Identification of a New Family of Enzymes with Potential \u3cem\u3eO\u3c/em\u3e-acetylpeptidoglycan esterase activity in both Gram-positive and Gram-negative bacteria

Background: The metabolism of the rigid bacterial cell wall heteropolymer peptidoglycan is a dynamic process requiring continuous biosynthesis and maintenance involving the coordination of both lytic and synthetic enzymes. The O-acetylation of peptidoglycan has been proposed to provide one level of control on these activities as this modification inhibits the action of the major endogenous lytic enzymes, the lytic transglycosylases. The O-acetylation of peptidoglycan also inhibits the activity of the lysozymes which serve as the first line of defense of host cells against the invasion of bacterial pathogens. Despite this central importance, there is a dearth of information regarding peptidoglycan O-acetylation and nothing has previously been reported on its de-acetylation. Results: Homology searches of the genome databases have permitted this first report on the identification of a potential family of O-Acetylpeptidoglycan esterases (Ape). These proteins encoded in the genomes of a variety of both Gram-negative and Gram-positive bacteria, including a number of important human pathogens such as species of Neisseria, Helicobacter, Campylobacter, and Bacillus anthracis, have been organized into three families based on amino acid sequence similarities with family 1 being further divided into three sub-families. The genes encoding these proteins are shown to be clustered with Peptidoglycan O-acetyltransferases (Pat) and in some cases, together with other genes involved in cell wall metabolism. Representative bacteria that encode the Ape proteins were experimentally shown to produce O-acetylated peptidoglycan. Conclusion: The hypothetical proteins encoded by the pat and ape genes have been organized into families based on sequence similarities. The Pat proteins have sequence similarity to Pseudomonas aeruginosa AlgI, an integral membrane protein known to participate in the O-acetylation of the exopolysaccaride, alginate. As none of the bacteria that harbor the pat genes produce alginate, we propose that the Pat proteins serve to O-acetylate peptidoglycan which is known to be a maturation event occurring in the periplasm. The Ape sequences have amino acid sequence similarity to the CAZy CE 3 carbohydrate esterases, a family previously known to be composed of only O-acetylxylan esterases. They are predicted to contain the α/β hydrolase fold associated with the GDSL and TesA hydrolases and they possess the signature motifs associated with the catalytic residues of the CE3 esterases. Specific signature sequence motifs were identified for the Ape proteins which led to their organization into distinct families. We propose that by expressing both Pat and Ape enzymes, bacteria would be able to obtain a high level of localized control over the degradation of peptidoglycan through the attachment and removal of O-linked acetate. This would facilitate the efficient insertion of pores and flagella, localize spore formation, and control the level of general peptidoglycan turnover

Evidence that particle acceleration in hotspots of FR II galaxies is not constrained by synchrotron cooling

We study the hotspots of powerful radiogalaxies, where electrons accelerated at the jet termination shock emit synchrotron radiation. The turnover of the synchrotron spectrum is typically observed between infrared and optical frequencies, indicating that the maximum energy of non-thermal electrons accelerated at the shock is ~TeV for a canonical magnetic field of ~100 micro Gauss. We show that this maximum energy cannot be constrained by synchrotron losses as usually assumed, unless the jet density is unreasonably large and most of the jet upstream energy goes to non-thermal particles. We test this result by considering a sample of hotspots observed at radio, infrared and optical wavelengths.Comment: 7 pages, 2 figures. To be appear in the proceedings of the conference "Cosmic ray origin - beyond the standard models" (San Vito di Cadore, Italy, September 2016

Particle acceleration and magnetic field amplification in the jets of 4C74.26

We model the multi-wavelength emission in the southern hotspot of the radio quasar 4C74.26. The synchrotron radio emission is resolved near the shock with the MERLIN radio-interferometer, and the rapid decay of this emission behind the shock is interpreted as the decay of the amplified downstream magnetic field as expected for small scale turbulence. Electrons are accelerated to only 0.3 TeV, consistent with a diffusion coefficient many orders of magnitude greater than in the Bohm regime. If the same diffusion coefficient applies to the protons, their maximum energy is only ~100 TeV.Comment: Accepted for publication in ApJ. 6 pages - 2 figures. Minor correction

Analysis of Iterative Methods for the Steady and Unsteady Stokes Problem: Application to Spectral Element Discretizations

A new and detailed analysis of the basic Uzawa algorithm for decoupling of the pressure and the velocity in the steady and unsteady Stokes operator is presented. The paper focuses on the following new aspects: explicit construction of the Uzawa pressure-operator spectrum for a semiperiodic model problem; general relationship of the convergence rate of the Uzawa procedure to classical inf-sup discretization analysis; and application of the method to high-order variational discretization

Stability of Standing Accretion Shocks, With an Eye Toward Core Collapse Supernovae

We examine the stability of standing, spherical accretion shocks. Accretion shocks arise in core collapse supernovae (the focus of this paper), star formation, and accreting white dwarfs and neutron stars. We present a simple analytic model and use time-dependent hydrodynamics simulations to show that this solution is stable to radial perturbations. In two dimensions we show that small perturbations to a spherical shock front can lead to rapid growth of turbulence behind the shock, driven by the injection of vorticity from the now non-spherical shock. We discuss the ramifications this instability may have for the supernova mechanism.Comment: 21 pages, 13 figures; to be published in The Astrophysical Journa

Unconditional security proof of long-distance continuous-variable quantum key distribution with discrete modulation

We present a continuous-variable quantum key distribution protocol combining a discrete modulation and reverse reconciliation. This protocol is proven unconditionally secure and allows the distribution of secret keys over long distances, thanks to a reverse reconciliation scheme efficient at very low signal-to-noise ratio.Comment: 4 pages, 2 figure

Schwannomatosis of the Spinal Accessory Nerve: A Case Report.

Schwannomatosis is a distinct syndrome characterized by multiple peripheral nerve schwannomas that can be sporadic or familial in nature. Cases affecting the lower cranial nerves are infrequent. Here, the authors present a rare case of schwannomatosis affecting the left spinal accessory nerve. Upon genetic screening, an in-frame insertion at codon p.R177 of the Sox 10 gene was observed. There were no identifiable alterations in NF1, NF2, LZTR1, and SMARCB1. This case demonstrates a rare clinical presentation of schwannomatosis in addition to a genetic aberration that has not been previously reported in this disease context

Push clocks: a new approach to charge-coupled devices clocking

A new approach to charge-coupled device clocking has been developed—dynamic push clocks. With dynamic push clocks, the charge is transferred by pushing it from one storage site to another. The push clock approach results in a larger signal dynamic range, larger signal-to-noise ratio, and better performance at both high and low frequencies

Cosmic ray acceleration to ultrahigh energy in radio galaxies

The origin of ultrahigh energy cosmic rays (UHECRs) is an open question. In this proceeding, we first review the general physical requirements that a source must meet for acceleration to 10-100 EeV, including the consideration that the shock is not highly relativistic. We show that shocks in the backflows of radio galaxies can meet these requirements. We discuss a model in which giant-lobed radio galaxies such as Centaurus A and Fornax A act as slowly-leaking UHECR reservoirs, with the UHECRs being accelerated during a more powerful past episode. We also show that Centaurus A, Fornax A and other radio galaxies may explain the observed anisotropies in data from the Pierre Auger Observatory, before examining some of the difficulties in associating UHECR anisotropies with astrophysical sources.Comment: 6 pages, 4 figures. Proceedings of UHECR 2018, 8-12 October 2018, Paris, Franc