Cell Membrane Lipid Composition in Relation to Physical Properties and Function: A Methodological Study

Abnormalities on membrane function are widespread in many diseased states. It has been for some time postulated that these abnormalities are not due to the membrane proteins themselves but the matrix surrounding them; the phospholipid bilayer. The composition of the membrane affects its fluidity, which in turn affects its function. This composition is complex and although methods have been published which examine some aspects, few have succeed in providing a comprehensive analysis. This was the principle aim of the current study

Dietary potassium supplementation improves vascular structure and ameliorates the damage caused by cerebral ischemia in normotensive rats

<p>Abstract</p> <p>Background</p> <p>Dietary potassium supplementation in hypertensive rats is cardioprotective. This protection includes a blood pressure independent reduction in the amount of damage caused by cerebral ischemia. Therefore, we hypothesized that dietary potassium supplementation would improve the outcome of ischemic stroke by improving cerebral vessel structure in normotensive rats.</p> <p>Methods</p> <p>Wistar Kyoto (WKY) rats were fed a high (HK) or low potassium (LK) diet for six weeks from six weeks of age. At the end of treatment, cerebral ischemia was induced by middle cerebral artery (MCA) occlusion and the resultant infarct was quantified and expressed as a percentage of the hemisphere infarcted (%HI). MCA structure was assessed in an additional group of rats using a pressurized arteriograph.</p> <p>Results</p> <p>The cerebral infarct was significantly smaller in rats fed the HK diet, compared to rats fed the LK diet (21 ± 5.4 vs 33.5 ± 4.8 %HI HK vs LK p < 0.05). Vessel structure was improved in WKY rats fed the HK diet as indicated by an increase in the MCA lumen (298 ± 6.3 vs 276 ± 3.9 μm HK vs LK p < 0.05) and outer diameters (322 ± 7.6 vs 305 ± 4.8 μm HK vs LK p < 0.05). Wall thickness and area were unchanged, suggesting an outward euthrophic remodelling process. The HK diet had no effect on body weight or telemetry blood pressure.</p> <p>Conclusion</p> <p>These studies are the first to show a beneficial effect of dietary potassium in rats with normal blood pressure.</p

Managing Soybean Rustin South Dakota in 2006

Soybean rust, caused by the fungus Phakopsora pachyrhizi, was first introduced to the U.S. in Fall 2004. It survived the winter in small pockets of infected kudzu in Florida and produced a widespread low-severity disease in the southeastern U.S. in 2005. During Winter 2005, the pathogen survived over a much wider area and appears to have become established in Mexico. That means the threat from soybean rust is greater in 2006 than in 2005, and we can expect that the threat will generally increase over the coming years as the pathogen becomes established in new areas with little risk of winter freeze. Fungicides remain the only practical and effective control for this disease. Several fungicides have received EPA labeling and more products are being considered. Which fungicide products are available is a constantly changing landscape. Check with your local county Extension office for a listing of the most current products or go to http://plantsci.sdstate.edu/planthealth and link to Soybean Rust

Phytophthora root and stem rot of soybean

Phytophthora sojae is a soil borne pathogen that in the past has caused very large economic losses. During the late 1970s, 300,000 soybean acres (approximately 10% of total soybean production in Ohio) were lost due to P. sojae. This disease has since been effectively managed predominately through the incorporation of single-gene mediated resistance but quantitative or partial resistance has been used as well. In fact, today, we can repeat 100% loss by planting soybean cultivars that were popular during earlier epidemics. Without high levels of resistance to this pathogen, many soybean acres would be lost each year to this disease. Phytophthora doesn’t forget and it doesn’t go away

Comparison of three microsatellite analysis methods for detecting genetic diversity in Phytophthora sojae (Stramenopila: Oomycete)

Analysis of an organism’s genetic diversity requires a method that gives reliable, reproducible results. Microsatellites are robust markers, however, detection of allele sizes can be difficult with some systems as well as consistency among laboratories. In this study, our two laboratories used 219 isolates of Phytophthora sojae to compare three microsatellite methods. Two capillary electrophoresis methods, the Applied Biosystems 3730 Genetic Analyzer and the CEQ 8000 Genetic Analysis system, detected an average of 2.4-fold more alleles compared to gel electrophoresis with a mean of 8.8 and 3.6 alleles per locus using capillary and gel methods, respectively. The two capillary methods were comparable, although allele sizes differed consistently by an average of 3.2 bp across isolates. Differences between capillary methods could be overcome if reference standard DNA genotypes are shared between collaborating laboratories

Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

Major quantitative disease resistance loci (QDRLs) are rare in the Phytophthora sojae (Kaufmann and Gerdemann)–soybean [Glycine max (L). Merr.] pathosystem. A major QDRL on chromosome 18 (QDRL-18) was identified in PI 427105B and PI 427106. QDRL-18 represents a valuable resistance source for breeding programs. Thus, our objectives were to determine its isolate specificity and measure its effect on yield and resistance to both P. sojae and other soybean pathogens. We characterized near isogenic lines (NILs) developed from F7 recombinant inbred lines heterozygous at QDRL-18; NILs represent introgressions from PI 427105B, PI 427106, and susceptible ‘OX20- 8’. The introgressions from PI 427105B and PI 427106 increased resistance to P. sojae by 11 to 20% and 35 to 40%, respectively, based on laboratory and greenhouse assays, and increased yield by 13 to 29% under disease conditions. The resistant introgression from PI 427105B was also effective against seven P. sojae isolates with no isolate specificity detected. Based on quantitative polymerase chain reaction assays, NILs with the susceptible introgression had significantly higher relative levels of P. sojae colonization 48 h after inoculation. No pleiotropic effects for resistance to either soybean cyst nematode or Fusarium graminearum were detected. This information improves soybean breeders’ ability to make informed decisions regarding the deployment of QDRL-18 in their respective breeding programs

An Immunofluorescence Assay to Detect Urediniospores of \u3ci\u3ePhakopsora pachyrhizi\u3c/i\u3e

An indirect immunofluorescence spore assay (IFSA) was developed to detect urediniospores of Phakopsora pachyrhizi, utilizing rabbit polyclonal antisera produced in response to intact nongerminated (SBR1A) or germinated (SBR2) urediniospores of P. pachyrhizi. Both antisera were specific to Phakopsora spp. and did not react with other common soybean pathogens or healthy soybean leaf tissue in enzyme-linked immunosorbent assay (ELISA). SBR1A and SBR2 bound to P. pachyrhizi and P. meibomiae urediniospores were detected with goat anti-rabbit Alexa Fluor 488-tagged antiserum using a Leica DM IRB epifluorescent microscope with an I3 blue filter (excitation 450 to 490 nm, emission 515 nm). The assay was performed on standard glass microscope slides; double-sided tape was superior to a thin coating of petroleum jelly both in retaining spores and in immunofluorescence. The IFSA was used to confirm the identity of P. pachyrhizi urediniospores captured on glass slides from passive air samplers from Georgia, Kentucky, and Ohio during 2006