SNPs that modify miRNA binding sites according to PolymiRTS Database.

<p>PolymiRTS Database uses the criteria of TargetScan for miRNA binding sites prediction.</p>(a)<p>Support column indicates occurrence of the miRNA site in other vertebrate genomes in addition to the query genome.</p>(b)<p>Function Class specifies if the derived allele either disrupts a non conserved miRNA site (N) or creates a new miRNA site (C).</p

Allele and genotype frequencies of <i>BDNF</i> polymorphism rs6265 (Val66Met) in control subjects and schizophrenic patients.

<p>Allele and genotype frequencies of <i>BDNF</i> polymorphism rs6265 (Val66Met) in control subjects and schizophrenic patients.</p

Luciferase assays for validation of miR-26a and -26b binding to <i>BDNF</i> 3′UTR.

<p>HeLa cells were independently transfected with control plasmid (pRL-TK) or each of the two reporter plasmid (pluc-BDNF C–G, anc, and pluc-BDNF A–T, der) with either miR-26a or miR-26b. Data are presented as the normalized activity of different reporter genes. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028656#s1" target="_blank">Introduction</a> of exogenous miR-26a and miR-26b represses reporter activity of pluc-BDNF C–G but has no effect on pluc-BDNF A–T. Data represent the mean of five independent experiments +SD (<i>p</i><0.05).</p

Analysis output with miRecords, that integrates predicted miRNA targets produced by 11 miRNA target prediction programs.

<p>Here are reported programs that predict <i>BDNF</i> 3′UTR miRna binding sites for miRNAs identified with PolymiRTS. Other programs are: DIANA-microT, MicroInspector, MirTarget2, miTarget, NBmiRTar, PicTar, TargetScan and RNA22.</p

Schematic representation of base pairing between miR-26a sequence and <i>BDNF</i> 3′UTR ancestral (C–G) and derivative (A–T) alleles of rs11030100 and rs11030099 polymorphic sites.

<p>MiR-26b has the same seed binding sequence.</p

Additional file 1: of Crizotinib-induced antitumour activity in human alveolar rhabdomyosarcoma cells is not solely dependent on ALK and MET inhibition

ALK and MET knock-down by RNA interference. A: RH4 and RH30 cells were transfected with either scramble control siRNA (NC siRNA) or ALK siRNA. Cells were harvested 48 h after transfection and ALK, AKT (phosphorylated and total protein) and ERK (phosphorylated and total protein) levels were analysed by Western blotting. B: RH4 and RH30 cells were transfected with either scramble negative control siRNA (NC siRNA) or MET siRNA. Cells were harvested 48 h after transfection and MET, AKT (phosphorylated and total protein) and ERK (phosphorylated and total protein) levels were analysed by Western blotting. Tubulin was used as loading control in all experiments. (TIFF 258 kb