Cultivating compassion through analysis of online patient narratives

Photos of male genitalia of Cx. univittatus from South Africa and Portuguese specimens. (PDF 3361 kb

Capture of biotin-gold nanoparticles by ZZ-CBM fusions in wax-printed microfluidic paper-based devices

<div><div><p>This video shows the capture of biotin-gold nanoparticles (40 nm) by a supramolecular complex in a paper-based microfluidic device. The microchannel structure (5 cm long) of the device is defined by wax-printing on chromatographic paper (Whatman N. 1). The supramolecular complex consists of 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module (CBM) with the ZZ fragment of the staphylococcal protein A and an anti-biotin antibody. This complex is pre-formed and pre-deposited in the circular regions of the device labeled T. The video shows the addition of biotin-gold nanoparticles at the leftmost region of the devices and subsequent migration through the channels by capillarity. As the fluid pass through the circular test zone, the capture of the gold nanoparticles by the ZZ-CBM:IgG complex becomes evident by the formation of a red spot. As fluid migration proceeds, the red signal increases in intensity and progressively extends to the center of the test zone. A polyester adhesive film is used to cover the back side of the second device and the back and front side of the third device. The video is accelerated approximately 20 times.</p></div><div><br></div></div

Gold nanoparticles anchored on cellulose microparticles by biomolecular recognition

Scanning electron microscopy analysis of the distribution of 40 nm biotin-gold nanoparticles immobilized on cellulose microparticles (Sigmacell 20) modified with a complex of 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module (CBM) with the ZZ fragment of the staphylococcal protein A and an anti-biotin antibody. Scale bar is 100 nm

Gold nanoparticles anchored on cellulose by biomolecular recognition

Scanning electron microscopy analysis of the distribution of 40 nm biotin-gold nanoparticles immobilized on chromatographic paper (Whatman N. 1) modified with a complex of 2 recognition elements: a ZZ-CBM3 fusion that combines a carbohydrate-binding module (CBM) with the ZZ fragment of the staphylococcal protein A and an anti-biotin antibody. Nanoparticle density is approximately 100 AuNPs per square micrometer. Scale bar is 100 nm

Additional file 1: Table S1. of Limited genomic divergence between intraspecific forms of Culex pipiens under different ecological pressures

Primers used in the AFLP protocol. Table S2 Error rates of the loci obtained by each primer combination in the selective amplification. Table S3. Population diversity of the eight populations used in the study. Table S4. Divergence estimates based on FST pairwise sample analysis per locus within pipiens and molestus samples. Table S5. Divergence estimates based in FST pairwise sample analysis per locus between pipiens and molestus samples. Table S6. Loci detected as outliers in each comparative analysis (Europe and USA). Table S7. Proportion of the loci by fragment size in the Overall and USA data. Fig. S1. Graphics of ad hoc approaches to infer the number of clusters (K) in STRUCTURE analysis with all samples. Fig. S2. Outlier detection results from MCHEZA analyses. (PDF 451 kb