Bar chart of the enriched biological functions of differentially expressed proteins in pFF+ versus pFF- samples.

<p>The assessment of significantly enriched biological functions for differentially expressed proteins was performed by IPA software. p-values and the number (#) of molecules mapped on the enriched categories are reported. </p

Mapping of a subset of differentially expressed proteins in pFF+ versus pFF- samples on the complement system canonical pathway.

<p>According to IPA categorization, up-regulated proteins are coloured in red. The assessment of significantly enriched processes networks for differentially expressed proteins was performed by evaluating the probability of a random intersection between the differentially expressed proteins with functional processes by applying the hypergeometric test. The pathway components identified by the algorithm or with no significant differences in their expression levels are reported in white and blue, respectively. Molecules are named according to IPA software.</p

Mapping of a subset of differentially expressed proteins in pFF+ versus pFF- samples on the acute phase response signaling canonical pathway.

<p>According to IPA categorization, up-regulated proteins are coloured in red. The assessment of significantly enriched processes networks for differentially expressed proteins was performed by evaluating the probability of a random intersection between the differentially expressed proteins with functional processes by applying the hypergeometric test. The pathway components identified by the algorithm or with no significant differences in their expression levels are reported in white and blue, respectively. Molecules are named according to IPA software.</p

Expression levels of chemokines in hFF samples.

<p>hFF samples were simultaneously screened for the concentration of the chemokines Eotaxin (A), IP-10 (B), MIP-1α (C), MIP-1β (D), MCP-1 (E) and RANTES (F) by using the Bio-Plex 27-plex human cytokine kit from BioRad according to the manufacturer’s protocol. Measurements were performed on individual hFF samples diluted (1:2) using the standard curves optimization and the calculation of analyte concentrations of the Bio-Plex Manager software. Data are reported as scatter plots and average concentrations. ** p-value≤ 0.05 FF+ <i>versus</i> FF- dataset.</p

Mapping of a subset of differentially expressed proteins in pFF+ versus pFF- samples on the coagulation system pathway.

<p>According to IPA categorization, up-regulated proteins are coloured in red. The assessment of significantly enriched processes networks for differentially expressed proteins was performed by evaluating the probability of a random intersection between the differentially expressed proteins with functional processes by applying the hypergeometric test. The pathway components identified by the algorithm or with no significant differences in their expression levels are reported in white and blue, respectively. Molecules are named according to IPA software.</p