which is non-differentiable at the point of contact is used in these kind of approaches. The gap function can be seen as the Macaulay bracket of some distance function, where the latter is differentiable at the point of contact. In this article, we propose to use the distance function directly instead of using the gap function. This will give rise to a formulation involving inequality constraints. This approach eliminates the artificially introduced non-differentiability. To this end we propose a barrier algorithm as the method of choice to solve the problem. The method originates in optimization literature, where convergence proofs for the method are availabl

Genetic Correction of SOD1 Mutant iPSCs Reveals ERK and JNK Activated AP1 as a Driver of Neurodegeneration in Amyotrophic Lateral Sclerosis

Summary: Although mutations in several genes with diverse functions have been known to cause amyotrophic lateral sclerosis (ALS), it is unknown to what extent causal mutations impinge on common pathways that drive motor neuron (MN)-specific neurodegeneration. In this study, we combined induced pluripotent stem cells-based disease modeling with genome engineering and deep RNA sequencing to identify pathways dysregulated by mutant SOD1 in human MNs. Gene expression profiling and pathway analysis followed by pharmacological screening identified activated ERK and JNK signaling as key drivers of neurodegeneration in mutant SOD1 MNs. The AP1 complex member JUN, an ERK/JNK downstream target, was observed to be highly expressed in MNs compared with non-MNs, providing a mechanistic insight into the specific degeneration of MNs. Importantly, investigations of mutant FUS MNs identified activated p38 and ERK, indicating that network perturbations induced by ALS-causing mutations converge partly on a few specific pathways that are drug responsive and provide immense therapeutic potential. : In this article, Bhinge, Stanton, and colleagues use genome editing of patient-derived iPSCs to model ALS phenotypic defects in vitro. Transcriptomic analysis of disease MNs reveals activation of MAPK, AP1, WNT, cell-cycle, and p53 signaling in ALS MNs. Pharmacological screening uncovers activated ERK and JNK signaling as therapeutic targets in ALS. Keywords: ALS, SOD1, FUS, CRISPR-Cas9, p38, ERK, JNK, WNT, TP53, JU

Author Correction: Reconfigurable beam system for non-line-of-sight free-space optical communication (Light: Science & Applications, (2019), 8, 1, (69), 10.1038/s41377-019-0177-3)

Addendum: We were recently made aware of the fact that Professor Nabeel A. Riza’s laboratory theoretically proposed earlier a Non-Line of Sight (NLOS) indoor optical wireless communications arrangement with threedimensional optical beamforming via a spatial light modulator using diffuse reflections from diffuse optical elements1. This is exactly the type of arrangement that would benefit greatly from our technique, since the coherent arrayed optical transmitter (CAO-Tx) we proposed makes the reflection focus onto the receiver, rather than diffusely scatter it in all directions. We apologize for the omission of this prior-art

Reconfigurable beam system for non-line-of-sight free-space optical communication

In this paper, we propose a reconfigurable beam-shaping system to permit energy-efficient non-line-of-sight (NLOS) free-space optical communication. Light is steered around obstacles blocking the direct communication pathway and reaches a receiver after reflecting off of a diffuse surface. A coherent array optical transmitter (CAO-Tx) is used to spatially shape the wavefront of the light incident on a diffuse surface. Wavefront shaping is used to enhance the amount of diffusely reflected light reaching the optical receiver. Synthetic NLOS experiments for a signal reflected over an angular range of 20° are presented. A record-breaking 30-Gbit/s orthogonal frequency-division multiplexing signal is transmitted over a diffused optical wireless link with a >17-dB gain