Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids-4

D DAPI (blue, nuclei) as described in the Methods section. (A'-D') TLRR signal corresponding to each of the merged images shown in A-D. Increasingly elongated spermatids are shown from panels A, A' through D, D'. TLRR is associated with the manchette in mid stage spermatids (arrowheads, panels C and C') and this staining narrows with the elongation of the manchette in approximately step 15 spermatids (arrowheads, panels D and D'). (E, E') Negative control for these experiments where TLRR specific primary antibody was omitted. Bar represents 10 μm.<p><b>Copyright information:</b></p><p>Taken from "Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids"</p><p>http://www.biomedcentral.com/1471-2121/9/9</p><p>BMC Cell Biology 2008;9():9-9.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2270827.</p><p></p

Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids-0

Leucine-rich repeats (LRRs) are indicated with solid underlining and the peptide used for preparation of antibody indicated with dashed underlining. The putative PP1 binding site is boxed. (B) The TLRR repeats are aligned with the LRR consensus sequence for the sds22 subfamily of LRR proteins. (C) An alignment between TLRR and Ppp1r7 is shown with the LRRs indicated with overlines (TLRR) and underlines (Ppp1r7). Multiple sequence alignment was done using ClustalW 1.82 and the results displayed using BOXSHADE 3.31. LRRs in TLRR and Ppp1r7 were identified using the Pfam protein domain database [35].<p><b>Copyright information:</b></p><p>Taken from "Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids"</p><p>http://www.biomedcentral.com/1471-2121/9/9</p><p>BMC Cell Biology 2008;9():9-9.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2270827.</p><p></p

Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids-2

E LRR domains are indicated with black boxes and below are shown the regions tested for interaction with the KIFC1 bait plasmid and the results of interaction tests. (B) Plate assay of interaction of TLRR deletion constructs with KIFC1bait. The plate on the left selects only for yeast harboring both bait and prey plasmids; all cotransformants are able to grow. The plate on the right also lacks adenine and histidine and is therefore selective for transactivation of the reporter genes. Transactivation of the MEL1 reporter gene is detected by incorporation of X-α-gal into the media resulting in blue colonies. Positive control for protein interaction (+C) is yeast cotransformed with pGADT7-T and pGBKT7-53 while cells containing pGADT7 and pGBKT7-Lam (-C) is negative control. (C) Liquid assay of interaction of TLRR deletion constructs with KIFC1bait. The β-galactosidase activity of cultures of TLRR/KIFC1 bait cotransformants was determined as described in Methods. Each assay was repeated at least three times. Standard error of the mean for each transformant is indicated by bracketed lines in panel C.<p><b>Copyright information:</b></p><p>Taken from "Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids"</p><p>http://www.biomedcentral.com/1471-2121/9/9</p><p>BMC Cell Biology 2008;9():9-9.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2270827.</p><p></p

Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids-6

Leucine-rich repeats (LRRs) are indicated with solid underlining and the peptide used for preparation of antibody indicated with dashed underlining. The putative PP1 binding site is boxed. (B) The TLRR repeats are aligned with the LRR consensus sequence for the sds22 subfamily of LRR proteins. (C) An alignment between TLRR and Ppp1r7 is shown with the LRRs indicated with overlines (TLRR) and underlines (Ppp1r7). Multiple sequence alignment was done using ClustalW 1.82 and the results displayed using BOXSHADE 3.31. LRRs in TLRR and Ppp1r7 were identified using the Pfam protein domain database [35].<p><b>Copyright information:</b></p><p>Taken from "Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids"</p><p>http://www.biomedcentral.com/1471-2121/9/9</p><p>BMC Cell Biology 2008;9():9-9.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2270827.</p><p></p