Virulence-associated gene profiles of <i>P. multocida</i> isolates from chimpanzees.

<p>+: positive; −: negative.</p

Radial Maximum Likelihood trees constructed with concatenated housekeeping gene allele sequences used for MLST analysis.

<p>Shown left is the complete MLST tree constructed with 73 sequence types (STs) and the position of the type strains for the <i>P. multocida</i> subspecies (<i>P. multocida</i> ssp. <i>septica</i>: CIPA125; <i>P. multocida</i> ssp. <i>multocida</i>: NCTC10322; <i>P. multocida</i> ssp. <i>gallicida</i>: NCTC10204). The position of the chimpanzee isolates (boxed grey) is displayed in the subtree (A). STs are labelled with coloured dots indicating the isolation sources and ST numbers. Bold branches indicate for bootstrap values >70%.</p

Antibiogram of <i>P. multocida</i> isolates (according to the standards given by CLSI [56]).

<p>S: susceptible; R: resistant; I: intermediate.</p

Origins and characteristics of <i>Pasteurella multocida</i> isolated in this study.

a<p>Identified by PCR <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024236#pone.0024236-Chi1" target="_blank">[24]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024236#pone.0024236-Kndgen1" target="_blank">[25]</a>.</p>b<p>Identified by cultivation and subsequent phenotypical characterization.</p><p>HMPV  =  Human Metapneumovirus</p

Dendrogram showing macrorestriction patterns of <i>P. multocida</i> isolates after digestion with <i>SmaI</i>.

<p>Cluster analysis of Dice similarity indices (UPGMA) was exerted to generate a dendrogram depicting the relationships among PFGE profiles using the BioNumerics software (optimisation 0.5%, position tolerance 1.0%). <i>P. multocida</i> reference strain ATCC 43137 served as methodological control.</p

Phylogenetic analysis of the <i>sodA</i> gene.

<p>The tree was built using the maximum-likelihood method from an analysis of <i>sodA</i> sequences (452 bp) from the chimpanzee's isolates and sequences obtained from GenBank. Isolates belonging to the species <i>P. multocida</i> are boxed grey; the chimpanzees isolates are written in bold. Taxon labels indicate species and strain number, including strains with identical <i>sodA</i> sequences (species and subspecies assignments are according to Gautier et al. (2005) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024236#pone.0024236-Gautier1" target="_blank">[28]</a>). <i>P. langaensis</i> and <i>P. bettyae</i> were used as outgroup. Bootstrap values were calculated with 500 replicates and are given in percent.</p

Biochemical profiles of <i>P. multocida</i> isolates from chimpanzees.

<p>+: positive; −: negative; +/−: ambiguous result.</p

<i>Spa</i> type frequency and predicted clonal complexes based on <i>spa</i> typing and MLST for representative MRSA from wound infections of canine, feline and equine origin in Germany.

<p><i>Spa</i> type frequency and predicted clonal complexes based on <i>spa</i> typing and MLST for representative MRSA from wound infections of canine, feline and equine origin in Germany.</p

Overview of lineage-diversity among MRSA from dogs, cats and horses.

<p>Overview of lineage-diversity among MRSA from dogs, cats and horses.</p

<i>S. aureus</i> proportion in wound samples from companion animals.

<p><i>S. aureus</i> proportion in wound samples from companion animals.</p