Changes in gastric pH as a function of the wet weight in fed larvae, juveniles and adults of <i>Diplodus sargus</i>.

<p>Lines indicate the regression of pH in relation to wet weight (W) in fed animals.</p

Pepsin activity (mean ± SD) during the 24 h cycle in juveniles of <i>Diplodus sargus</i>.

<p>Red line indicates the analyses done at pH 2. Black line indicates the analyses done at the actual luminal pH. Same letter indicates no significant difference (<i>P</i>>0.05). Green area: feeding period. Grey area: dark period.</p

Gene expression of pepsinogen and H⁺/K⁺-ATPase mRNA during the 24 h cycle in juveniles of <i>Diplodus sargus</i>.

<p>Same letter indicates no significant difference (<i>P</i>>0.05); a and b: proton pump; a′ and b′: pepsinogen. Green area: feeding period. Grey area: dark period.</p

Oligonucleotides designed for real time RT-PCR.

<p>Oligonucleotides designed for real time RT-PCR.</p

Evolutionary relationships of 40 taxa for pepsinogen precursors.

<p>The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test is shown next to the branches. GenBank or NCBI Reference Sequence accession number appears to the right of each taxon.</p

Evolutionary relationships of 28 taxa for proton/potassium and sodium/potassium pumps.

<p>The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test is shown next to the branches. GenBank or NCBI Reference Sequence accession number appears to the right of each taxon.</p

Relative stomach fullness during the 24 h cycle in juveniles of <i>Diplodus sargu</i>s.

<p>Green area: feeding period. Grey area: dark period.</p