Correction to: A pair of gametologous genes provides further insights into avian comparative cytogenomics

Biologia https://doi.org/10.1007/s11756-023-01395-6 The original article has been updated to reflect added changes in the list of references. The original article has been corrected

A pair of gametologous genes provides further insights into avian comparative cytogenomics

Exploration of avian gametologous genes, i.e., homologous genes located on both the Z and W chromosomes, provides a crucial information about the underlying mechanism pertaining to the evolution of these chromosomes. The domestic chicken (Gallus gallus (Linnaeus 1758); GGA) traditionally serves as the primary reference subject of these comparative cytogenomic studies. Using bioinformatic, molecular (overgo BAC library scanning), and cytogenetic (BAC-based FISH) techniques, we have investigated in detail a pair of UBE2R2/UBE2R2L gametologs. By screening a gridded genomic jungle fowl BAC library, CHORI-261, with a short labeled UBE2R2L gene fragment called overgo probe, we detected seven specific clones. For three of them, CH261-019I23, CH261-105E16, and CH261-114G22, we identified their precise cytogenetic location on the Gallus gallus W chromosome (GGAW). They also co-localized with the UBAP2L2 gene on the, as was shown previously, along with the CH261-053P09 BAC clone also containing the GGAW-specific UBE2R2L DNA sequence. The fine mapping of the UBE2R2/UBE2R2L homologs in the chicken genome also shed the light on comparative cytogenetic aspects in birds. Our findings provided further evidence that bird genomes moderately changed only during evolution and are suitable for successful use of interspecies hybridization using both overgo-based BAC library screen and BAC-based FISH

[Cytogenetic localization of the genes on avian Z and W chromosomes with the use of large insert genomic clones] Цитогенетическая локализация генов на Z- и W- хромосомах птиц при помощи протяженных геномных клонов

Благовещенский И.Ю.; Сазанова А.Л.; Романов М.Н.; Фомичев К.А.; Стекольникова В.А.; Сазанов А.А

[Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones] Изучение псевдоаутосомных и граничащих с ними районов Z- и W-хромосом птиц при помощи протяженных геномных BAC-клонов

Благовещенский И.Ю., Сазанова А.Л., Стекольникова В.А., Фомичев К.А., Баркова О.Ю., Романов М.Н., Сазанов А.А. Для изучения псевдоаутосомных и граничащих с ними районов Z- и W-хромосом птиц были использованы семь BAC-клонов, обнаруженных в геномных библиотеках с использованием в качестве ДНК-зондов фрагментов различных гаметологов гена ATP5А1, расположенного вблизи проксимальной границы псевдоаутосомного района половых хромосом домашней курицы и японского перепела. Методом флуоресцентной гибридизации in situ с использованием W-специфических зондов определены локализация BAC-клонов TAM31-b100C09, TAM31-b99N01, TAM31-b27P16 и TAM31-b95L18 в коротком плече Z-хромосом домашней курицы и японского перепела (район Zp23–p22) и локализация BAC-клонов CHORI–261-CH46G16, CHORI-261-CH33F10 и CHORI-261-CH64F22 на W-хромосомах этих видов птиц и в коротком плече Z-хромосом (район Zp23–p22). Различия в локализации BAC-клонов на Z- и W-хромосомах, возможно, связаны с наличием дивергенции в нуклеотидных последовательностях разных половых хромосом, находящихся за пределами псевдоаутосомного района. To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region

Localization of seven HSA3q13-q23 NotI linking clones on the chicken microchromosomes 14 and 15 by double-color FISH

Double-color fluorescence in situ hybridization was performed on chicken chromosomes using seven unique clones from human chromosome 3-specific NotI linking libraries. Six of them (NL1-097, NL2-092, NL2-230, NLM-007, NLM-118, and NLM-196) hybridized to the same chicken microchromosome, while NL1-290 hybridized to another one. Two chicken microchromosome GGA15-specific BAC clones, JE024F14 containing the IGVPS gene and JE020G17 containing the ALDH1A1 gene, were cytogenetically mapped to the same microchromosome that contained the six NotI linking clones, allowing identification of this chromosome as GGA15. Two GGA14-specific clones, JE027C23 and JE014E08, containing the HBA gene cluster, were co-localized on the same microchromosome with NL1-290, marking this chromosome as GGA14. The results indicate that the human chromosome region HSA3q13-q23 is likely to be orthologous to GGA15 and GGA14. The synteny breakpoint between the human and chicken gene segments was detected on HSA3q13.3-q23 between NL1-290 and the six other NotI clones. Previously available comparative mapping data suggest another breakpoint between all the above NotI clones and four genes, TFRC, EIF4A2, SKIL and DHX36, located on HSA3q24-qter and GGA9. Microchromosomal location of seven NotI clones from the HSA3q21 T-band region can be considered as an evidence in support of our hypothesis regarding the functional analogy of mammalian T-bands and avian microchromosomes

Investigation of pseudoautosomal and bordering regions in avian Z and W chromosomes with the use of large insert genomic BAC clones

To study pseudoautosomal and bordering regions in the avian Z and W chromosomes, we used seven BAC clones from genomic libraries as DNA probes of fragments of different gametologs of the ATP5A1 gene located close to the proximal border of the pseudoautosomal region (PAR) of sex chromosomes of domestic chicken and Japanese quail. Localization of BAC clones TAM31-b100C09, TAM31-b99N01, TAM31-b27P16, and TAM31-b95L18 in the short arm of Z chromosomes of domestic chicken and Japanese quail (region Zp23-p22) and localization of the BAC clones CHORI-261-CH46G16, CHORI-261-CH33F10, and CHORI-261-CH64F22 on W chromosomes of these species and in the short arm of Z chromosomes (region Zp23-p22) were determined by fluorescence in situ hybridization with the use of W-specific probes. The difference in the localization of the BAC clones on the Z and W chromosomes is probably explained by divergence of the nucleotide sequences of different sex chromosomes located beyond the pseudoautosomal region

Chromosomal localization of CTSL: expanding of the region of evolutionary conservation between GGAZ and HSA9

Source/description: Cathepsin L (CTSL) is a lysosomal cysteine proteinase with a major role in intracellular protein catabolism. It also shows the most potent collagenolytic and elastinolytic activity in vitro of any of the cathepsins. We report here for the first time the cytogenetic assignment of the chicken CTSL gene..

Chromosomal localization of large insert clones of the chicken genome: expanding the comparative map

Fluorescent in situ hybridization (FISH) mapping provides a convenient method for aligning clone-based physical maps with the cytogenetic map of the chicken. This also provides framework marker locations for future cytogenetic mapping using dual label techniques. In the course of screening chicken BAC libraries with linkage map markers, several BACs were selected for FISH mapping. These clones derived from filter hybridization using conventional probes for chicken genes on chromosomes 1 (MGP, MGF, TYR), 4 (ALB, ANXA5, ENDRA) and Z (CTSL, NTRK2, TPM2, and UBAP2). Cytogenetic assignments were first done for seven genes (MGP, MGF, ANXA5, ENDRA, CTSL, TPM2, and UBAP2), and chromosome location was defined more precisely for three other genes (TYR, ALB, and NTRK2). These data provide new information for comparative mapping of conserved segments of orthologous genes between chicken and human chromosomes, including GGA1-HSA12, GGA1-HSA11, GGA4-HSA4 and GGAZ-HSA9. Acknowledgements: This work was supported by grants from the Russian Foundation for Basic Researches 03-04-48060-a, Russian Ministry of Education PD02-1.4-175 and INTAS 04-2163 and by the USDA/CSREES (Project numbers: 99-35205-8566 and 2001-52100-11225)