Comparison of the performance of Skin Prick, ImmunoCAP, and ISAC tests in the diagnosis of patients with allergy

Background: Allergy is diagnosed from typical symptoms, and tests are performed to incriminate the suspected precipitant. Skin prick tests (SPTs) are commonly performed, inexpensive, and give immediate results. Laboratory tests (ImmunoCAP) for serum allergen-specific IgE antibodies are usually performed more selectively. The immuno-solid phase allergen chip (ISAC) enables testing for specific IgE against multiple allergen components in a multiplex assay. Methods: We retrospectively analysed clinic letters, case notes, and laboratory results of 118 patients attending the National Adult Allergy Service at the University Hospital of Wales who presented diagnostic difficulty, to evaluate which testing strategy (SPT, ImmunoCAP, or ISAC) was the most appropriate to use to confirm the diagnosis in these complex patients, evaluated in a “real-life” clinical service setting. Results: In patients with nut allergy, the detection rates of SPTs (56%) and ISAC (65%) were lower than those of ImmunoCAP (71%). ISAC had a higher detection rate (88%) than ImmunoCAP (69%) or SPT (33%) in the diagnosis of oral allergy syndrome. ImmunoCAP test results identified all 9 patients with anaphylaxis due to wheat allergy (100%), whereas ISAC was positive in only 6 of these 9 (67%). Conclusions: In this difficult diagnostic group, the ImmunoCAP test should be the preferred single test for possible allergy to nuts, wheat, other specific foods, and anaphylaxis of any cause. In these conditions, SPT and ISAC tests give comparable results. The most useful single test for oral allergy syndrome is ISAC, and SPT should be the preferred test for latex allergy

Stimuli Responsive Hybrid Magnets: Tuning the Photoinduced Spin-Crossover in Fe(III) Complexes Inserted into Layered Magnets

The insertion of a [Fe­(sal₂-trien)]⁺ complex cation into a 2D oxalate network in the presence of different solvents results in a family of hybrid magnets with coexistence of magnetic ordering and photoinduced spin-crossover (LIESST effect) in compounds [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHCl₃ (<b>1·CHCl</b>_<b>3</b>), [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHBr₃ (<b>1·CHBr</b>_<b>3</b>), and [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CH₂Br₂ (<b>1·CH</b>_<b>2</b><b>Br</b>_<b>2</b>). The three compounds crystallize in a 2D honeycomb anionic layer formed by Mn^II and Cr^III ions linked through oxalate ligands and a layer of [Fe­(sal₂-trien)]⁺ complexes and solvent molecules (CHCl₃, CHBr₃, or CH₂Br₂) intercalated between the 2D oxalate network. The magnetic properties and Mössbauer spectroscopy indicate that they undergo long-range ferromagnetic ordering at 5.6 K and a spin crossover of the intercalated [Fe­(sal₂-trien)]⁺ complexes at different temperatures <i>T</i>_1/2. The three compounds present a LIESST effect with a relaxation temperature <i>T</i>_LIESST inversely proportional to <i>T</i>_1/2. The isostructural paramagnetic compound, [Fe^III(sal₂-trien)]­[Zn^IICr^III(ox)₃]·CH₂Cl₂ (<b>2·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) was also prepared. This compound presents a partial spin crossover of the inserted Fe^III complex as well as a LIESST effect. Finally, spectroscopic characterization of the Fe^III doped compound [Ga_0.99Fe_0.01(sal₂trien)]­[Mn^IICr^III(ox)₃]·CH₂Cl₂ (<b>3·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) shows a gradual and complete thermal spin crossover and a LIESST effect on the isolated Fe^III complexes. This result confirms that cooperativity is not a necessary condition to observe the LIESST effect in an Fe^III compound

Stimuli Responsive Hybrid Magnets: Tuning the Photoinduced Spin-Crossover in Fe(III) Complexes Inserted into Layered Magnets

The insertion of a [Fe­(sal₂-trien)]⁺ complex cation into a 2D oxalate network in the presence of different solvents results in a family of hybrid magnets with coexistence of magnetic ordering and photoinduced spin-crossover (LIESST effect) in compounds [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHCl₃ (<b>1·CHCl</b>_<b>3</b>), [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHBr₃ (<b>1·CHBr</b>_<b>3</b>), and [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CH₂Br₂ (<b>1·CH</b>_<b>2</b><b>Br</b>_<b>2</b>). The three compounds crystallize in a 2D honeycomb anionic layer formed by Mn^II and Cr^III ions linked through oxalate ligands and a layer of [Fe­(sal₂-trien)]⁺ complexes and solvent molecules (CHCl₃, CHBr₃, or CH₂Br₂) intercalated between the 2D oxalate network. The magnetic properties and Mössbauer spectroscopy indicate that they undergo long-range ferromagnetic ordering at 5.6 K and a spin crossover of the intercalated [Fe­(sal₂-trien)]⁺ complexes at different temperatures <i>T</i>_1/2. The three compounds present a LIESST effect with a relaxation temperature <i>T</i>_LIESST inversely proportional to <i>T</i>_1/2. The isostructural paramagnetic compound, [Fe^III(sal₂-trien)]­[Zn^IICr^III(ox)₃]·CH₂Cl₂ (<b>2·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) was also prepared. This compound presents a partial spin crossover of the inserted Fe^III complex as well as a LIESST effect. Finally, spectroscopic characterization of the Fe^III doped compound [Ga_0.99Fe_0.01(sal₂trien)]­[Mn^IICr^III(ox)₃]·CH₂Cl₂ (<b>3·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) shows a gradual and complete thermal spin crossover and a LIESST effect on the isolated Fe^III complexes. This result confirms that cooperativity is not a necessary condition to observe the LIESST effect in an Fe^III compound

Stimuli Responsive Hybrid Magnets: Tuning the Photoinduced Spin-Crossover in Fe(III) Complexes Inserted into Layered Magnets

The insertion of a [Fe­(sal₂-trien)]⁺ complex cation into a 2D oxalate network in the presence of different solvents results in a family of hybrid magnets with coexistence of magnetic ordering and photoinduced spin-crossover (LIESST effect) in compounds [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHCl₃ (<b>1·CHCl</b>_<b>3</b>), [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHBr₃ (<b>1·CHBr</b>_<b>3</b>), and [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CH₂Br₂ (<b>1·CH</b>_<b>2</b><b>Br</b>_<b>2</b>). The three compounds crystallize in a 2D honeycomb anionic layer formed by Mn^II and Cr^III ions linked through oxalate ligands and a layer of [Fe­(sal₂-trien)]⁺ complexes and solvent molecules (CHCl₃, CHBr₃, or CH₂Br₂) intercalated between the 2D oxalate network. The magnetic properties and Mössbauer spectroscopy indicate that they undergo long-range ferromagnetic ordering at 5.6 K and a spin crossover of the intercalated [Fe­(sal₂-trien)]⁺ complexes at different temperatures <i>T</i>_1/2. The three compounds present a LIESST effect with a relaxation temperature <i>T</i>_LIESST inversely proportional to <i>T</i>_1/2. The isostructural paramagnetic compound, [Fe^III(sal₂-trien)]­[Zn^IICr^III(ox)₃]·CH₂Cl₂ (<b>2·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) was also prepared. This compound presents a partial spin crossover of the inserted Fe^III complex as well as a LIESST effect. Finally, spectroscopic characterization of the Fe^III doped compound [Ga_0.99Fe_0.01(sal₂trien)]­[Mn^IICr^III(ox)₃]·CH₂Cl₂ (<b>3·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) shows a gradual and complete thermal spin crossover and a LIESST effect on the isolated Fe^III complexes. This result confirms that cooperativity is not a necessary condition to observe the LIESST effect in an Fe^III compound

Stimuli Responsive Hybrid Magnets: Tuning the Photoinduced Spin-Crossover in Fe(III) Complexes Inserted into Layered Magnets

The insertion of a [Fe­(sal₂-trien)]⁺ complex cation into a 2D oxalate network in the presence of different solvents results in a family of hybrid magnets with coexistence of magnetic ordering and photoinduced spin-crossover (LIESST effect) in compounds [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHCl₃ (<b>1·CHCl</b>_<b>3</b>), [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CHBr₃ (<b>1·CHBr</b>_<b>3</b>), and [Fe^III(sal₂-trien)]­[Mn^IICr^III(ox)₃]·CH₂Br₂ (<b>1·CH</b>_<b>2</b><b>Br</b>_<b>2</b>). The three compounds crystallize in a 2D honeycomb anionic layer formed by Mn^II and Cr^III ions linked through oxalate ligands and a layer of [Fe­(sal₂-trien)]⁺ complexes and solvent molecules (CHCl₃, CHBr₃, or CH₂Br₂) intercalated between the 2D oxalate network. The magnetic properties and Mössbauer spectroscopy indicate that they undergo long-range ferromagnetic ordering at 5.6 K and a spin crossover of the intercalated [Fe­(sal₂-trien)]⁺ complexes at different temperatures <i>T</i>_1/2. The three compounds present a LIESST effect with a relaxation temperature <i>T</i>_LIESST inversely proportional to <i>T</i>_1/2. The isostructural paramagnetic compound, [Fe^III(sal₂-trien)]­[Zn^IICr^III(ox)₃]·CH₂Cl₂ (<b>2·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) was also prepared. This compound presents a partial spin crossover of the inserted Fe^III complex as well as a LIESST effect. Finally, spectroscopic characterization of the Fe^III doped compound [Ga_0.99Fe_0.01(sal₂trien)]­[Mn^IICr^III(ox)₃]·CH₂Cl₂ (<b>3·CH</b>_<b>2</b><b>Cl</b>_<b>2</b>) shows a gradual and complete thermal spin crossover and a LIESST effect on the isolated Fe^III complexes. This result confirms that cooperativity is not a necessary condition to observe the LIESST effect in an Fe^III compound