Distribution of GAD67 splice variants in rat brain at P1 (left panels) and P90 (right panels).

<p>GAD67 mRNA (A, B) and embryonic transcripts (EGAD; C–G) were detected using radioactive <i>in situ</i> hybridization. Autoradiographic film images of saggital sections showed a predominant expression of GAD67 mRNA in the olfactory bulb (OB) at both ages (A, B) and a moderate expression in the cerebellum at P90 (B). Intense labeling of embryonic messages was found in the subventricular zone (SVZ; C, E) and the olfactory bulb at P1 (C). The expression persisted until adulthood [D, F, G (enlarged SVZ from emulsion autoradiography)]. Sense probe for embryonic GAD67 mRNA was used as a negative control (H, I). Black spot in H displays an artefact. CBL – cerebellum; GCL – granule cell layer; HC – hippocampus; OB – olfactory bulb; PGL – periglomerular layer; RMS – rostral migratory stream; RT – reticular thalamic nucleus; S – striatum; SEL – subependymal layer; SVZ – subventricular zone. Scale bar A–F, I, H: 2 mm; G: 100 µm.</p

Sequences of gene specific primers and associated amplicon lengths and probes for <i>in situ</i> hybridization.

<p>Sequences of gene specific primers and associated amplicon lengths and probes for <i>in situ</i> hybridization.</p

Expression of embryonic splice variants in rat cortex (Ctx), hippocampus (HC), cerebellum (Cbl), and olfactory bulb (OB) at different postnatal ages.

<p>Probes were examined for embryonic GAD67 transcript expression by qPCR. Embryonic transcripts were jointly (EGAD) and separately (I-80 and I-86) amplified. Numbers of transcripts were calculated per 1000 transcripts of GAPDH. Data represent mean±SEM of five individual animals. *<i>P</i>≤0.05, the comparison of regions at each analyzed postnatal day (left table) and the comparison of ages within each region (right table) as analysed by one-way ANOVA followed by Tukey's post hoc test.</p

Expression of GAD65 and GAD67 in rat cortex (Ctx), hippocampus (HC), cerebellum (Cbl), and olfactory bulb (OB) at different postnatal ages.

<p>Probes were examined for GAD transcript expression by qPCR. Numbers of transcripts were calculated per 1000 transcripts of GAPDH. Data represent mean±SEM of five individual animals. ^#<i>P</i>≤0.05, compared with expression of GAD67 within the same region and at the same postnatal day and as evaluated by Mann-Whitney <i>U</i> test. *<i>P</i>≤0.05, the comparison of regions at each analyzed postnatal day (upper tables) and the comparison of ages within each region (lower tables) as analysed by one-way ANOVA followed by Tukey's post hoc test.</p