5 research outputs found

    Additional file 7: Figure S6. of Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images

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    Benchmarking of cell row counter accuracy for C. elegans gonadal arms. (A) Overlay of microscope image (white signal, derived from the DNA stain DAPI) with segmentations color-coded by cell row position from the distal end (left), as computed automatically using our counter. (B) Size of the MZ scored manually vs size computed through the automatic counter. A small amount of noise (0.5 cell rows) was added in order to aid visualization of overlapping data. Positions scored manually and automatically are in close agreement; average percent deviation is 9.4脗聽%. Diagonal shown for reference in red. (PDF 152脗聽kb

    Additional file 10: Figure S8. of Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images

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    Comparison of frozen or vibratome section segmentations. (A) Active contour segmentation of frozen sections is often inaccurate (red arrows) due to poor cell separation in the original image. (B) Vibratome sections enable clearer staining and thus more accurate segmentation (yellow arrows). (PDF 749脗聽kb
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