3 research outputs found

    Morphological Differentiation, Mitochondrial and Nuclear DNA Variability Between Geographically Distant Populations of Daphnia galeata and Daphnia cucullata (Anomopoda, Daphniidae)

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    НСсмотря Π½Π° Ρ‚ΠΎ, Ρ‡Ρ‚ΠΎ прСдставитСли Ρ€. Daphnia (Anomopoda, Daphniidae) ΡΠ²Π»ΡΡŽΡ‚ΡΡ ΠΎΠ΄Π½ΠΈΠΌΠΈ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ распространСнных Π²ΠΎΠ΄Π½Ρ‹Ρ… бСспозвоночных ΠΈ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΡŽΡ‚ΡΡ Π² качСствС ΠΌΠΎΠ΄Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π² таксономичСских, экологичСских ΠΈ ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠΎΠ½Π½Ρ‹Ρ… исслСдованиях, ΠΈΡ… систСматика остаСтся вСсьма Π·Π°ΠΏΡƒΡ‚Π°Π½Π½ΠΎΠΉ. НастоящСС исслСдованиС посвящСно ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΡŽ морфологичСской Π΄ΠΈΡ„Ρ„Π΅Ρ€Π΅Π½Ρ†ΠΈΠ°Ρ†ΠΈΠΈ ΠΈ гСнСтичСской измСнчивости гСографичСски ΡƒΠ΄Π°Π»Π΅Π½Π½Ρ‹Ρ… популяций сСстринских Π²ΠΈΠ΄ΠΎΠ² Daphnia galeata Sars, 1864 ΠΈ Daphnia cucullata Sars, 1862 (Anomopoda, Daphniidae) ΠΈΠ· прСсноводной части Балтийского моря - ΠšΡƒΡ€ΡˆΡΠΊΠΎΠ³ΠΎ Π·Π°Π»ΠΈΠ²Π° (Россия, ΠšΠ°Π»ΠΈΠ½ΠΈΠ½Π³Ρ€Π°Π΄ΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡ‚ΡŒ) ΠΈ Новосибирского Π²ΠΎΠ΄ΠΎΡ…Ρ€Π°Π½ΠΈΠ»ΠΈΡ‰Π° (Россия, Новосибирская ΠΎΠ±Π»Π°ΡΡ‚ΡŒ). ΠœΠΎΡ€Ρ„ΠΎΠ»ΠΎΠ³ΠΈΡ‡Π΅ΡΠΊΠ°Ρ дивСргСнция ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ ΠΈ ΠΈΡ… популяциями ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π»Π°ΡΡŒ ΠΏΠΎ диагностичСским ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠ°ΠΌ ΠΈ Π½Π° основании Π°Π½Π°Π»ΠΈΠ·Π° измСнчивости Ρ„ΠΎΡ€ΠΌΡ‹ Ρ‚Π΅Π»Π° ΠΏΠΎ Π½Π°Π±ΠΎΡ€Ρƒ морфомСтричСских ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΎΠ². Π‘Π°ΠΌΡ‹ΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½Ρ‡ΠΈΠ²Ρ‹ΠΌΠΈ Π±Ρ‹Π»ΠΈ ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΈ, Ρ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΡƒΡŽΡ‰ΠΈΠ΅ Ρ„ΠΎΡ€ΠΌΡƒ Π³ΠΎΠ»ΠΎΠ²Ρ‹, шлСма ΠΈ хвостовой ΠΈΠ³Π»Ρ‹. РСконструкция филогСнСтичСских ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ Π²Ρ‹ΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° основС измСнчивости 16S ΠΈ 12S Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡ‚ΠΎΡ…ΠΎΠ½Π΄Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ Π”ΠΠš ΠΈ Ρ„Ρ€Π°Π³ΠΌΠ΅Π½Ρ‚Π° ITS2 ядСрной Π”ΠΠš. ДивСргСнция ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ D. galeata ΠΈ D. cucullata Π½Π° основС Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡ‚ΠΎΡ…ΠΎΠ½Π΄Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ Π”ΠΠš Π±Ρ‹Π»Π° Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ ΠΈ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΠ΅Ρ‚ ΠΎΠ± ΠΈΡ… монофилСтичСском происхоТдСнии, Ρ‚ΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Π²Π½ΡƒΡ‚Ρ€ΠΈΠ²ΠΈΠ΄ΠΎΠ²Ρ‹Π΅ гСнСтичСскиС дистанции ΠΎΡ†Π΅Π½ΠΈΠ²Π°ΡŽΡ‚ΡΡ ΠΊΠ°ΠΊ Π½Π΅Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅.Although members of genus Daphnia (Anomopoda, Daphniidae) are the most common water invertebrates and are considered as model organisms for many taxonomic, ecological and evolutionary studies their systematics remains unresolved. Here, morphological differentiation and genetic polymorphism between the geographically distant populations of the sister species Daphnia galeata Sars, 1864 and Daphnia cucullata Sars, 1862 in the Curonian Lagoon, a large shallow freshwater lagoon of the Baltic Sea (Russia, Kaliningrad Oblast) and Novosibirsk Reservoir (Russia, Novosibirsk Oblast) are presented. The divergence between species and their populations was analyzed based on traditional morphological traits and a large set of morphometric traits describing the body shape. The traits describing the shape of head and helmet, and spine were the most variable morphological characters. Phylogenetic relationships between species and populations were constructed based on variation in mitochondrial 16S and 12S rRNA genes and nuclear ITS2 rDNA sequences. The mitochondrial DNA divergence between D. galeata and D. cucullata species was significant and reflected their monophyletic origin, whereas intraspecific genetic distances are estimated as insignificant

    Role of Nerve Growth Factor in Assessing the Severity of Clinical Manifestations and Outcomes of Perinatal CNS Lesions in Infants

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    The purpose of our research was to analyze the association between the serum beta-NGF level and the severity of neurological deficit (ND) in children in the first year of life. Our results suggest a possible functional link between the low level of NGF and the development of severe ND. The obtained results allow us to consider the serum beta-NGF level as a useful marker of the ND severity in young children

    Morphological Differentiation, Mitochondrial and Nuclear DNA Variability Between Geographically Distant Populations of Daphnia galeata and Daphnia cucullata (Anomopoda, Daphniidae)

    No full text
    НСсмотря Π½Π° Ρ‚ΠΎ, Ρ‡Ρ‚ΠΎ прСдставитСли Ρ€. Daphnia (Anomopoda, Daphniidae) ΡΠ²Π»ΡΡŽΡ‚ΡΡ ΠΎΠ΄Π½ΠΈΠΌΠΈ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ распространСнных Π²ΠΎΠ΄Π½Ρ‹Ρ… бСспозвоночных ΠΈ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΡŽΡ‚ΡΡ Π² качСствС ΠΌΠΎΠ΄Π΅Π»ΡŒΠ½Ρ‹Ρ… ΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π² таксономичСских, экологичСских ΠΈ ΡΠ²ΠΎΠ»ΡŽΡ†ΠΈΠΎΠ½Π½Ρ‹Ρ… исслСдованиях, ΠΈΡ… систСматика остаСтся вСсьма Π·Π°ΠΏΡƒΡ‚Π°Π½Π½ΠΎΠΉ. НастоящСС исслСдованиС посвящСно ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΡŽ морфологичСской Π΄ΠΈΡ„Ρ„Π΅Ρ€Π΅Π½Ρ†ΠΈΠ°Ρ†ΠΈΠΈ ΠΈ гСнСтичСской измСнчивости гСографичСски ΡƒΠ΄Π°Π»Π΅Π½Π½Ρ‹Ρ… популяций сСстринских Π²ΠΈΠ΄ΠΎΠ² Daphnia galeata Sars, 1864 ΠΈ Daphnia cucullata Sars, 1862 (Anomopoda, Daphniidae) ΠΈΠ· прСсноводной части Балтийского моря - ΠšΡƒΡ€ΡˆΡΠΊΠΎΠ³ΠΎ Π·Π°Π»ΠΈΠ²Π° (Россия, ΠšΠ°Π»ΠΈΠ½ΠΈΠ½Π³Ρ€Π°Π΄ΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡ‚ΡŒ) ΠΈ Новосибирского Π²ΠΎΠ΄ΠΎΡ…Ρ€Π°Π½ΠΈΠ»ΠΈΡ‰Π° (Россия, Новосибирская ΠΎΠ±Π»Π°ΡΡ‚ΡŒ). ΠœΠΎΡ€Ρ„ΠΎΠ»ΠΎΠ³ΠΈΡ‡Π΅ΡΠΊΠ°Ρ дивСргСнция ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ ΠΈ ΠΈΡ… популяциями ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π»Π°ΡΡŒ ΠΏΠΎ диагностичСским ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠ°ΠΌ ΠΈ Π½Π° основании Π°Π½Π°Π»ΠΈΠ·Π° измСнчивости Ρ„ΠΎΡ€ΠΌΡ‹ Ρ‚Π΅Π»Π° ΠΏΠΎ Π½Π°Π±ΠΎΡ€Ρƒ морфомСтричСских ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΎΠ². Π‘Π°ΠΌΡ‹ΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½Ρ‡ΠΈΠ²Ρ‹ΠΌΠΈ Π±Ρ‹Π»ΠΈ ΠΏΡ€ΠΈΠ·Π½Π°ΠΊΠΈ, Ρ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΡƒΡŽΡ‰ΠΈΠ΅ Ρ„ΠΎΡ€ΠΌΡƒ Π³ΠΎΠ»ΠΎΠ²Ρ‹, шлСма ΠΈ хвостовой ΠΈΠ³Π»Ρ‹. РСконструкция филогСнСтичСских ΠΎΡ‚Π½ΠΎΡˆΠ΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ Π²Ρ‹ΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° основС измСнчивости 16S ΠΈ 12S Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡ‚ΠΎΡ…ΠΎΠ½Π΄Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ Π”ΠΠš ΠΈ Ρ„Ρ€Π°Π³ΠΌΠ΅Π½Ρ‚Π° ITS2 ядСрной Π”ΠΠš. ДивСргСнция ΠΌΠ΅ΠΆΠ΄Ρƒ Π²ΠΈΠ΄Π°ΠΌΠΈ D. galeata ΠΈ D. cucullata Π½Π° основС Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡ‚ΠΎΡ…ΠΎΠ½Π΄Ρ€ΠΈΠ°Π»ΡŒΠ½ΠΎΠΉ Π”ΠΠš Π±Ρ‹Π»Π° Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠΉ ΠΈ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΠ΅Ρ‚ ΠΎΠ± ΠΈΡ… монофилСтичСском происхоТдСнии, Ρ‚ΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ Π²Π½ΡƒΡ‚Ρ€ΠΈΠ²ΠΈΠ΄ΠΎΠ²Ρ‹Π΅ гСнСтичСскиС дистанции ΠΎΡ†Π΅Π½ΠΈΠ²Π°ΡŽΡ‚ΡΡ ΠΊΠ°ΠΊ Π½Π΅Π·Π½Π°Ρ‡ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Π΅.Although members of genus Daphnia (Anomopoda, Daphniidae) are the most common water invertebrates and are considered as model organisms for many taxonomic, ecological and evolutionary studies their systematics remains unresolved. Here, morphological differentiation and genetic polymorphism between the geographically distant populations of the sister species Daphnia galeata Sars, 1864 and Daphnia cucullata Sars, 1862 in the Curonian Lagoon, a large shallow freshwater lagoon of the Baltic Sea (Russia, Kaliningrad Oblast) and Novosibirsk Reservoir (Russia, Novosibirsk Oblast) are presented. The divergence between species and their populations was analyzed based on traditional morphological traits and a large set of morphometric traits describing the body shape. The traits describing the shape of head and helmet, and spine were the most variable morphological characters. Phylogenetic relationships between species and populations were constructed based on variation in mitochondrial 16S and 12S rRNA genes and nuclear ITS2 rDNA sequences. The mitochondrial DNA divergence between D. galeata and D. cucullata species was significant and reflected their monophyletic origin, whereas intraspecific genetic distances are estimated as insignificant
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