5 research outputs found
Results from PCR.
<p>A: PCR data show that pellets from whole blood samples (10 mL) spiked with higher concentrations of MSSA amplify sooner (pellet volume in PCR well = 8 μL). B: Contingency table for PCR analysis of processed positive (100 CFU/mL) and negative (0 CFU/mL) whole blood samples.</p
Graph showing the percent recoveries of viable microorganisms following our sample preparation protocol.
<p>Data are averages of several replicates (n>3).</p
Images of the pellets after lysing 10 mL of whole blood.
<p>A: Microscopic view of an unspiked blood sample after lysis process at 60x magnification; B: For reference, microscopic view of unlysed whole blood at 60x magnification; C: Front view of the pellets; D: Side view of the pellets; E: For reference, from left to right, 2.5, 5.0, 10, and 15 μL of whole blood.</p
List of microorganisms used in the current study and their cultivation conditions.
<p><sup><i>a</i></sup> Gram-positive bacterium;</p><p><sup><i>b</i></sup> Gram-negative bacterium;</p><p><sup><i>c</i></sup> Yeast</p><p>List of microorganisms used in the current study and their cultivation conditions.</p
Images of the custom-made microbial concentration devices.
<p>Left: a blown-up view of the individual parts of the device. Right: a CAD model of a fully assembled device.</p