Molecular modeling.

<p><b>A</b>) Energy minimized structure of <b>4. B</b>) Surface diagram of PKCαC1B (blue) docked with <b>4</b> (magenta). <b>C</b>) Overlaid structures of αC1B (blue) and εC1B (magenta). Structure of <b>4</b> was minimized using Chem3D pro 12.0.2. Molecular docking was done using sybyl 8.0. The protein structures were visualized using UCSF chimera 1.6.1.</p

Effect of oleic acid, linoleic acid and linolenic acid on the membrane translocation of PKCα and PKCε.

<p>Upper panels, Western blot analysis of the cytosolic (C) and the membrane (M) fraction of (<b>A</b>) PKCα and (<b>B</b>) PKCε after the cells were treated with 10 µM of oleic acid, linoleic acid or linolenic acid for 24 h. Lower panel, bar graph of densitometry analysis of upper panel immunoblots (Mean± SE, n = 3). Control refers to the sample with no addition of compounds.</p

Effect of 2–5 on TPA-induced membrane translocation of PKCα and PKCε.

<p>Upper panels, Western blot analysis of the cytosolic (C) and membrane (M) fraction of (<b>A</b>) PKCα and (<b>B</b>) PKCε. Lower panel, bar graph of densitometry analysis of upper panel immunoblots (Mean ± SE, n = 3). Cells were treated with 100 µM of <b>2–5</b> and 100 nM of TPA for 1 h. Control refers to the sample with no addition of compounds.</p

Chemical structure of compounds 1–5.

<p>Chemical structure of compounds 1–5.</p

Absorption and fluorescence maxima of resveratrol (1) and its derivatives (2–5) in different solvent at 25°C.

<p>Absorption and fluorescence maxima of resveratrol (1) and its derivatives (2–5) in different solvent at 25°C.</p

Effect of 4 on the membrane translocation of PKCα and PKCε.

<p>Upper panels, Western blot analysis of the cytosolic (C) and the membrane (M) fractions of (<b>A</b>) PKCα and (<b>B</b>) PKCε after the cells were treated with varying concentration of <b>4</b> for 1 h. Lower panel, bar graph depicts the densitometry analysis of the upper panel immunoblots (Mean± SE, n = 3). Control refers to the sample with no addition of compounds.</p

Effect of 1 on the membrane translocation of PKCα and PKCε.

<p>Upper panels, Western blot analysis of the cytosolic (C) and the membrane (M) fractions of (<b>A</b>) PKCα and (<b>B</b>) PKCε after the cells were treated with varying concentration <b>1</b> for 1 h. Lower panel, bar graph of densitometry analysis of the upper panel immunoblots (Mean± SE, n = 3). Control refers to the sample with no addition of compounds.</p

Effect of solvent polarity on the absorption and fluorescence properties of 2 (5–10 µM).

<p>A), Normalized absorption and B) normalized florescence emission spectra of <b>2</b> in a) water, b) ethanol, c) acetonitrile and d) hexane.</p

Effect of 2–5 on the membrane translocation of PKCα and PKCε.

<p>Upper panels, Western blot analysis of the cytosolic (C) and the membrane (M) fractions of (<b>A</b>) PKCα and (<b>B</b>) PKCε after the cells were treated with 10 µM of <b>2, 3, 4</b> or <b>5</b> for 24 h. Lower panel, bar graph of densitometry analysis of the upper panel immunoblots (Mean± SE, n = 3). Control refers to the sample with no addition of compounds.</p

Effect of 1–5 on the expression of PKCα and PKCε.

<p>Upper panels, Western blot analysis of whole cell lysate of CHO-K1 cells after treatment with <b>1–5</b> (10 µM) for 24 h. Lower panel, bar graph of densitometry analysis of PKC expression (Mean ± SE, *P<0.05, n = 3). β actin was used as a reference for uniform loading. Control refers to the sample with no addition of compounds.</p