Oxidation reaction of NMDA by D-Aspartate oxidase and production of methylamine (CHNH)

<p><b>Copyright information:</b></p><p>Taken from "-Methyl-D-aspartic Acid (NMDA) in the nervous system of the amphioxus "</p><p>http://www.biomedcentral.com/1471-2202/8/109</p><p>BMC Neuroscience 2007;8():109-109.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2241627.</p><p></p

-Methyl-D-aspartic Acid (NMDA) in the nervous system of the amphioxus -1

<p><b>Copyright information:</b></p><p>Taken from "-Methyl-D-aspartic Acid (NMDA) in the nervous system of the amphioxus "</p><p>http://www.biomedcentral.com/1471-2202/8/109</p><p>BMC Neuroscience 2007;8():109-109.</p><p>Published online 20 Dec 2007</p><p>PMCID:PMC2241627.</p><p></p>before purification by OPA treatment. The same sample after purification with OPA, which eliminates all the amino acids (or almost all) except NMDA. Note that it is not possible to see the NMDA in this graphic because it does not react with OPA-mercaptoethanol, that is the reagent used for the determination of free amino acids at HPLC. The same sample as B, but after treatment with D-AspO. In this case, the D-AspO oxidizes NMDA producing the CHNHwhich reacts with OPA-mercaptoethanol to give a well-defined sharp peak at the end of the chromatogram at retention time 11.8–12.0 min