Clearigate ® Treatments for Control of Giant Salvinia

Results of recent field trials using the chelated copper formulation Clearigate® 4 showed that applying a 20% solution by volume was effective for controlling populations of giant salvinia in irrigation canals. 5 Lower rates may be efficacious, thereby reducing chemical use and cost; however, little is known about the dose-response effects of Clearigate® against giant salvinia. The objective of this study was to determine the effective rate range of chelated copper applied as Clearigate® for control of giant salvinia

Five-minute Apgar score and educational outcomes: retrospective cohort study of 751 369 children

Background: The Apgar score is used worldwide for assessing the clinical condition and short-term prognosis of newborn infants. Evidence for a relationship with long-term educational outcomes is conflicting. We investigated whether Apgar score at 5 min after birth was associated with additional support needs (ASN) and educational attainment. Methods: Data on pregnancy, delivery and later educational outcomes for children attending Scottish schools between 2006 and 2011 were collated by linking individual-level data from national educational and maternity databases. The relationship between Apgar score and overall ASN, type-specific ASN and educational attainment was assessed using binary, multinomial and generalised ordinal logistic regression models, respectively. Missing covariate data were imputed. Results: Of the 751 369 children eligible, 9741 (1.3%) had a low or intermediate Apgar score and 49 962 (6.6%) had ASN. Low Apgar score was independently associated with overall ASN status (adjusted OR for Apgar ≤3, OR 1.52 95% CI 1.35 to 1.70), as well as ASN due to cognitive (OR 1.26, 95% CI 1.09 to 1.47), sensory (OR 2.49 95% CI 1.66 to 3.73) and motor (OR 3.57, 95% CI 2.86 to 4.47) impairments. There was a dose-response relationship between Apgar score and overall ASN status: of those scoring 0–3, 10.1% had ASN, compared with 9.1% of those scoring 4–7 and 6.6% of those scoring 7–10. A low Apgar score was associated with lower educational attainment, but this was not robust to adjustment for confounders. Conclusions: Apgar scores are associated with long-term as well as short-term prognoses, and with educational as well as clinical outcomes at the population level

Human monoclonal antibodies to Plasmodium falciparum circumsporozoite protein for transient passive protection of malaria travelers to endemic areas

Plasmodium falciparum, is a protozoa that causes over 214 million cases of Malaria worldwide and the World Health Organization reported an estimated 438,000 deaths attributed to malaria in 2015. Current prevention strategies have reduced malaria cases but they are either costly, have poor efficacy or resistance has begun to develop. There is a global need for an effective pre-exposure prophylaxis treatment. The leading Malaria vaccine candidate is RTS,S which contains a monovalent Plasmodium falciparum circumsporozoite protein (CSP). The goal of this vaccine is to induce anti-CSP antibodies that would block sporozoite invasion of hepatocytes and thereby hinder parasite development into a blood-stage infection that causes malaria morbidity and mortality. Antibodies isolated from individuals who have received the RTS,S vaccine have been shown to prevent infection of hepatocytes, suggesting that CSP antibodies could be used prophylactically. However, phase III trial results of the vaccine have shown underwhelming efficacy in children. Growing resistance to transient protection strategies for travelers and low efficacy in vaccine trials suggest there is a need for a new treatment strategy. The generation of CSP specific human monoclonal antibodies (mAbs) would be useful as prevention especially for individuals that are temporarily exposed to Malaria in endemic regions such as travelers or military personnel. Isolation and production of therapeutic mAbs traditionally utilizes a handful of techniques including antibody engineering, phage display or hybridoma generation from transgenic mice. We have sorted antigen-specific memory B-cells from the peripheral blood of children naturally infected with malaria to isolate CSP-specific memory B-cells. These cells were individually sorted and PCR was performed to amplify antibody variable regions of the B-cell’s antibody mRNA. Samples that produced heavy and light chain antibody sequence were cloned and transiently expressed. We plan to characterize these mAbs for binding and neutralization of CSP to identify functional therapeutic mAbs

Maternal plasma DHA levels increase prior to 29 days post-LH surge in women undergoing frozen embryo transfer: a prospective, observational study of human pregnancy

Context: Docosahexaenoic acid (DHA) is an important fatty acid required for neurological development but its importance during early fetal neurological organogenesis is unknown. Objective: To assess plasma fatty acid changes in early pregnancy in women undergoing natural cycle-frozen embryo transfer as a means of achieving accurately-timed periconceptual sampling. Design: Women undergoing frozen embryo transfer were recruited and serial fasting blood samples were taken pre-luteinising hormone (LH) surge, and at days 18, 29 and 45 post-LH surge and fatty acids were analysed using gas chromatography. Setting: Assisted Conception Unit, Glasgow Royal Infirmary, Scotland Main outcome measures: Plasma fatty acid concentrations, influence of twin pregnancies on DHA plasma concentration. Results: In pregnant women, there was a rapid, early increase in the maternal rate of change of plasma DHA concentration observed by 29 days post-LH surge (mean±SD, from 0.1±1.3 to 1.6±2.9 nmol DHA per mL plasma per day). This early pressure to increase plasma DHA concentration was further emphasised in twin pregnancies where the increase in DHA concentration over 45 days was two-fold higher than in singleton pregnancies (mean±SD increase, 74±39 nmol/mL versus 36±40 nmol/mL). An index of delta-6 desaturase activity increased 30% and positively correlated with the rate of change of DHA concentration between day 18 and 29-post LH surge (R-squared adjusted = 41%, P=0.0002). DHA was the only fatty acid with a continual accelerated increase in plasma concentration and a positive incremental area under the curve (mean±SD, 632±911 nmol/mL x day) over the first 45 days of gestation. Conclusions: An increase in maternal plasma DHA concentration is initiated in human pregnancy prior to neural tube closure which occurs at 28 days' gestation

Challenges, Opportunities, and Adaptations of a College Preparatory 4-H Youth Development Program during COVID-19

SARS-CoV-2 (COVID-19) has proliferated across the United States, and in the process, it has disrupted all sense of normalcy. Because adolescents are in a critical period for growth and development, youth are particularly susceptible to negative impacts of disruption from COVID-19. Therefore, sustaining youth development programs is essential to ensuring positive youth development occurs despite significant challenges. Unfortunately, the implementation of programs that maintain safety precautions can be challenging. Many programs have been forced to either cancel all activities or to transition program elements to a virtual format. Rural Medical and Science Scholars (RMSS) program administrative staff made the decision to transition to a virtual delivery. Despite only having a few weeks to reshape the program, RMSS administrative staff were able to innovatively adapt to new challenges in order to deliver a successful program. The success of the program extends beyond its participants. By understanding potential program barriers and successful adaptation methods, other youth development programs will be better equipped to sustain program activities and youth outreach during the COVID-19 pandemic

Deciphering petrogenic processes using Pb isotope ratios from time-series samples at Bezymianny and Klyuchevskoy volcanoes, Central Kamchatka Depression

The Klyuchevskoy group of volcanoes in the Kamchatka arc erupts compositionally diverse magmas (high-Mg basalts to dacites) over small spatial scales. New high-precision Pb isotope data from modern juvenile (1956–present) erupted products and hosted enclaves and xenoliths from Bezymianny volcano reveal that Bezymianny and Klyuchevskoy volcanoes, separated by only 9 km, undergo varying degrees of crustal processing through independent crustal columns. Lead isotope compositions of Klyuchevskoy basalts–basaltic andesites are more radiogenic than Bezymianny andesites ([superscript 208]Pb/[superscript 204]Pb = 37.850–37.903, [superscript 207]Pb/[superscript 204]Pb = 15.468–15.480, and [superscript 206]Pb/[superscript 204]Pb = 18.249–18.278 at Bezymianny; [superscript 208]Pb/[superscript 204]Pb = 37.907–37.949, [superscript 207]Pb/[superscript 204]Pb = 15.478–15.487, and [superscript 206]Pb/[superscript 204]Pb = 18.289–18.305 at Klyuchevskoy). A mid-crustal xenolith with a crystallization pressure of 5.2 ± 0.6 kbars inferred from two-pyroxene geobarometry and basaltic andesite enclaves from Bezymianny record less radiogenic Pb isotope compositions than their host magmas. Hence, assimilation of such lithologies in the middle or lower crust can explain the Pb isotope data in Bezymianny andesites, although a component of magma mixing with less radiogenic mafic recharge magmas and possible mantle heterogeneity cannot be excluded. Lead isotope compositions for the Klyuchevskoy Group are less radiogenic than other arc segments (Karymsky—Eastern Volcanic Zone; Shiveluch—Northern Central Kamchatka Depression), which indicate increased lower-crustal assimilation beneath the Klyuchevskoy Group. Decadal timescale Pb isotope variations at Klyuchevskoy demonstrate rapid changes in the magnitude of assimilation at a volcanic center. Lead isotope data coupled with trace element data reflect the influence of crustal processes on magma compositions even in thin mafic volcanic arcs.University of Washington. Department of Earth and Space Science

The Littlest Higgs

We present an economical theory of natural electroweak symmetry breaking, generalizing an approach based on deconstruction. This theory is the smallest extension of the Standard Model to date that stabilizes the electroweak scale with a naturally light Higgs and weakly coupled new physics at TeV energies. The Higgs is one of a set of pseudo Goldstone bosons in an $SU(5)/SO(5)$ nonlinear sigma model. The symmetry breaking scale $f$ is around a TeV, with the cutoff \Lambda \lsim 4\pi f \sim 10 TeV. A single electroweak doublet, the ``little Higgs'', is automatically much lighter than the other pseudo Goldstone bosons. The quartic self-coupling for the little Higgs is generated by the gauge and Yukawa interactions with a natural size $O(g^2,\lambda_t^2)$, while the top Yukawa coupling generates a negative mass squared triggering electroweak symmetry breaking. Beneath the TeV scale the effective theory is simply the minimal Standard Model. The new particle content at TeV energies consists of one set of spin one bosons with the same quantum numbers as the electroweak gauge bosons, an electroweak singlet quark with charge 2/3, and an electroweak triplet scalar. One loop quadratically divergent corrections to the Higgs mass are cancelled by interactions with these additional particles.Comment: 15 pages. References added. Corrected typos in the discussion of the top Yukawa couplin

Anti-Müllerian hormone for the diagnosis and prediction of menopause:a systematic review

Background: The early onset of menopause is associated with increased risks of cardiovascular disease and osteoporosis. As a woman’s circulating anti-Müllerian hormone (AMH) concentration reflects the number of follicles remaining in the ovary and declines towards the menopause, serum AMH may be of value in the early diagnosis and prediction of age at menopause. Objective and Rationale: This systematic review was undertaken to determine whether there is evidence to support the use of AMH alone, or in conjunction with other markers, to diagnose menopause, to predict menopause, or to predict and/or diagnose premature ovarian insufficiency (POI). Search Methods: A systematic literature search for publications reporting on AMH in relation to menopause or POI was conducted in PubMed®, Embase®, and the Cochrane Central Register of Controlled Trials up to 31 May 2022. Data were extracted and synthesized using the Synthesis Without Meta-analysis for diagnosis of menopause, prediction of menopause, prediction of menopause with a single/repeat measurement of AMH, validation of prediction models, short-term prediction in perimenopausal women, and diagnosis and prediction of POI. Risk-of-bias was evaluated using the Tool to Assess Risk of Bias in Cohort Studies protocol and studies at high risk of bias were excluded. Outcomes: A total of 3207 studies were identified, and 41, including 28 858 women, were deemed relevant and included. Of the three studies that assessed AMH for the diagnosis of menopause, one showed that undetectable AMH had equivalent diagnostic accuracy to elevated FSH (>22.3 mIU/ml). No study assessed whether AMH could be used to shorten the 12 months of amenorrhoea required for a formal diagnosis of menopause. Studies assessing AMH with the onset of menopause (27 publications [n = 23 835 women]) generally indicated that lower age-specific AMH concentrations are associated with an earlier age at menopause. However, AMH alone could not be used to predict age at menopause with precision (with estimates and CIs ranging from 2 to 12 years for women aged <40 years). The predictive value of AMH increased with age, as the interval of prediction (time to menopause) shortened. There was evidence that undetectable, or extremely low AMH, may aid early diagnosis of POI in young women with a family history of POI, and women presenting with primary or secondary amenorrhoea (11 studies [n = 4537]). Wider Implications: The findings of this systematic review support the use of serum AMH to study the age of menopause in population studies. The increased sensitivity of current AMH assays provides improved accuracy for the prediction of imminent menopause, but diagnostic use for individual patients has not been rigorously examined. Prediction of age at menopause remains imprecise when it is not imminent, although the finding of very low AMH values in young women is both of clinical value in indicating an increased risk of developing POI and may facilitate timely diagnosis

Gauge/Anomaly Syzygy and Generalized Brane World Models of Supersymmetry Breaking

In theories in which SUSY is broken on a brane separated from the MSSM matter fields, supersymmetry breaking is naturally mediated in a variety of ways. Absent other light fields in the theory, gravity will mediate supersymmetry breaking through the conformal anomaly. If gauge fields propagate in the extra dimension they, too, can mediate supersymmetry breaking effects. The presence of gauge fields in the bulk motivates us to consider the effects of new messenger fields with holomorphic and non-holomorphic couplings to the supersymmetry breaking sector. These can lead to contributions to the soft masses of MSSM fields which dramatically alter the features of brane world scenarios of supersymmetry breaking. In particular, they can solve the negative slepton mass squared problem of anomaly mediation and change the predictions of gaugino mediation.Comment: 4 pages, RevTe

Horizontal, Anomalous U(1) Symmetry for the More Minimal Supersymmetric Standard Model

We construct explicit examples with a horizontal, ``anomalous'' $U(1)$ gauge group, which, in a supersymmetric extension of the standard model, reproduce qualitative features of the fermion spectrum and CKM matrix, and suppress FCNC and proton decay rates without the imposition of global symmetries. We review the motivation for such ``more'' minimal supersymmetric standard models and their predictions for the sparticle spectrum. There is a mass hierarchy in the scalar sector which is the inverse of the fermion mass hierarchy. We show in detail why DeltaS = 2 FCNC are suppressed when compared with naive estimates for nondegenerate squarks.Comment: Revised version clarifies calculation of FCNC amplitudes and rules out one model considered previousl