Limit of detection ^a of the fungal Luminex assay.

<p>Limit of detection <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173390#t006fn001" target="_blank">^a</a> of the fungal Luminex assay.</p

Multiplex xMAP^® analysis to test the bead- probe specificity.

<p>Multiplex xMAP^® analysis to test the bead- probe specificity.</p

Simplex xMAP^® analysis.

<p>Simplex xMAP^® analysis.</p

Proof of concept with environmental samples: Culture, microscopic determination and quantification.

<p>Proof of concept with environmental samples: Culture, microscopic determination and quantification.</p

Fungal species and probes used in this study.

<p>Fungal species and probes used in this study.</p

DNA Mixes analysis.

<p>DNA Mixes analysis.</p

Theoretical genomic copy number estimation of the LOD.

<p>Theoretical genomic copy number estimation of the LOD.</p

Proof of concept with environmental samples: Luminex xMAP^® analysis.

<p>Proof of concept with environmental samples: Luminex xMAP^® analysis.</p

Additional file 1: of Relationships between aeroallergen levels and hospital admissions for asthma in the Brussels-Capital Region: a daily time series analysis

Table S1. Spearman’s correlation coefficients among aeroallergens, Brussels-Capital Region, 2008–2013. Table S2. Confounding by air pollutants, influenza epidemics and general respiratory infections - Cumulative (lag 0–6 days) percentage change (95% confidence interval) in asthma hospitalizations associated with an interquartile range increase in pollen or fungal spore concentrations, Brussels-Capital Region, 2008–2013. Table S3. Sensitivity analyses - Cumulative (lag 0–6 days) percentage change (95% confidence interval) in asthma hospitalizations associated with an interquartile range increase in pollen or fungal spore concentrations, Brussels-Capital Region, 2008–2013. Figure S1. Cumulative (lag 0–6) exposure-response (ER) functions for the association between asthma admissions and some aeroallergen concentrations - Functions modelled using a natural cubic spline–natural cubic spline DLNM with 3 degrees of freedom (df) for the ER function and 4 df for the lag structure. Relative risks (RR) are relative to the reference value of 0 grains/m3. The vertical dotted lines represent the 75th, 95th and 99th percentiles of the pollen concentrations). (DOCX 37 kb