1 research outputs found
Direct cell imprint lithography in superconductive carbon black polymer composites: process optimization, characterization and in vitro toxicity analysis
Cell imprint lithography (CIL) or cell replication plays a vital role in fields like biomimetic smart
culture substrates, bone tissue engineering, cell guiding, cell adhesion, tissue engineering, cell
microenvironments, tissue microenvironments, cell research, drug delivery, diagnostics, therapeutics
and many other applications. Herein we report a new formulation of superconductive carbon black
photopolymer composite and its characterization towards a CIL process technique. In this article, we
demonstrated an approach of using a carbon nanoparticle-polymer composite (CPC) for patterning
cells. It is observed that a 0.3 wt % load of carbon nanoparticles (CNPs) in a carbon polymer mixture
(CPM) was optimal for cell-imprint replica fabrication. The electrical resistance of the 3-CPC (0.3 wt %)
was reduced by 68% when compared to N-CPC (0 wt %). This method successfully replicated the
single cell with sub-organelle scale. The shape of microvesicles, grooves, pores, blebs or microvilli
on the cellular surface was patterned clearly. This technique delivers a free-standing cell feature
substrate. In vitro evaluation of the polymer demonstrated it as an ideal candidate for biomimetic
biomaterial applications. This approach also finds its application in study based on morphology,
especially for drug delivery applications and for investigations based on molecular pathways