2 research outputs found
Bioinstructive Coatings for Hematopoietic Stem Cell Expansion Based on Chemical Vapor Deposition Copolymerization
We
report the chemical vapor deposition (CVD) of dual-functional
polymer films for the specific and orthogonal immobilization of two
biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide)
that govern the fate of hematopoietic stem and progenitor cells. The
composition of the CVD polymer and thus the biomolecule ratio can
be tailored to investigate and optimize the influence of the relative
surface concentrations of biomolecules on stem cell behavior. Prior
to cell experiments, all surfaces were characterized by infrared reflection
adsorption spectroscopy, time-of-flight secondary ion mass spectrometry,
and X-ray photoelectron spectroscopy to confirm the presence of both
biomolecules. In a proof-of-principle stem cell culture study, we
show that all polymer surfaces are cytocompatible and that the proliferation
of the hematopoietic stem and progenitor cells is predominantly influenced
by the surface concentration of immobilized DLL1
Hierarchically Functionalized Magnetic Core/Multishell Particles and Their Postsynthetic Conversion to Polymer Capsules
The controlled synthesis of hierarchically functionalized core/multishell particles is highly desirable for applications in medicine, catalysis, and separation. Here, we describe the synthesis of hierarchically structured metal–organic framework multishells around magnetic core particles (magMOFs) <i>via</i> layer-by-layer (LbL) synthesis. The LbL deposition enables the design of multishell systems, where each MOF shell can be modified to install different functions. Here, we used this approach to create controlled release capsules, in which the inner shell serves as a reservoir and the outer shell serves as a membrane after postsynthetic conversion of the MOF structure to a polymer network. These capsules enable the controlled release of loaded dye molecules, depending on the surrounding media