Influence of Plant-Based Biostimulant (BORTAN) on Qualitative and Aromatic Traits of Rocket Salad (Diplotaxis tenuifolia L.)

In this study, the influence of a new plant-based biostimulant (Bortan) on physiological and aromatic traits of rocket (Diplotaxis tenuifolia L. var. Pamela) was monitored by evaluating physico-chemical parameters (fresh and dry weight, leaf color and chlorophyll content) and biochemical traits (total phenolic compound (TP), total flavonoids (TF), ascorbic acid (AA) and antioxidant activity (AOX). Volatile profiles were also analyzed by headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry, allowing the detection of 32 volatiles belonging to 5 chemical classes. Compared to the control, Bortan application enhanced leaf pigment content, including chlorophyll a, b and carotenoids (+10%, +16% and +28%, respectively) and increased TP (+34%), TF (+26%), AA (+19%) amonts and AOX value (+16%). Principal component analysis revealed a significant discrimination between the two samples. Specifically, treated samples were mainly associated with ”green-leaf” volatiles, namely hexanal and 2-hexenal, 3-hexenal and 1-penten-3-one, while control rocket was directly correlated with several alcohols and to all isothiocyanates, associated with the sulfur-like odor of rocket. These findings can add further support, both for farmers and the agro-food industry, in choosing PBs as a new and sustainable practice in complementing enhanced yields with premium-quality produce. To confirm these preliminary data, further experiments are needed by enlarging the sample size, testing different concentrations of Bortan and/or using other food crops

Influence of Plant-Based Biostimulant (BORTAN) on Qualitative and Aromatic Traits of Rocket Salad (<i>Diplotaxis tenuifolia</i> L.)

In this study, the influence of a new plant-based biostimulant (Bortan) on physiological and aromatic traits of rocket (Diplotaxis tenuifolia L. var. Pamela) was monitored by evaluating physico-chemical parameters (fresh and dry weight, leaf color and chlorophyll content) and biochemical traits (total phenolic compound (TP), total flavonoids (TF), ascorbic acid (AA) and antioxidant activity (AOX). Volatile profiles were also analyzed by headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry, allowing the detection of 32 volatiles belonging to 5 chemical classes. Compared to the control, Bortan application enhanced leaf pigment content, including chlorophyll a, b and carotenoids (+10%, +16% and +28%, respectively) and increased TP (+34%), TF (+26%), AA (+19%) amonts and AOX value (+16%). Principal component analysis revealed a significant discrimination between the two samples. Specifically, treated samples were mainly associated with ”green-leaf” volatiles, namely hexanal and 2-hexenal, 3-hexenal and 1-penten-3-one, while control rocket was directly correlated with several alcohols and to all isothiocyanates, associated with the sulfur-like odor of rocket. These findings can add further support, both for farmers and the agro-food industry, in choosing PBs as a new and sustainable practice in complementing enhanced yields with premium-quality produce. To confirm these preliminary data, further experiments are needed by enlarging the sample size, testing different concentrations of Bortan and/or using other food crops

Discussion

Below is our discussion based on our results of our study

Materials

Listed below are the materials we used for this research

Introduction and Background

Below is an introduction into our project

Video of Procedure

Here is a brief video describing the procedure of this study. It is a silent walkthrough of everything one will do in their participation

Ethics Approval

This is our ethics approval from the IRB at our institute