Terpenoids from a multiple shoot culture of Telekia speciosa

Multiple shoots of Telekia speciosa were cultivated on MS medium containing 4.44 µM BAP and 0.54 µM NAA, solidified with agar. After eight weeks of culture the shoots were harvested and extracted with methanol. From the methanol extract one pseudoguaianolide – 2,3-dihydroaromaticin and three thymol derivatives: 8-hydroxy-9,10-diisobutyryloxythymol, 10-isobutyryloxy-8,9-epoxythymyl isobutyrate and 10-(2-methylbutyryloxy)-8,9-epoxythymyl isobutyrate were isolated as major secondary metabolites. Moreover, the shoots produced megastigmane and monoterpene glucosides, which were isolated for the first time from the species. The content of 2,3-dihydroaromaticin in the shoot culture was similar to that found in the intact plant, whereas yields of the three thymol derivatives were higher from multiple shoots than from the plants grown in the open field

Micropropagation of Urginea maritima (L.) Baker s. str.

A method of micropropagation of Urginea maritima (L.) Baker s.str. (Liliaceae) by adventitious shoot formation was developed. Bulb scales and leaf fragments were used as primary and secondary explants, respectively. The most favourable for shoot regeneration were media: MS supplemented with BAP or kinetin (bulb scales) and medium C containing NAA along with BAP (leaf explants). No difficulties in rooting and adapting of plants to greenhouse conditions were observed

Micropropagation of Scutellaria baicalensis Georgi

Plant propagation via nodal explants of Scutellaria baicalensis Georgi (Lamiaceae) was studied. Murashige and Skoog (MS) medium supplemented either with NAA and kinetin or NAA and BA in different concentrations was used for this purpose. The most favourable for axillary branching was MS medium supplemented with 0.5 µM NAA and 2.5 µM kinetin. The number of regenerated shoots per explant reached 3.8 ± 1.3. To increase the multiplication rate a two stage procedure of propagation was also elaborated. First, an induction of organogenic calli was achieved using thidiazuron (TDZ) as a sole growth regulator in the nutrient medium (concentration range: 0.1-0.5 µM). Next, regeneration of shoots on the medium containing 2.2 µM BA was performed. Over 20 shoots per explant could be obtained following the described procedure. The regenerated shoots rooted easily on a modified MS medium (1/2 macronutrients) and were successfully adapted to growth in pots