Predictive values for the diagnosis of biopsy-proven HSIL.

<p>Predictive values for the diagnosis of biopsy-proven HSIL.</p

Logistic regression analysis.

<p>Outcome: Biopsy-proven HSIL.</p

ROC area for diagnosis of biopsy proven-HSIL of the combination of the standard cytology with P16/Ki67 immunostaining vs. standard cytology alone.

<p>ROC area for diagnosis of biopsy proven-HSIL of the combination of the standard cytology with P16/Ki67 immunostaining vs. standard cytology alone.</p

Frequencies of P16/Ki67 positive immunostaining according to histologic results.

<p>Frequencies of P16/Ki67 positive immunostaining according to histologic results.</p

Predictive values for the diagnosis of biopsy-proven HSIL.

<p>Predictive values for the diagnosis of biopsy-proven HSIL.</p

General characteristics of the study population.

<p>General characteristics of the study population.</p

Novel splice junctions identified in this study.

<p>Consensus sequences at both sides of splice junctions are shown. Splice sites involved in splice junctions are indicated, with HXB2 positions in parentheses. Nearby splice sites used in the corresponding samples are also indicated.</p

Clinical data and subtypes of samples used in this study.

<p>Clinical data and subtypes of samples used in this study.</p

Phylogenetic tree of DS HIV-1 RNA sequences.

<p>The analysis was done with the fragment comprising exons 5 and 7 (HXB2 positions 5977–6045; 8379–8533), common to all DS transcripts, using 20 randomly chosen sequences per sample. Clades comprising sequences from each sample are compressed in triangles. SH-like node support values for sample clades and for subtype clades are shown.</p

Sequences surrounding newly identified 3’ss used for splicing at the 3’-terminal exon of DS RNAs.

<p>Sequences correspond to consensuses of exon 7 of DS RNAs of the corresponding samples. The HXB2 reference sequence is shown on top and names and positions in the HXB2 proviral genome of 3’ss are indicated above the alignment. AG dinucleotides immediately upstream of these sites are in red and pyrimidine-rich tracts upstream of these AGs are underlined. Nucleotides corresponding to the new 3’ss are boxed. The yUnAy human consensus branch point sequence present upstream of the newly identified sites is shown in blue. AG, pyrimidine-rich tract, and yUnAy motif are also signaled in SPX21, although no usage of new 3’ss was detected in this sample.</p