Chromatograms of Permeated MANT-Iota-Carrageenan Samples After 0, 1, 2 and 3 Hours (pH 7.5).

<p>The concentrations of the fluorescence marker MANT was determined by HPLC in order to derive amounts of iota-carrageenan that have permeated bovine mucosa after 0 hour (blue), 1 hour (purple), 2 hours (pink) and 3 hours (green) incubation with MANT-iota-carrageenan, at pH 7.5. A retention time of approx. 7 minutes was defined for MANT-iota-carrageenan (highlighted by black box) and approx. 15 minutes for free MANT.</p