Estrogen and antiestrogen activities of identified substances in the YES and ER CALUX.

<p>CAS#… Chemical Abstracts Service Number.</p>a<p>… inhibition of human U2-OS osteosarcoma cell growth at higher concentrations.</p>b<p>… inhibition of yeast growth at higher concentrations.</p

Androgen and antiandrogen activities of identified substances in the YAS and AR CALUX.

<p>CAS#… Chemical Abstracts Service Number.</p>a<p>… inhibition of human U2-OS osteosarcoma cell growth at higher concentrations.</p>b<p>… inhibition of yeast growth at higher concentrations.</p

Antiestrogenic activity of samples spiked with 8/l 17β-estradiol standard as measured by ERα CALUX.

<p>Antiestrogenic activity of samples spiked with 8/l 17β-estradiol standard as measured by ERα CALUX.</p

Validation parameters of GC-MS (SIM) analysis of 29 reference substances.

<p>Validation parameters of GC-MS (SIM) analysis of 29 reference substances.</p

Antiandrogenic activity of samples spiked with 2.4/l DHT standard as measured by the YAS.

<p>Antiandrogenic activity of samples spiked with 2.4/l DHT standard as measured by the YAS.</p

Food simulants for the migration of packaging samples.

a<p>Sample was previously tested by Kirchnawy et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100952#pone.0100952-Kirchnawy1" target="_blank">[1]</a> using the YES.</p>b<p>Migration was done at 60°C for 10 days.</p

Androgen and antiandrogen activity of migrates from plastic samples.

a<p>… inhibition of response to DHT in undiluted samples.</p>b<p>… inhibition of yeast growth in undiluted samples.</p><p>−… no antiandrogen activity was detected.</p><p>+… antiandrogen activity in the range of the activity of 0.01 to 0,1 mg/l flutamide.</p><p>++… antiandrogen activity in the range of the activity of 0.1 to 1 mg/l flutamide.</p><p>+++… antiandrogen activity in the range of the activity of 1 to 10 mg/l flutamide.</p

Identification of substances in migrates of samples which were hormone active in bioassay analysis.

a<p>… identification of the substance verified by comparison to a standard.</p>b<p>… identification of the substances by database comparison of mass spectra, not verified by comparison to a standard.</p><p>LOD…Limit of detection.</p><p>LOQ…Limit of quantification.</p><p>CAS#… Chemical Abstracts Service Number.</p

Estrogen and antiestrogen activity of migrates from plastic samples.

a<p>… inhibition of response to 17β-estradiol in undiluted samples.</p>b<p>… inhibition of yeast growth in undiluted samples.</p><p>−… no antiestrogen activity was detected.</p><p>+… antiestrogen activity in the range of the activity of 0.01 to 0.1 mg/l 4-OHT.</p><p>++… antiestrogen activity in the range of the activity of 0.1 to 1 mg/l 4-OHT.</p><p>+++… antiestrogen activity in the range of the activity of 1 to 10 mg/l 4-OHT.</p

GC-MS-Analysis: Total Ion Chromatogram (TIC) of a migrate of sample CF 1.

<p>GC-MS-Analysis: Total Ion Chromatogram (TIC) of a migrate of sample CF 1.</p