Clinical events rate at a median FU of 5 years (P refers to pooled OR).

<p>Clinical events rate at a median FU of 5 years (P refers to pooled OR).</p

Major adverse cardiovascular events at a median FU of 5 years, mean 5.6 years (from top to bottom, MACE, Death, MI, Stroke, TVR).

<p>Major adverse cardiovascular events at a median FU of 5 years, mean 5.6 years (from top to bottom, MACE, Death, MI, Stroke, TVR).</p

Major adverse cardiovascular events at 1 year (from top to bottom, MACE, Death, MI, Stroke, TVR).

<p>Major adverse cardiovascular events at 1 year (from top to bottom, MACE, Death, MI, Stroke, TVR).</p

Features of included studies.

<p>CABG: Coronary artery bypass graft; CVA; cerebrovascular accident; DES: drug eluting stent; LAD: left anterior descending; MI: myocardial infarction; PCI: percutaneous coronary intervention; RCT: randomised controlled trial.</p

Design features and appraisal of the internal validity of included studies.

<p>Risk of bias is expressed as A (low risk), B (moderate risk), C (high risk), and D (incomplete reporting leading to inability to ascertain the underlying risk of bias).</p

Patients and procedural features of included studies.

<p>Patients and procedural features of included studies.</p

<i>In vivo</i> effects of PCB-126 and genistein on vitellogenin expression in zebrafish

<p>In this study, the vitellogenin (Vtg) modulation by genistein and polychlorinated biphenyl-126 (PCB-126) exposure in zebrafishes has been investigated. Both PCB-126 and genistein have been identified as aquatic pollutants and can further increase estrogenicity of waterways. Vtg is egg yolk precursor protein release by the hepatocytes during vitellogenesis. This process occurs normally in the hepatocytes in response to the activation with the estrogens such as 17-β-estradiol. Our immunohistochemical findings showed a Vtg expression that increases at 12 h and at 72 h in the liver of treated fishes with both PCB-126 and genistein, individually and in combination. Furthermore, for the first time, also hepatic stellate cells (HSC) in the liver parenchyma were strongly positive for vitellogenin.</p

Confocal microscopy on human atherosclerotic carotid sections.

<p>The macroscopic aspect of a carotid plaque is reconstructed in A by stereomicroscope and displays a lipidic core and of luminal thrombus. Features are confirmed in B by histology (haematoxylin and eosin). Confocal microscopy in C shows a reconstruction of part of the area close to the lumen (L) in a section stained with Fab7816-FLAG (green), mouse-anti-human Collagen type I (white) and mouse anti-human CD45 (red), revealed by opportune secondary antibodies. In D is an enlargement of the area indicated by § symbol in C with several Fab7816-FLAG+/CD45+ cells in the neointima. indicate a regions magnified. Scale bars indicate the magnification.</p

Cross reactivity of commercial available monoclonal antibodies with TAGLN and OMPs.

<p>A) WB of OmpK36 with five commercial monoclonal mouse anti-human TAGLN antibodies (used at 1 or 5 <b>µ</b>g/ml). Three commercial mouse monoclonal antibodies were used as negative controls. Anti-6xHIS antibody (Roche) was used as positive control B) ELISA with all representative and five commercial monoclonal mouse anti-human TAGLN antibodies on purified human TAGLN or C) bacterial OMPs. Reactivity against bovine serum albumin (BSA) used as blocking antigen, is also shown.</p

Immunofluorescence on human coronary plaqes and Immunohistochemistry on human carotid sections with Fab7816-FLAG.

<p>a) Representative section stained by Movat’s pentchrome (left panel) showed the morphology of a portion of the coronary plaque tissue (plaque ID-A) displaying slightly damaged media rich in smooth muscle cells. Confocal microscopy (right panels) showed the presence of CD45⁺ cells (red) labelled by Fab7816-FLAG revealed by MAb M2 anti-FLAG-FITC (green) in the coronary plaque tissue region indicated by symbol (#). DAPI stained the nuclei (blue). b) Immunoperoxidase on carotid plaque samples demonstrated the presence of several cells reacting with Fab7816-FLAG in an area close to the lumen (left panels) as revealed by MAb M2 anti-FLAG-HRP developed with DAB (brown). The signal is absent in a serial section where the Fab7816-FLAG is omitted (ctrl-, right panels). Magnified images in the bottom panels evidence the spindle shape of Fab7816-FLAG+ cells and their localization in between a clusters of altered cells, possibly foam cell. Haematoxylin (blue) stains nuclei. Scale bars indicate the magnification.</p