Sequential injection flow system with improved sample throughput: determination of glycerol and ethanol in wines

The performance of sequential injection (SI) systems has often been criticized for its low sampling frequency. The present work describes a SI system where an injection valve and an additional pump were incorporated to enhance sample throughput rate. The proposed systemwas applied to the enzymatic determination of glycerol and ethanol in wines, using spectrophotometric detection and immobilized glycerol and alcohol dehydrogenases. The method proposed was applied to the determination of ethanol between 0.10 and 0.50% (v/v) and glycerol between 0.03 and 0.30 g l−1. These ranges were appropriate for determination in table and port wines, since samples were diluted 50 times before introduction into the system. The results obtained from 15 wine samples were statistically comparable to those obtained by the reference methods, with good repeatability (R.S.D. < 3.4%, n = 10). The sampling rate was 22.5 h−1, corresponding to 45 determinations per hour. This way, the time required for each determination was decreased by 30% when compared to a conventional SI system

A review on sequential injection methods for water analysis

The development of fast, automatic and less expensive methods of analysis has always been the main aim of flowmethodologies. The search for newprocedures that still maintain the reliability and accuracy of the reference procedures is an ever growing challenge. Newrequirements are continually added to analytical methodologies, such as lower consumption of samples and reagents, miniaturisation and portability of the equipment, computer interfaces for full decision systems and so on. Therefore, the development of flow methodologies meeting the extra requirements of water analysis is a challenging work. Sequential injection analysis (SIA) presents a set of characteristics that make it highly suitable for water analysis. With sequential injection analysis, most routine determinations in waters can be performed more quickly with much lower reagent consumption when compared to reference procedures. Additionally, SIA can be a valuable tool for analyte speciation and multiparametric analysis. This paper critically reviews the overall work in this area.info:eu-repo/semantics/acceptedVersio

Constructed wetland systems vegetated with different plants applied to the treatment of tannery wastewater

Wastewaters from leather processing are very complex and lead to water pollution if discharged untreated, especially due to its high organic loading. In this study the survival of different plant species in subsurface horizontal flow constructed wetlands receiving tannery wastewater was investigated. Five pilot units were vegetated with Canna indica, Typha latifolia, Phragmites australis, Stenotaphrum secundatum and Iris pseudacorus, and a sixth unit was left as an unvegetated control. The treatment performance of the systems under two different hydraulic loading rates, 3 and 6 cmd1, was assessed. COD was reduced by 41–73% for an inlet organic loading varying between 332 and 1602 kg ha1 d1 and BOD5 was reduced by 41–58% for an inlet organic loading varying between 218 and 780 kg ha 1 d1. Nutrient removal occurred to lower extents. Phragmites australis and Typha latifolia were the only plants that were able to establish successfully. Despite the high removal of organic content from the influent wastewater, during 17 months of operation, no significant differences in performance were observed between units

Sequential injection titration of chloride in milk with potentiometric detection

In this work, a sequential injection system for the pseudo-titration of chloride in milk is described. Milk was directly aspirated into the system and sandwiched between two silver nitrate plugs (titrant). The aspirated zones were then propelled to the detector (Ag2S/Ag tubular electrode), where the depletion in the titrant silver concentration (due to the formation of a AgCl precipitate) was monitored. The results obtained by the developed sequential injection titration method were not statistically different from those provided by the potentiometric reference procedure with relative standard deviations better than 3.4%. The sampling rates achieved were 17 samples per hour.info:eu-repo/semantics/acceptedVersio

Treatment of industrial wastewater with two-stage constructed wetlands planted with typha latifolia and phragmites australis

Industrial wastewater treatment comprises several processes to fulfill the discharge permits or to enable the reuse of wastewater. For tannery wastewater, constructed wetlands (CWs) may be an interesting treatment option. Two-stage series of horizontal subsurface flow CWs with Phragmites australis (UP series) and Typha latifolia (UT series) provided high removal of organics from tannery wastewater, up to 88% of biochemical oxygen demand (BOD5) (from an inlet of 420 to 1000 mg L-1) and 92% of chemical oxygen demand (COD) (from an inlet of 808 to 2449 mg L-1), and of other contaminants, such as nitrogen, operating at hydraulic retention times of 2, 5 and 7 days. No significant (P < 0.05) differences in performance were found between both the series. Overall mass removals of up to 1294 kg COD ha-1 d-1 and 529 kg BOD5 h-1 d-1 were achieved for a loading ranging from 242 to 1925 kg COD ha-1 d-1 and from 126 to 900 kg BOD5 ha-1 d-1. Plants were resilient to the conditions imposed, however P. australis exceeded T. latifolia in terms of propagation.info:eu-repo/semantics/acceptedVersio

Flow injection system with gas diffusion for the sequential determination of total nitrogen and phosphorus in vegetables

A flow injection system was developed for the sequential determination of total nitrogen and phosphorus in digests of vegetables using potentiometric and spectrophotometric detection systems, respectively. A tubular ammonium selective electrode with a sensor system composed of nonactin/monactin in tris(2-ethylhexyl) phosphate was used. The selectivity limitations of this electrode were overcome by the inclusion of a gas-diffusion unit in the system that separated ammonium from the rest of the sample matrix and allowed the determination of total nitrogen and phosphorus by the partition of the sample plug between two streams. The results obtained with the developed FIA system were in good agreement with those of the reference methods. Sampling rates from 40 to 60 samples per hour and relative standard deviations below 3.5% were achieved

The application of multicommutated flow techniques to the determination of iron

New flow techniques such as multicommutated flow-injection analysis (MCFIA), multi-syringe FIA (MSFIA) and multi-pumping flow systems (MPFSs) have been developed. The low reagent consumption achieved by these techniques should be highlighted, as they can be regarded as environmentally friendly alternatives to conventional FIA. We present several applications of these novel flow techniques to the determination of iron.info:eu-repo/semantics/acceptedVersio

A flow system for the spectrophotometric determination of lead in different types of waters using ion-exchange for pre-concentration and elimination of interferences

A flow system for the spectrophotometric determination of lead in natural and waste waters is proposed. The determination is based on the colorimetric reaction between malachite green and iodide, followed by the formation of a ternary complex between those reagents and lead cations. The developed flow system includes a lead pre-concentration step in a column packed with a cationic resin (Chelex 100) operating in a sequential injection mode. To improve the mixture of sample and reagents, a flow injection approach was adopted for the colorimetric determination. This way a hybrid flow system, involving both sequential and flow injection concepts was designed. Another feature of the proposed system is the efficient elimination of major interferent species, such as cadmium and copper. The elimination of cadmium interference is obtained by complexing Cd2+ with chloride and retaining the formed negatively charged complexes in an anionic resin, AG1 X-8. As for copper, with the presence of both ionic resins as well as the conditions for cadmium elimination, it no longer acts as an interferent. Different ranges of lead concentration (50–300 and 300–1000 g l−1) can be determined with minor changes in the controlling software, useful for application to both natural and waste waters. Therefore, a detection limit of 25 g l−1 was achieved. Repeatability was evaluated from 10 consecutive determinations being the results better than 4%. The recoveries of lead spikes added to the samples ranged from 93 to 102%. The sampling frequency was 17 and 24 determinations per hour, for 50–300 and 300–1000 g l−1 ranges, respectively