Incidence rate ratio (95% confidence interval) of different activities for treatment group compared to control group: mixed effects poisson regression analysis that adjusted for offspring weight, gender, random selection, and testing time and day.

<p>P<0.008 was considered significant.</p

Cortical neuron densities in Nissl-stained frontal cortex of offspring between control and APAP groups.

<p>Whole brains were washed and processed for Nissl staining by immersing in 30% sucrose until saturation and cryosectioned at 20 μm thickness. Neurons were counted (field of view) based on randomly chosen 5 fields in frontal cortex per animal. The neurons were identified by a large cell body or perikaryon containing a large, pale nucleus with a prominent dark nucleolus. Experiments were performed in 3–5 repeats for all groups. There was no significant difference in cortical neuron density between control and treated groups (p < 0.05; Unpaired Student’s T-Test; n = 5 control, n = 3 treated). Data are presented as the mean±s.e.m. Statistical significance was determined using Student’s unpaired t-test.</p

Serum ALT concentration in dams from control and APAP groups at ED 7 and 18.

<p>Serum ALT concentration in dams from control and APAP groups at ED 7 and 18.</p

Mean values of different activities according to group assignment.

<p>Mean values of different activities according to group assignment.</p