Shyness, Verbal Irony Comprehension, and Socio-Emotional Functioning in Middle Childhood

Shy children simultaneously desire to engage in social interactions with their peers and to avoid social situations (particularly novel ones) due to feelings of anxiety and self-consciousness. Although not in itself considered pathological, childhood shyness is a significant risk factor for a number of negative social and emotional outcomes including peer victimization, low perceived friendship quality, loneliness, and symptoms of depression. Previous work finds that pragmatic language skills and aspects of communicative competence influence the relationship between shyness and these negative outcomes. Counterfactual verbal irony, in which the literal meaning of an utterance is directly opposite its intended meaning, is a figurative language form that has been posited to have a number of important social functions. The Tinge Hypothesis, for example, claims that the use of verbal irony mutes the critical tone of ironic criticisms, and simultaneously renders ironic compliments less kind than literal compliments. It has also been shown that shyness is related to children’s ratings of the attitude of ironic speakers, with children who are shyer rating ironic criticisms as being meaner than did less shy children. Building on these findings, and the potential protective role of good communication skills, this dissertation examined relations between verbal irony comprehension, effective communication, and socio-emotional outcomes in shy children aged 8-12 years. Using a series of vignettes and self-report measures, Study 1 revealed that while neither verbal irony comprehension nor socio-communicative skills mediated the relationship between shyness and its associated negative outcomes, verbal irony comprehension moderated the relationship between shyness and symptoms of loneliness and depression. Contrary to predictions, shy children with better verbal irony comprehension had greater loneliness and depression symptoms. Similarly, for girls, better verbal irony comprehension strengthened the relationship between shyness and peer victimization. In contrast, for boys, better verbal irony comprehension and better socio-communicative skills were associated with a reduction in the risk of victimization associated with shyness. Possible explanations for these unexpected findings are discussed. Within the broad aim of examining the interplay between trait shyness and ironic language use, I was also interested in whether shyness influenced children’s perceptions of the communicative intentions and personal characteristics of ironic speakers beyond judging speaker attitudes. In Study 2 children were asked to rate fictional characters on a number of dimensions after reading short vignettes in which the characters interacted with a same-gendered peer using sarcastic or literal criticism or praise. Children generally rated speakers who used verbal irony as being funnier, yet less kind than speakers who made literal remarks. Non-shy participants also indicated that they would be less likely to befriend a speaker who used irony with a shy target, suggesting that non-shy children may feel it is less appropriate to use verbal irony with shy children. Overall, regardless of whether speakers used ironic or literal statements, shy children felt that the speaker was kinder and more popular when they had seen the speaker interacting with a shy target, suggesting that the participants may have been identifying with the targets. Across the two studies, the majority of the findings were consistent with the Tinge Hypothesis, with children rating speakers who made ironic compliments less favourably than those who made literal compliments. In a second part of Study 2, when children were asked to imagine themselves in the vignettes and then indicate the likelihood that they, themselves, would tell the truth, lie, use sarcasm or make a prosocial remark, the findings again followed the Tinge Hypothesis, with sarcasm being favoured over literal criticisms, but literal compliments being favoured over ironic compliments. Girls’ responses were more consistent with this trend, while boys were more likely to choose the less prosocial options, such as literal criticisms, ironic compliments, and lying in positive contexts. These two studies explore the complex relations between shyness, gender, verbal irony comprehension, and social and emotional functioning (Study 1), and children’s perceptions of speakers using verbal irony (Study 2). Many of the findings suggested that for shy children strong verbal irony comprehension and socio-communicative competence were related to poorer social and emotional outcomes. This suggests that targeting skills deficits may not be the route to mitigating the negative social and emotional outcomes associated with shyness. Shyness was not related to perceptions to ironic speakers across many measures, although there was some preliminary evidence that non-shy children thought it was less appropriate to use irony with shy targets. Further research should continue to explore how the risks associated with childhood shyness could be mitigated

What Are You Really Saying? Verbal Irony Understanding in Children with Social Anxiety Symptoms and Shy Negative Affect

Verbal irony, a form of figurative language, uses the discrepancy between a speaker’s intended meaning and the literal word meanings to achieve social goals. Yet, little research exists on individual differences that may disrupt irony understanding. Verbal irony may challenge shy children, who tend to interpret ambiguous stimuli as being threatening, and who have difficulties with mentalizing in social contexts. This study assessed whether shy children interpret ironic statements differently than do non-shy children. Children (8- to11-year-olds) listened to stories wherein one character made a statement to another character that was a literal or ironic criticism or a literal or ironic compliment. Children appraised the speaker’s belief and communicative intention. Shyness was assessed using self report measures of social anxiety symptoms and shy negative affect. Shy children did not differ from non-shy peers in comprehending speakers’ beliefs. However, shy children rated speakers who made ironic criticisms as being more mean than did children low in shyness. Thus, while understanding that speakers intended to communicate their true beliefs, shy children construed the social meaning of irony differently, indicating difficulties with pragmatics. Such subtle differences in pragmatic understanding may underlie some of the social difficulties facing shy children

Quaranteens: Prepandemic relationship quality and changes in adolescent internalizing problems during the COVID-19 pandemic

This preregistered longitudinal study examined changes in adolescents' depressive and anxiety symptoms before and during the COVID-19 pandemic using latent additive piece-wise growth models. It also assessed whether support from and conflict with mothers, fathers, siblings, and best friends explained heterogeneity in change patterns. One hundred and ninety-two Dutch adolescents (Mean age: 14.3 years; 68.8% female) completed online biweekly questionnaires for a year (November 2019–October 2020), consisting of a prepandemic, lockdown, and reopening phase. Depressive symptoms increased following the lockdown and decreased upon reopening. Anxiety symptoms showed an immediate decrease followed by a gradual increase in the reopening phase. Prepandemic family and best friend support and conflict did not explain heterogeneity in depressive and anxiety symptoms during the COVID-19 pandemic.</p

An Antithrombin-Heparin Complex Increases the Anticoagulant Activity of Fibrin Clots

Clotting blood contains fibrin-bound thrombin, which is a major source of procoagulant activity leading to clot extension and further activation of coagulation. When bound to fibrin, thrombin is protected from inhibition by antithrombin (AT) + heparin but is neutralized when AT and heparin are covalently linked (ATH). Here, we report the surprising observation that, rather than yielding an inert complex, thrombin-ATH formation converts clots into anticoagulant surfaces that effectively catalyze inhibition of thrombin in the surrounding environment

Treating postnatal depressive symptoms in primary care: a randomised controlled trial of GP management, with and without adjunctive counselling

BACKGROUND: Postnatal depression (PND) is under-diagnosed and most women do not access effective help. We aimed to evaluate comparative management of (PND) following screening with the Edinburgh Postnatal Depression Scale, using three best-practice care pathways by comparing management by general practitioners (GPs) alone compared to adjunctive counselling, based on cognitive behavioural therapy (CBT), delivered by postnatal nurses or psychologists. METHODS: This was a parallel, three-group randomised controlled trial conducted in a primary care setting (general practices and maternal & child health centres) and a psychology clinic. A total of 3,531 postnatal women were screened for symptoms of depression; 333 scored above cut-off on the screening tool and 169 were referred to the study. Sixty-eight of these women were randomised between the three treatment groups. RESULTS: Mean scores on the Beck Depression Inventory (BDI-II) at entry were in the moderate-to-severe range. There was significant variation in the post-study frequency of scores exceeding the threshold indicative of mild-to-severe depressive symptoms, such that more women receiving only GP management remained above the cut-off score after treatment (p = .028). However, all three treatment conditions were accompanied by significant reductions in depressive symptoms and mean post-study BDI-II scores were similar between groups. Compliance was high in all three groups. Women rated the treatments as highly effective. Rates of both referral to the study (51%), and subsequent treatment uptake (40%) were low. CONCLUSIONS: Data from this small study suggest that GP management of PND when augmented by a CBT-counselling package may be successful in reducing depressive symptoms in more patients compared to GP management alone. The relatively low rates of referral and treatment uptake, suggest that help-seeking remains an issue for many women with PND, consistent with previous research

No effect of aspirin on mammographic density in a randomized controlled clinical trial.

BACKGROUND: Epidemiologic studies suggest a reduced risk of breast cancer among women who regularly use aspirin; a plausible mechanism is through aspirin effect on mammographic breast density, a breast cancer risk factor, possibly mediated through aspirin interference with estrogen synthesis. METHODS: In a 2-arm randomized placebo-controlled clinical trial, we evaluated the effects of 6-month administration of 325 mg/day aspirin on total mammographic breast dense area and percent of the mammographic breast image occupied by dense areas (% density) in 143 postmenopausal women. Eligible women, recruited from 2005 to 2007, were healthy, not taking hormone therapy, with elevated mammographic breast density (American College of Radiology Breast Imaging Reporting and Data System density category 2, 3, or 4) within 6 months before enrollment. RESULTS: Women were a mean (SD) 59.5 (5.5) years. Geometric mean baseline percent density was 17.6% (95% confidence interval, 14.8-20.9) in women randomized to aspirin and 19.2% (95% confidence interval, 16.3-22.7) in women randomized to placebo. Percent density decreased in women randomized to aspirin by an absolute 0.8% versus an absolute decrease of 1.2% in controls (P = 0.84). Total breast area and dense area decreased to a similar degree in women assigned to aspirin and in those assigned to placebo, with no statistically significant differences between trial arms. CONCLUSIONS: A single daily administration of adult-dose aspirin for 6 months had no effect on mammographic density in postmenopausal women. If aspirin affects breast cancer risk in postmenopausal women, it may do so through alternative pathways than mammographic breast density. (Cancer Epidemiol Biomarkers Prev 2009;18(5):1524-30)

Investigating food preference in zoo‐housed meerkats

Understanding food preference among animals in human care can support improvements to welfare through training and day‐to‐day care (e.g., diet management). Little has been published about food preference in zoo‐housed meerkats. Assessing meerkat food preference would be useful, not only for the welfare of that species, but also for developing approaches to assessing food preference in other grouphoused, social species. The specific aim of this study was to quantify food preference within the meerkat mob at Wellington Zoo. We developed a simple, cost‐effective method for characterizing the food preference hierarchy in meerkats by presenting pairs of foods to the mob as a group. We observed stable preference with the hierarchy for pups closely resembling that for adults. This study demonstrated that it is possible to assess food preference and identify a food preference hierarchy for a group of animals from a social species where it was neither practical nor appropriate to assess individuals' preferences separately

Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains

Staphylococcus aureus: with the sequence type (ST) 398 was previously associated with livestock carriage. However, in recent years livestock-independent S. aureus ST398 has emerged, representing a potential health risk for humans especially in nosocomial settings. Judged by whole-genome sequencing analyses, the livestock- and human originated strains belong to two different S. aureus ST398 clades but, to date, it was not known to what extent these clades differ in terms of actual virulence. Therefore, the objective of this study was to profile the exoproteomes of 30 representative S. aureus ST398 strains by mass spectrometry, to assess clade-specific differences in virulence factor secretion, and to correlate the identified proteins and their relative abundance to the strains' actual virulence. Although the human-originated strains are more heterogeneous at the genome level, our observations show that they are more homogeneous in terms of virulence factor production than the livestock-associated strains. To assess differences in virulence, infection models based on larvae of the wax moth Galleria mellonella and the human HeLa cell line were applied. Correlation of the exoproteome data to larval killing and toxicity toward HeLa cells uncovered critical roles of the staphylococcal Sbi, SpA, SCIN and CHIPS proteins in virulence. These findings were validated by showing that sbi or spa mutant bacteria are attenuated in G. mellonella and that the purified SCIN and CHIPS proteins are toxic for HeLa cells. Altogether, we show that exoproteome profiling allows the identification of critical determinants for virulence of livestock-associated and human-originated S. aureus ST398 strains

Quaranteens: Prepandemic relationship quality and changes in adolescent internalizing problems during the COVID-19 pandemic

This preregistered longitudinal study examined changes in adolescents' depressive and anxiety symptoms before and during the COVID-19 pandemic using latent additive piece-wise growth models. It also assessed whether support from and conflict with mothers, fathers, siblings, and best friends explained heterogeneity in change patterns. One hundred and ninety-two Dutch adolescents (Mean age: 14.3 years; 68.8% female) completed online biweekly questionnaires for a year (November 2019–October 2020), consisting of a prepandemic, lockdown, and reopening phase. Depressive symptoms increased following the lockdown and decreased upon reopening. Anxiety symptoms showed an immediate decrease followed by a gradual increase in the reopening phase. Prepandemic family and best friend support and conflict did not explain heterogeneity in depressive and anxiety symptoms during the COVID-19 pandemic

Connections between Exoproteome Heterogeneity and Virulence in the Oral Pathogen Aggregatibacter actinomycetemcomitans

Aggregatibacter actinomycetemcomitans is a Gram-negative bacterial pathogen associated with severe periodontitis and nonoral diseases. Clinical isolates of A. actinomycetemcomitans display a rough (R) colony phenotype with strong adherent properties. Upon prolonged culturing, nonadherent strains with a smooth (S) colony phenotype emerge. To date, most virulence studies on A. actinomycetemcomitans have been performed with S strains of A. actinomycetemcomitans, whereas the virulence of clinical R isolates has received relatively little attention. Since the extracellular proteome is the main bacterial reservoir of virulence factors, the present study was aimed at a comparative analysis of this subproteome fraction for a collection of R isolates and derivative S strains, in order to link particular proteins to the virulence of A. actinomycetemcomitans with serotype b. To assess the bacterial virulence, we applied different infection models based on larvae of the greater wax moth Galleria mellonella, a human salivary gland-derived epithelial cell line, and freshly isolated neutrophils from healthy human volunteers. A total number of 351 extracellular A. actinomycetemcomitans proteins was identified by mass spectrometry, with the S strains consistently showing more extracellular proteins than their parental R isolates. A total of 50 known extracellular virulence factors was identified, of which 15 were expressed by all investigated bacteria. Importantly, the comparison of differences in exoproteome composition and virulence highlights critical roles of 10 extracellular proteins in the different infection models. Together, our findings provide novel clues for understanding the virulence of A. actinomycetemcomitans and for development of potential preventive or therapeutic avenues to neutralize this important oral pathogen. IMPORTANCE Periodontitis is one of the most common inflammatory diseases worldwide, causing high morbidity and decreasing the quality of life of millions of people. The bacterial pathogen Aggregatibacter actinomycetemcomitans is strongly associated with aggressive forms of periodontitis. Moreover, it has been implicated in serious nonoral infections, including endocarditis and brain abscesses. Therefore, it is important to investigate how A. actinomycetemcomitans can cause disease. In the present study, we applied a mass spectrometry approach to make an inventory of the virulence factors secreted by different clinical A. actinomycetemcomitans isolates and derivative strains that emerged upon culturing. We subsequently correlated the secreted virulence factors to the pathogenicity of the investigated bacteria in different infection models. The results show that a limited number of extracellular virulence factors of A. actinomycetemcomitans have central roles in pathogenesis, indicating that they could be druggable targets to prevent or treat oral disease