Genetic variation explained by the 2Rb and 3Ra inversions.

<p>a) Genetic structure was assessed using genome-wide SNP data for individual <i>An</i>. <i>arabiensis</i> females using a PCA analysis. Three discrete PCA clusters were observed. Red = human-fed and blue = cattle-fed. There is an enrichment of cattle-fed individuals in the middle PCA cluster (P < 0.001; Fisher Exact). (b) To reveal differentiated genomic regions underlying the distinct PCA clusters (left, middle, and right) we plotted F_ST for each chromosome in 100kb windows with 20kb steps between the PCA clusters. The outside PCA clusters differed at the 2Rb and 3Ra inversions (orange), left versus middle PCA clusters differed at 2Rb only (green), and right versus middle differed at 3Ra only (black). Stars indicate the position of SNPs chosen for the inversion genotyping assay.</p

Relative host choice between villages.

<p>This figure describes the results of bloodmeal analysis of <i>An</i>. <i>arabiensis</i> collected from: Lupiro, Minepa, and Sagamaganga (SAGA). We detected multiple hosts in <5% of individuals. Different combinations of mixed host bloodmeals were pooled and shown as “Multiple”. A few chicken bloodmeals were also detected at each site.</p

3Ra and 2Rb Inversion frequencies by host.

<p>3Ra and 2Rb Inversion frequencies by host.</p