An Oral Recombinant Vaccine in Dogs against Echinococcus granulosus, the Causative Agent of Human Hydatid Disease: A Pilot Study

Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium

LES REPTILES (THESE PRESENTEE SUR SUPPORT INFORMATIQUE)

LYON1-BU Santé (693882101) / SudocSudocFranceF

Immunocompetent Cell Responses at the Level of the Duodenum of Vaccinated and Control Dogs.

<p>(A) Duodenum of a vaccinated dog: The interstitial infiltrate of immunocompetent cells are labelled with a polyclonal rabbit antiserum specific for the Fc fragment from IgA of dog. IgA. Binding is visualized by the peroxidase reaction (brown cells) and hematoxylin counter stained. (B) Duodenum of an infected control dog: The interstitial infiltrate of immunocompetent cells are labelled with a polyclonal rabbit antiserum specific for the Fc fragment from IgA of dog. IgA. Binding is visualized by the peroxidase reaction (brown cells) and hematoxylin counter stained. (C) Duodenum of a vaccinated dog: T cells in the interstitium at the top of the villi are immunolabelled with rat antihuman CD3IgG1, clone number CD3-12. Binding is visualized by the peroxidase reaction (brown cells) and hematoxylin counterstained. (D) Duodenum of a control infected dog: T cells in the interstitium at the top of the villi are immunolabelled with rat antihuman CD3IgG1, clone number CD3-12. Binding is visualized by the peroxidase reaction (brown cells) and hematoxylin counterstained. (E) Duodenum of a vaccinated dog: on the right: the base of the villus: a goblet cell (GC) is included in the epithelium. Brush border (BB) is present at the apex of epithelial cells (EC); On the left: the subjacent interstitium is invaded by an immunocompetent cell infiltrate: plasma cells (PC) are numerous. LC, lymphocyte. (F) Duodenum of a control infected dog: on the right epithelial cells (EC) from the villus then basal lamina (BL). On the left the subjacent interstitium shows the presence of fibroblast (FC) and immunocompetent cells: plasma cell (Pl) and polynuclear cell (PN).</p

Number of Positive Cells per Square Millimeter after Immunostaining with Various Antibodies in the Villous Lamina Propria of the Duodenum

<p>The average of Lambda positive cells was 1,594 per mm² and of Kappa positive cells 322 per mm²; thus the λ/κ ratio is 5. The average of IgA positive cells was 1,335 per mm² and of IgM positive cells 202 per mm², thus the IgA/IgM ratio is 6.6.</p>*<p><i>P</i><0.05, Dunnett multiple comparisons test.</p>##<p><i>P</i><0.01, Dunnett multiple comparisons test.</p>#<p><i>P</i><0.05, Dunnett multiple comparisons test.</p>#<p><i>P</i>-value as compared with dogs who received only <i>Salmonella</i>.</p>**<p><i>P</i>-value as compared with dogs from control groups.</p

Age, Sex, and Status of the Group of Dogs Used in the Experiments in Morocco and Tunisia

<p>Age, Sex, and Status of the Group of Dogs Used in the Experiments in Morocco and Tunisia</p