Comparison of the IHC markers CD138 and CD43 in oral leukoplakia: An original research

Background: In the clinical practice, one of the most common suspicious lesions that may be potentially malignant is oral leukoplakia. Globally, the rate at which it turns malignant varies. This study examines the levels of markers CD138 and 43 in oral leukoplakia. Materials and Methods: Twenty archival blocks of confirmed epithelial dysplasia were taken from the Department of Oral Pathology. These were processed for the identification of markers CD138 and 43 through Immuno Histo Chemistry (IHC). The blocks were divided equally for both the markers. Results: There was a noticeable difference in staining intensity between dysplastic tissue and nondysplastic epithelium. However, CD138 expression was low or weak in dysplastic epithelium. CD43 expression was negative in all nonhematopoietic tissues. Conclusion: Genes that are cancer associated have been found to have incredibly different impacts in numerous tissues during the multistep process of oral carcinogenesis. In tissues undergoing dysplastic changes, CD138 expression was shown to be decreased, which could point out the malignant changes initiated in the epithelium of the oral tissues

Do the supportive staff have enough oral health knowledge? - A study at a teaching health care institution in South India

Introduction: Oral hygiene plays an imperative role in preventing oral diseases. The assessment of knowledge, attitude and practice in relation to oral hygiene among the supportive staff members would help the professional, to inculcate in their patients the acceptable oral habits to thwart oral diseases as these staff members are a bridge between healthcare professional and the patient. Materials and Methods: A cross-sectional questionnaire-based study was conducted at a teaching health care institution in South India. The staff members who gave their consent after detailing the objective and rationale of the study were included in the study. Data were collected about their oral health knowledge, attitude and oral hygiene practices. Descriptive statistics was calculated. Results: Nearly 79% brushed their teeth only in the morning; 95% used a tooth brush and toothpaste regularly. Dental floss (0.5%) was the least used cleaning aid. About 57% of the participants visited the dentist only when necessary. The majority of the participants had no reason for not visiting the dentist. 72% were aware of the role of sugar in causing dental caries, and 55% were unaware of the role of fluoride in dental caries. Conclusion: This study demonstrates that the majority of them brush their teeth only once, and most of them used both tooth brush and tooth paste to brush their teeth. The participants visited dentist only in emergency, and their oral health knowledge is low

Characteristics of endodontic clinical trials registered in clinicalTrials.gov between 2000 and 2022

Aim: This study aims to examine the characteristics of endodontic clinical trials registered in the ClinicalTrials.gov database. Methods: The ClinicalTrials.gov website was searched for endodontic clinical trials that were registered between 2000 and 2022. The search term used was “endodontics.” As a result of the search strategy, 613 trials were identified. Out of which, 500 clinical trials met our inclusion criteria. For each clinical trial, information was extracted including the year of registration, location of the trial, type of trial, study design (allocation, intervention model, and masking) estimated enrollment, participants' age, health condition/problems studied, intervention/treatment, the status of the trial, study results, phase of the trial, and source of funding. Fisher's exact test was applied to identify the characteristics of clinical trials associated with funding. Results: The number of registered clinical trials gradually increased from 2000 to 2022. Out of 500 clinical trials, 456 were interventional, and 44 were observational. Only 18 clinical trials had secured funding. The majority of interventional trials (90.8%) are randomized, and the most common interventional model was parallel assignment (88.4%). It was observed that irreversible pulpitis was the most common condition studied (32.2%). Nonsurgical endodontic therapy was the most common intervention used in the trials (42.2%). Conclusion: The study provided an overview of endodontic clinical trials that were registered in the ClinicalTrials.gov. The findings showed that registered endodontic clinical trials have increased rapidly since 2000. The vast majority of trials took place in Africa. The majority of endodontic clinical trials followed rigorous methods in terms of allocation, intervention model, and masking. However, only a small portion of trials had secured funding. The findings also indicate the need for trial results to be made publicly available to enhance evidence-based practice in endodontics