3 research outputs found
Morphological Differentiation, Mitochondrial and Nuclear DNA Variability Between Geographically Distant Populations of Daphnia galeata and Daphnia cucullata (Anomopoda, Daphniidae)
ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΠΈ Ρ. Daphnia (Anomopoda, Daphniidae) ΡΠ²Π»ΡΡΡΡΡ
ΠΎΠ΄Π½ΠΈΠΌΠΈ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
Π²ΠΎΠ΄Π½ΡΡ
Π±Π΅ΡΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ
ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅
ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π² ΡΠ°ΠΊΡΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
, ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
, ΠΈΡ
ΡΠΈΡΡΠ΅ΠΌΠ°ΡΠΈΠΊΠ° ΠΎΡΡΠ°Π΅ΡΡΡ Π²Π΅ΡΡΠΌΠ° Π·Π°ΠΏΡΡΠ°Π½Π½ΠΎΠΉ. ΠΠ°ΡΡΠΎΡΡΠ΅Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ
ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°ΡΠΈΠΈ ΠΈ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ Π³Π΅ΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ ΡΠ΅ΡΡΡΠΈΠ½ΡΠΊΠΈΡ
Π²ΠΈΠ΄ΠΎΠ² Daphnia galeata Sars, 1864 ΠΈ Daphnia cucullata Sars, 1862
(Anomopoda, Daphniidae) ΠΈΠ· ΠΏΡΠ΅ΡΠ½ΠΎΠ²ΠΎΠ΄Π½ΠΎΠΉ ΡΠ°ΡΡΠΈ ΠΠ°Π»ΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΌΠΎΡΡ - ΠΡΡΡΡΠΊΠΎΠ³ΠΎ Π·Π°Π»ΠΈΠ²Π° (Π ΠΎΡΡΠΈΡ,
ΠΠ°Π»ΠΈΠ½ΠΈΠ½Π³ΡΠ°Π΄ΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡΡ) ΠΈ ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠΎΠ³ΠΎ Π²ΠΎΠ΄ΠΎΡ
ΡΠ°Π½ΠΈΠ»ΠΈΡΠ° (Π ΠΎΡΡΠΈΡ, ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡΡ).
ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Π΄ΠΈΠ²Π΅ΡΠ³Π΅Π½ΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ ΠΈ ΠΈΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡΠΌΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»Π°ΡΡ ΠΏΠΎ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌ ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π° ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ ΡΠΎΡΠΌΡ ΡΠ΅Π»Π° ΠΏΠΎ Π½Π°Π±ΠΎΡΡ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ². Π‘Π°ΠΌΡΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΡΠΌΠΈ Π±ΡΠ»ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠΈΠ΅ ΡΠΎΡΠΌΡ Π³ΠΎΠ»ΠΎΠ²Ρ, ΡΠ»Π΅ΠΌΠ° ΠΈ
Ρ
Π²ΠΎΡΡΠΎΠ²ΠΎΠΉ ΠΈΠ³Π»Ρ. Π Π΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ ΡΠΈΠ»ΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π°
ΠΎΡΠ½ΠΎΠ²Π΅ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ 16S ΠΈ 12S Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ ΠΈ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° ITS2 ΡΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΠΠ.
ΠΠΈΠ²Π΅ΡΠ³Π΅Π½ΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ D. galeata ΠΈ D. cucullata Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ
Π±ΡΠ»Π° Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΈ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎΠ± ΠΈΡ
ΠΌΠΎΠ½ΠΎΡΠΈΠ»Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠΈ, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ
Π²Π½ΡΡΡΠΈΠ²ΠΈΠ΄ΠΎΠ²ΡΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄ΠΈΡΡΠ°Π½ΡΠΈΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅.Although members of genus Daphnia (Anomopoda, Daphniidae) are the most common water
invertebrates and are considered as model organisms for many taxonomic, ecological and
evolutionary studies their systematics remains unresolved. Here, morphological differentiation
and genetic polymorphism between the geographically distant populations of the sister species
Daphnia galeata Sars, 1864 and Daphnia cucullata Sars, 1862 in the Curonian Lagoon, a large
shallow freshwater lagoon of the Baltic Sea (Russia, Kaliningrad Oblast) and Novosibirsk Reservoir
(Russia, Novosibirsk Oblast) are presented. The divergence between species and their populations
was analyzed based on traditional morphological traits and a large set of morphometric traits
describing the body shape. The traits describing the shape of head and helmet, and spine were the
most variable morphological characters. Phylogenetic relationships between species and populations
were constructed based on variation in mitochondrial 16S and 12S rRNA genes and nuclear ITS2
rDNA sequences. The mitochondrial DNA divergence between D. galeata and D. cucullata species
was significant and reflected their monophyletic origin, whereas intraspecific genetic distances are
estimated as insignificant
Role of Nerve Growth Factor in Assessing the Severity of Clinical Manifestations and Outcomes of Perinatal CNS Lesions in Infants
The purpose of our research was to analyze the association between the serum beta-NGF level and the severity of neurological deficit (ND) in children in the first year of life. Our results suggest a possible functional link between the low level of NGF and the development of severe ND. The obtained results allow us to consider the serum beta-NGF level as a useful marker of the ND severity in young children
Morphological Differentiation, Mitochondrial and Nuclear DNA Variability Between Geographically Distant Populations of Daphnia galeata and Daphnia cucullata (Anomopoda, Daphniidae)
ΠΠ΅ΡΠΌΠΎΡΡΡ Π½Π° ΡΠΎ, ΡΡΠΎ ΠΏΡΠ΅Π΄ΡΡΠ°Π²ΠΈΡΠ΅Π»ΠΈ Ρ. Daphnia (Anomopoda, Daphniidae) ΡΠ²Π»ΡΡΡΡΡ
ΠΎΠ΄Π½ΠΈΠΌΠΈ ΠΈΠ· Π½Π°ΠΈΠ±ΠΎΠ»Π΅Π΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΡΡ
Π²ΠΎΠ΄Π½ΡΡ
Π±Π΅ΡΠΏΠΎΠ·Π²ΠΎΠ½ΠΎΡΠ½ΡΡ
ΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΡΡ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅
ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΡΡ
ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠΎΠ² Π² ΡΠ°ΠΊΡΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
, ΡΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠ²ΠΎΠ»ΡΡΠΈΠΎΠ½Π½ΡΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡΡ
, ΠΈΡ
ΡΠΈΡΡΠ΅ΠΌΠ°ΡΠΈΠΊΠ° ΠΎΡΡΠ°Π΅ΡΡΡ Π²Π΅ΡΡΠΌΠ° Π·Π°ΠΏΡΡΠ°Π½Π½ΠΎΠΉ. ΠΠ°ΡΡΠΎΡΡΠ΅Π΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠΎΡΠ²ΡΡΠ΅Π½ΠΎ ΠΈΠ·ΡΡΠ΅Π½ΠΈΡ
ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΠ°ΡΠΈΠΈ ΠΈ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ Π³Π΅ΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈ ΡΠ΄Π°Π»Π΅Π½Π½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ ΡΠ΅ΡΡΡΠΈΠ½ΡΠΊΠΈΡ
Π²ΠΈΠ΄ΠΎΠ² Daphnia galeata Sars, 1864 ΠΈ Daphnia cucullata Sars, 1862
(Anomopoda, Daphniidae) ΠΈΠ· ΠΏΡΠ΅ΡΠ½ΠΎΠ²ΠΎΠ΄Π½ΠΎΠΉ ΡΠ°ΡΡΠΈ ΠΠ°Π»ΡΠΈΠΉΡΠΊΠΎΠ³ΠΎ ΠΌΠΎΡΡ - ΠΡΡΡΡΠΊΠΎΠ³ΠΎ Π·Π°Π»ΠΈΠ²Π° (Π ΠΎΡΡΠΈΡ,
ΠΠ°Π»ΠΈΠ½ΠΈΠ½Π³ΡΠ°Π΄ΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡΡ) ΠΈ ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠΎΠ³ΠΎ Π²ΠΎΠ΄ΠΎΡ
ΡΠ°Π½ΠΈΠ»ΠΈΡΠ° (Π ΠΎΡΡΠΈΡ, ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ°Ρ ΠΎΠ±Π»Π°ΡΡΡ).
ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠ°Ρ Π΄ΠΈΠ²Π΅ΡΠ³Π΅Π½ΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ ΠΈ ΠΈΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡΠΌΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»Π°ΡΡ ΠΏΠΎ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌ ΠΈ Π½Π° ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π½Π°Π»ΠΈΠ·Π° ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ ΡΠΎΡΠΌΡ ΡΠ΅Π»Π° ΠΏΠΎ Π½Π°Π±ΠΎΡΡ ΠΌΠΎΡΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ². Π‘Π°ΠΌΡΠΌΠΈ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΡΠΌΠΈ Π±ΡΠ»ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠΈ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΡΡΠΈΠ΅ ΡΠΎΡΠΌΡ Π³ΠΎΠ»ΠΎΠ²Ρ, ΡΠ»Π΅ΠΌΠ° ΠΈ
Ρ
Π²ΠΎΡΡΠΎΠ²ΠΎΠΉ ΠΈΠ³Π»Ρ. Π Π΅ΠΊΠΎΠ½ΡΡΡΡΠΊΡΠΈΡ ΡΠΈΠ»ΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π°
ΠΎΡΠ½ΠΎΠ²Π΅ ΠΈΠ·ΠΌΠ΅Π½ΡΠΈΠ²ΠΎΡΡΠΈ 16S ΠΈ 12S Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ ΠΈ ΡΡΠ°Π³ΠΌΠ΅Π½ΡΠ° ITS2 ΡΠ΄Π΅ΡΠ½ΠΎΠΉ ΠΠΠ.
ΠΠΈΠ²Π΅ΡΠ³Π΅Π½ΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π²ΠΈΠ΄Π°ΠΌΠΈ D. galeata ΠΈ D. cucullata Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π³Π΅Π½ΠΎΠ² ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΠΠ
Π±ΡΠ»Π° Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΈ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΠ΅Ρ ΠΎΠ± ΠΈΡ
ΠΌΠΎΠ½ΠΎΡΠΈΠ»Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΠΈ, ΡΠΎΠ³Π΄Π° ΠΊΠ°ΠΊ
Π²Π½ΡΡΡΠΈΠ²ΠΈΠ΄ΠΎΠ²ΡΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π΄ΠΈΡΡΠ°Π½ΡΠΈΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°ΡΡΡΡ ΠΊΠ°ΠΊ Π½Π΅Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅.Although members of genus Daphnia (Anomopoda, Daphniidae) are the most common water
invertebrates and are considered as model organisms for many taxonomic, ecological and
evolutionary studies their systematics remains unresolved. Here, morphological differentiation
and genetic polymorphism between the geographically distant populations of the sister species
Daphnia galeata Sars, 1864 and Daphnia cucullata Sars, 1862 in the Curonian Lagoon, a large
shallow freshwater lagoon of the Baltic Sea (Russia, Kaliningrad Oblast) and Novosibirsk Reservoir
(Russia, Novosibirsk Oblast) are presented. The divergence between species and their populations
was analyzed based on traditional morphological traits and a large set of morphometric traits
describing the body shape. The traits describing the shape of head and helmet, and spine were the
most variable morphological characters. Phylogenetic relationships between species and populations
were constructed based on variation in mitochondrial 16S and 12S rRNA genes and nuclear ITS2
rDNA sequences. The mitochondrial DNA divergence between D. galeata and D. cucullata species
was significant and reflected their monophyletic origin, whereas intraspecific genetic distances are
estimated as insignificant