Estimate or the effectiveness of intelligent information system of early diagnosis and prognosis of cardiovascular disease

This article describes a new approach to the implementation of the training program of risk probability of coronary heart disease (CHD) remote evaluation. It describes an example of training sample formation based on the statistical analysis of medical data. The proposed solution will allow medical personnel to carry out the personification of cardiac services and first of all, preventive orientation. Also a method for assessing the effectiveness of the program of expert system "ARM-Cardiologist" on the results of ongoing clinical trials is providedyesБелгородский государственный университе

Increased Expression of Clusterin in Kidney of a Fatal Nephropathia Epidemica Case

© 2016, Springer Science+Business Media New York.Nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS), is endemic in the European part of Russia. This disease is characterized by an acute onset followed by symptoms related to kidney impairment. Histologically, NE is defined as a tubulointerstitial nephritis with the prominent leukocyte infiltration and interstitial hemorrhages. Also, the presence of IgM, complement component C3, and fibrin deposits along the basal surface of the tubular epithelial cells has been reported. The pathogenesis of immunoglobulin and complement deposition in the kidney tubules remains unknown. We found clusterin deposits in the kidney tissue from fatal NE. Clusterin was co-localized with hantavirus antigens and C3 complement on the basal side of the kidney tubules. Additionally, kidney infiltrating leukocytes were positive for clusterin. It has previously been documented that the expression of clusterin is restricted to epithelial cells and is often found on tubular cells of the injured kidney tissue. Additionally, clusterin can bind to immunoglobulins and complement components. We propose that upregulation of clusterin expression in the NE kidney is associated with damage to the tissue and may cause deposition of immunoglobulins and complement

Biosafety model of adenovirus infection: Effects of bacterial proteases for infection of human cells in vitro

To determine the antiviral activity of various biologically active compounds, the model of adenovirus infection on the basis of cell cultures of human HEK293A and recombinant adenovirus Ad-EGFP, expressing green fluorescent protein EGFP. Adenoviruses have a capsid size of 70-90 nm and are able to infect dividing and nondividing cells in vitro and in vivo. Recombinant adenoviruses are the replicative defect in the cells of humans and animals. The developed model allowed us to determine the effect of bacterial proteases in the infected cell cultures with adenovirus. This model can also be used for screening drugs with potential protivivovirusnoy activity

Experimental and clinical approaches to a radioimmunotherapy in o

Molecular nuclear medicine plays an important role in the diagnosis and therapy of cancer. Radioimmunodetection and radioimmunotherapy (RIT) involve the use of antibodies conjugated with diagnostic or therapeutic radionuclides, respectively. More often for RIT use the monoclonal antibodies against tumor-associated antigens, radiolabeled with alpha or beta-emitters. Encouraging results have been achieved with this technology in the management of hematologic malignancies. On the contrary, solid tumors have been less responsive. Radioimmunotherapy therefore is considered more suitable for the treatment of microscopic or minimal residual disease, allowing radiolabeled monoclonal antibodies to achieve uptake in tumors high enough to result in tumoricidal radiation doses. Despite these encouraging results, new potential target for radioimmunodetection and RIT should be found. For this purpose, a series of experiments will be performing to investigate the biodistribution and the therapeutic efficacy of radiolabeled monoclonal antibodies in cell culture and animal models

Increased Expression of Clusterin in Kidney of a Fatal Nephropathia Epidemica Case

© 2016, Springer Science+Business Media New York.Nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS), is endemic in the European part of Russia. This disease is characterized by an acute onset followed by symptoms related to kidney impairment. Histologically, NE is defined as a tubulointerstitial nephritis with the prominent leukocyte infiltration and interstitial hemorrhages. Also, the presence of IgM, complement component C3, and fibrin deposits along the basal surface of the tubular epithelial cells has been reported. The pathogenesis of immunoglobulin and complement deposition in the kidney tubules remains unknown. We found clusterin deposits in the kidney tissue from fatal NE. Clusterin was co-localized with hantavirus antigens and C3 complement on the basal side of the kidney tubules. Additionally, kidney infiltrating leukocytes were positive for clusterin. It has previously been documented that the expression of clusterin is restricted to epithelial cells and is often found on tubular cells of the injured kidney tissue. Additionally, clusterin can bind to immunoglobulins and complement components. We propose that upregulation of clusterin expression in the NE kidney is associated with damage to the tissue and may cause deposition of immunoglobulins and complement

Increased Expression of Clusterin in Kidney of a Fatal Nephropathia Epidemica Case

© 2016, Springer Science+Business Media New York.Nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS), is endemic in the European part of Russia. This disease is characterized by an acute onset followed by symptoms related to kidney impairment. Histologically, NE is defined as a tubulointerstitial nephritis with the prominent leukocyte infiltration and interstitial hemorrhages. Also, the presence of IgM, complement component C3, and fibrin deposits along the basal surface of the tubular epithelial cells has been reported. The pathogenesis of immunoglobulin and complement deposition in the kidney tubules remains unknown. We found clusterin deposits in the kidney tissue from fatal NE. Clusterin was co-localized with hantavirus antigens and C3 complement on the basal side of the kidney tubules. Additionally, kidney infiltrating leukocytes were positive for clusterin. It has previously been documented that the expression of clusterin is restricted to epithelial cells and is often found on tubular cells of the injured kidney tissue. Additionally, clusterin can bind to immunoglobulins and complement components. We propose that upregulation of clusterin expression in the NE kidney is associated with damage to the tissue and may cause deposition of immunoglobulins and complement

Increased Expression of Clusterin in Kidney of a Fatal Nephropathia Epidemica Case

© 2016, Springer Science+Business Media New York.Nephropathia epidemica (NE), a mild form of hemorrhagic fever with renal syndrome (HFRS), is endemic in the European part of Russia. This disease is characterized by an acute onset followed by symptoms related to kidney impairment. Histologically, NE is defined as a tubulointerstitial nephritis with the prominent leukocyte infiltration and interstitial hemorrhages. Also, the presence of IgM, complement component C3, and fibrin deposits along the basal surface of the tubular epithelial cells has been reported. The pathogenesis of immunoglobulin and complement deposition in the kidney tubules remains unknown. We found clusterin deposits in the kidney tissue from fatal NE. Clusterin was co-localized with hantavirus antigens and C3 complement on the basal side of the kidney tubules. Additionally, kidney infiltrating leukocytes were positive for clusterin. It has previously been documented that the expression of clusterin is restricted to epithelial cells and is often found on tubular cells of the injured kidney tissue. Additionally, clusterin can bind to immunoglobulins and complement components. We propose that upregulation of clusterin expression in the NE kidney is associated with damage to the tissue and may cause deposition of immunoglobulins and complement

Biosafety model of adenovirus infection: Effects of bacterial proteases for infection of human cells in vitro

To determine the antiviral activity of various biologically active compounds, the model of adenovirus infection on the basis of cell cultures of human HEK293A and recombinant adenovirus Ad-EGFP, expressing green fluorescent protein EGFP. Adenoviruses have a capsid size of 70-90 nm and are able to infect dividing and nondividing cells in vitro and in vivo. Recombinant adenoviruses are the replicative defect in the cells of humans and animals. The developed model allowed us to determine the effect of bacterial proteases in the infected cell cultures with adenovirus. This model can also be used for screening drugs with potential protivivovirusnoy activity