Steady state tuber concentration of sucrose and glucose phosphates.

<p>A. Sucrose concentrations A: Example chromatogram indicating G1P and G6P peaks. B: G1P/G6P ratios. C: G1P concentrations. D: G6P concentrations. Error bars indicate standard error of measurement. The asterisk indicates metabolite levels significantly different for Kuras versus Desiree and Jutlandia (p-value<0.05). The determination of G1P/G6P is much more accurate than individual determination of G1P and G6P (see text for detail).</p

Sample statistics.

<p>Total number of DeepSAGE tags determined for each library for Desiree, Jutlandia and Kuras cultivars. Data are represented for all three biological replicates for each time point: 9, 12, 15, 18 and 21 weeks after planting (2008) and 9 and 11 weeks after planting (2009). Combined tag counts of biological replicates are also presented. The last columns represent the tuber yield in hkg/ha and starch content of Jutlandia, Desiree and Kuras cultivars.</p

Potato tuber gene expression in starch metabolism throughout tuber bulking.

<p>SAGE tags were mapped to genes encoding enzymes involved in starch metabolism. Tag counts per million (y-axis) for multiple enzyme isoforms catalyzing a single step were summed. Desiree is shown in red, Jutlandia in blue and Kuras in green. The error bars indicate standard error of measurement. Numbers on x-axis indicate weeks after planting. Left side of the hatched bars: 2008; right side: 2009. The asterisk symbol indicates gene expression values significantly different than the gene expression of the two remaining cultivars (p-value<0.05). The solid reaction arrows indicate the major metabolic route of high carbon flux leading to starch accumulation. The hatched lines indicate minor metabolic routes where carbon flux is limited. The red hatched box indicates the G1P transporter suggested by Fettke and coworkers and discussed in this study. The green solid line indicate cell membrane, the yellow line indicates the amyloplastic membrane.</p