Aoraki/Mt Cook and the Mackenzie Basin’s transition from wilderness to tourist place

The natural landscapes of New Zealand are a key attraction for domestic and international visitors for a variety of recreation and leisure purposes. This paper explores the interactions between heritage and recreational values for a region in New Zealand known for its sublime landscape (Bell and Lyall 2002). The paper discusses the transient movements and activities of visitors encountering this sociocultural landscape, often seeking to view the iconic landmark - Aoraki/Mt Cook – part of the Te Wahipounamu South West New Zealand World Heritage area – which is accessed via the Mackenzie Basin. Aoraki/Mt Cook National Park is a wilderness region that has significance not only for local inhabitants but also for travellers sightseeing and recreating in the area. Early inhabitants of the region – Māori from the Kai Tahu iwi (tribe) - visited the Mackenzie Basin‟s numerous waterways and lakes to gather mahika kai (traditional foods) and continue to have strong associations with the area. Since European settlement in the mid 19th century the Mackenzie landscape has been shaped by human activities, particularly farming, tourism and recreation

Why are You Here? Modeling Illicit Massage Business Location Characteristics with Machine Learning

Illicit massage businesses are a venue for sex and labor trafficking in the United States. Though many of their locations are made publicly available through online advertising, little is known about why they choose to locate where they do. In this work, we use inferential modeling to better understand the spatial distribution of illicit massage businesses within the U.S. Based on addresses web-scraped weekly from online advertisements over 6 months, we modeled illicit massage business prevalence at the census tract and county levels. We used publicly available data to characterize census tracts and counties, finding that the state in which they are located, distance to international airports, rent and income levels, racial composition, and religious presence all had significant relationships to illicit massage business presence. Illicit massage businesses operating with unethical labor practices and/or forced sexual services are not in line with just, peaceful, and inclusive societal goals, and the burdens of this industry disproportionately fall on women. While we emphasize that not all illicit massage businesses are settings of human trafficking, better understanding this industry is a key step toward better regulating it and protecting those harmed within it

Cybersecurity in UK Universities: mapping (or managing) threat intelligence sharing within the higher education sector

Higher education has recently been identified as a sector of concern by the UK National Cyber Security Centre (NCSC). In 2021, the NCSC reported that universities and higher education institutions (HEI) had been exponentially targeted by cyber-criminals. Existing challenges were amplified or highlighted over the course of the global pandemic when universities struggled to continue to function through hybrid and remote teaching provision that relied heavily on their digital estate and services. Despite the value of the sector and the vulnerabilities within it, higher education has received relatively little attention from the cybersecurity research community. Over 2 years, we carried out numerous interventions and engagements with the UK higher education sector. Through interviews with cybersecurity practitioners working in the sector as well as roundtables, and questionnaires, we conducted a qualitative and quantitative analysis of threat intelligence sharing, which we use as a proxy for measuring and analysing collaboration. In a unique approach to studying collaboration in cybersecurity, we utilized social network analysis. This paper presents the study and our findings about the state of cybersecurity in UK universities. It also presents some recommendations for future steps that we argue will be necessary to equip the higher education sector to continue to support UK national interests going forward. Key findings include the positive inclination of those working in university cybersecurity to collaborate as well as the factors that impede that collaboration. These include management and insurance constraints, concerns about individual and institutional reputational damage, a lack of trusted relationships, and the lack of effective mechanisms or channels for sectoral collaboration. In terms of the network itself, we found that it is highly fragmented with a very small number of the possible connections active, none of the organizations we might expect to facilitate collaboration in the network are playing a significant role, and some universities are currently acting as key information bridges. For these reasons, any changes that might be led by sectoral bodies such as Jisc, UCISA or government bodies such as NCSC, would need to go through these information brokers

Gene therapy for obstetric conditions

The first clinical trials of gene therapy in the 1990s offered the promise of a new paradigm for the treatment of genetic diseases. Over the decades that followed the challenges and setbacks which gene therapy faced often overshadowed any successes. Despite this, recent years have seen cause for renewed optimism. In 2012 Glybera™, an adeno-associated viral vector expressing lipoprotein lipase, became the first gene therapy product to receive marketing authorisation in Europe, with a licence to treat familial lipoprotein lipase deficiency. This followed the earlier licensing in China of two gene therapies: Gendicine™ for head and neck squamous cell carcinoma and Oncorine™ for late-stage nasopharyngeal cancer. By this stage over 1800 clinical trials had been, or were being, conducted worldwide, and the therapeutic targets had expanded far beyond purely genetic disorders. So far no trials of gene therapy have been carried out in pregnancy, but an increasing understanding of the molecular mechanisms underlying obstetric diseases means that it is likely to have a role to play in the future. This review will discuss how gene therapy works, its potential application in obstetric conditions and the risks and limitations associated with its use in this setting. It will also address the ethical and regulatory issues that will be faced by any potential clinical trial of gene therapy during pregnancy

The Histone Deacetylase Complex (HDC) 1 protein of Arabidopsis thaliana has the capacity to interact with multiple proteins including histone 3-binding proteins and histone 1 variants

Intrinsically disordered proteins can adopt multiple conformations thereby enabling interaction with a wide variety of partners. They often serve as hubs in protein interaction networks. We have previously shown that the Histone Deacetylase Complex (HDC) 1 protein from Arabidopsis thaliana interacts with histone deacetylases and quantitatively determines histone acetylation levels, transcriptional activity and several phenotypes, including ABA-sensitivity during germination, vegetative growth rate and flowering time. HDC1-type proteins are ubiquitous in plants but they contain no known structural or functional domains. Here we explored the protein interaction spectrum of HDC1. In addition to binding histone deacetylases, HDC1 directly interacted with core histone H3-binding proteins and co-repressor associated proteins, but not with H3 or the co-repressors themselves. Surprisingly, HDC1 was also able to interact with variants of the linker histone H1. Truncation of HDC1 to the ancestral core sequence narrowed the spectrum of interactions and of phenotypic outputs but maintained binding to a H3-binding protein and to H1. The results indicate a potential link between H1 and histone modifying complexes

Cryptic variation in RNA-directed DNA-methylation controls lateral root development when auxin signaling is perturbed

Maintaining the right balance between plasticity and robustness in biological systems is important to allow adaptation while maintaining essential functions. Developmental plasticity of plant root systems has been the subject of intensive research, but the mechanisms underpinning robustness remain unclear. Here, we show that potassium deficiency inhibits lateral root organogenesis by delaying early stages in the formation of lateral root primordia. However, the severity of the symptoms arising from this perturbation varies within a natural population of Arabidopsis and is associated with the genetic variation in CLSY1, a key component of the RNA-directed DNA-methylation machinery. Mechanistically, CLSY1 mediates the transcriptional repression of a negative regulator of root branching, IAA27, and promotes lateral root development when the auxin-dependent proteolysis pathway fails. Our study identifies DNA-methylation-mediated transcriptional repression as a backup system for post-translational protein degradation which ensures robust development and performance of plants in a challenging environment

Recent Advances in Cytogenetic Technology for Antenatal Genetic Testing

The examination of human chromosomes has been a part of the physician’s laboratory armamentarium since the correct diploid number of human chromosomes was established and a method was developed for the in vitro growth of peripheral blood leukocytes to yield metaphase chromosomes. The discovery that on ultraviolet microscopy (UV), metaphase chromosomes stained with fluorochrome dyes displayed a characteristic pattern of bright and dull bands unique for a given pair of homologous chromosomes, was a major technological breakthrough in human cytogenetics; for the first time, every chromosome in the karyotype could be unequivocally identified. Although the short storage life of fluorochrome-stained chromosomes and the costs of UV microscopy have limited the usability of fluorescence banding, the introduction of one discriminating procedure quickly led to the development of an array of similar banding techniques for conventional microscopy that yield comparable information. Some of these technical procedures depend on enzyme and/or heat denaturation of the chromosomes, resulting in the characteristic banding patterns seen by the trypsin-Giemsa method, the 5M urea method, and the acid-saline-Giemsa technique. A typical human karyotype prepared from metaphase chromosomes treated with trypsin, stained with Giemsa, and photographed with brightfield photomicrographic techniques is shown in Figure 1. Careful examination of this karyotype reveals that each chromosome in the homologous pair has an array of dark and light bands identical with those of its homolog and that each homologous pair, autosomes number 1 to number 22, has a characteristic, easily identifiable banding pattern

Metal Abundances of Intermediate-Redshift AGN: Evidence for a Population of Lower-Metallicty Seyfert 2 Galaxies at z = 0.3-0.4

We derive oxygen abundances for two samples of Seyfert 2 (Sy2) active galactic nuclei (AGN) selected from the KPNO International Spectroscopic Survey (KISS). The two samples from KISS include 17 intermediate-redshift (0.29 < z < 0.42) Sy2s detected via their [O III] lines, and 35 low-redshift (z < 0.1), Halpha-detected Sy2s. The primary goal of this work is to explore whether the metallicity distribution of these two samples changes with redshift. To determine the oxygen abundances of the KISS galaxies, we use Cloudy to create a large number of photoionization model grids by varying the temperature of the accretion disk, the ratio of X-ray to UV continuum light, the ionization parameter, the hydrogen density, and the metallicity of the narrow-line region clouds. We link the results of these models to the observed [O III]/H-beta and [N II]/H-alpha emission-line ratios of the KISS sample on the BPT diagram, interpolating across the model grids to derive metallicity. The two redshift samples overlap substantially in terms of derived metal abundances, but we find that some of the intermediate-redshift Sy2 galaxies possess lower abundances than their local universe counterparts. Our analysis provides evidence for modest levels of chemical evolution (0.18 +/- 0.06 dex) over 3-4 Gyrs of look-back time. We compare our results to other AGN abundance derivation methods from the literature.Comment: 17 pages, 12 figures; accepted for publication in the Astrophysical Journa

Is video modeling enough to teach parent-child interactions? Toward a systematic evaluation of the key components of video modeling.

Parent-child interactions help set the foundation for a child's development. It is therefore important to investigate the relative efficiency and efficacy of procedures used to train them. One procedure that researchers continue to explore is video modeling. The current study evaluated the effect of a video model that displayed favorable parent-child interactions and a modified model with embedded instructions to determine if the introduction of either of these models would alter parent-child interactions. Both models were presented alone without supplemental guidance. Three families were involved in the study. The results showed no systematic change across families or conditions as a result of video viewing and are discussed within context of the needs of the parent, adequate stimulus control, community to support behavior change, measurement sensitivity, and influence of methodology. This study provided a great baseline for future studies to explore the necessary components to create an effective video model

Development and evaluation of an interactive multimedia clinical skills teaching program designed for the pediatric clerkship.

Background and Purpose: The physical examination section of a multimedia program developed to teach infant history and physical examination skills was evaluated. Methods: 71 students participated: one group viewed only the physical examination section (PX), one the history section (HX), one none of the program (CX). Physical examination skills were assessed by direct observation of medical students performing an abdominal exam and scored using a checklist at baseline, immediately after intervention and at the end of the pediatric clerkship. Results were analyzed using ANOVA with repeated measures. Results: Baseline scores were: PX 2.5; HX 2.8. The PX group scored significantly higher immediately post intervention at 6.8 compared to the HX group 3.1. At the end of the clerkship significant differences between the groups remained. Final group mean scores were: PX 5.5, HX 4.4 and CX 2.7. Conclusion: The program improved examination skills with attenuation over 6 weeks