2 research outputs found
Bioremediation Potential of Cr(VI) by <i>Lysinibacillus cavernae</i> CR-2 Isolated from Chromite-Polluted Soil: A Promising Approach for Cr(VI) Detoxification
The present study focuses on an efficient Cr(VI)-reducing bacterial strain (CR-2) isolated from an abandoned chromate plant in Qinghai Province, China. CR-2 was confirmed as Lysinibacillus cavernae using 16S rRNA gene sequencing. CR-2 could survive at 500 mg L−1 Cr(VI) and effectively reduce Cr(VI) at concentrations of −1, a pH of 5–9, a temperature of 20–40 °C, and a salinity of 5–15 g L−1. According to the Box–Behnken experimental design, the maximum Cr(VI) removal efficiency by L. cavernae CR-2 was 76.21% under optimum conditions, which comprised a pH of 6.68, a temperature of 28.90 °C, and a salinity of 9.85 g L−1. With regard to Cr(VI) reduction mediated by L. cavernae CR-2, enhancement in efficiency was observed in the presence of Cu2+ and Ca2+, while significant inhibition in the reduction capacity occurred upon exposure to Mg2+, Ba2+, Ni2+, Pb2+, or Cd2+. Moreover, L. cavernae CR-2 tends to use glucose as an electron donor for the reduction of Cr(VI). Results of cell fraction separation and degeneration indicated that the Cr(VI) removal was primarily due to the reduction of Cr(VI) via chromium reductase in the cytoplasm. In addition, bioanalysis of L. cavernae CR-2 by SEM-EDS and TEM-EDS suggested that Cr was distributed both on the surface and in the cell cytoplasm. FT-IR analyses established that multiple functional groups (hydroxyl, carbonyl, amide, amino, and aldehyde groups) participated in the Cr(VI) biosorption on the cell surface. XPS and HPLC also showed that the Cr(III) end-products could be present as Cr(III) hydroxides or as organic–Cr(III) complexes. This study yields insights into the Cr(VI) bioreduction mechanism of L. cavernae CR-2.</p
A Recyclable Mineral Catalyst for Visible-Light-Driven Photocatalytic Inactivation of Bacteria: Natural Magnetic Sphalerite
Motivated
by recent studies that well-documented mineral photocatalyst
for bacterial inactivation, a novel natural magnetic sphalerite (NMS)
in lead–zinc deposit was first discovered and evaluated for
its visible-light-driven (VLD) photocatalytic bactericidal properties.
Superior to the reference natural sphalerite (NS), vibrating sampling
magnetometeric (VSM) analysis revealed the ferromagnetic property
of NMS, indicating its potential for easy separation after use. Under
the irradiation of fluorescence tubes, NMS could inactivate 7 log<sub>10</sub> Gram-negative <i>Escherichia coli</i> K-12 without
any regrowth and metal ions leached out from NMS show no toxicity
to cells. The cell destruction process starting from cell wall to
intracellular components was verified by TEM. Some products from damaged
cells such as aldehydes, ketones and carboxylic acids were identified
by FTIR with a decrease of cell wall functional groups. The relative
amounts of potassium ion leakage from damaged cells gradually increased
from initial 0 to approximately constant concentration of 1000 ppb
with increasing reaction time. Superoxide radical (•O<sub>2</sub><sup>–</sup>) rather than hydroxyl radical (•OH) was
proposed to be the primary reactive oxidative species (ROSs) responsible
for <i>E. coli</i> inactivation by use of probes and electron
spin resonance (ESR). H<sub>2</sub>O<sub>2</sub> determined by fluorescence
method is greatly involved in bacterial inactivation in both nonpartition
and partition system. Multiple cycle runs revealed excellent stability
of recycled NMS without any significant loss of activity. This study
provides a promising natural magnetic photocatalyst for large-scale
bacterial inactivation, as NMS is abundant, easily recycled and possessed
an excellent VLD bacterial inactivation ability