An algorithm for the selection of route dependent orientation information

Landmarks are important features of spatial cognition and are naturally included in human route descriptions. In the past algorithms were developed to select the most salient landmarks at decision points and automatically incorporate them in route instructions. Moreover, it was shown that human route descriptions contain a significant amount of orientation information, which support the users to orient themselves regarding known environmental information, and it was shown that orientation information support the acquisition of survey knowledge. Thus, there is a need to extend the landmarks selection to automatically select orientation information. In this work, we present an algorithm for the computational selection of route dependent orientation information, which extends previous algorithms and includes a salience calculation of orientation information for any location along the route. We implemented the algorithm and demonstrate the functionality based on OpenStreetMap data

Spatial Knowledge and Urban Planning (Editorial)

Urban planning is simultaneously shaped by and creates new (spatial) knowledge. The changes in planning culture that have taken place in the last decades - especially the so-called communicative turn in planning in the 1990s - have brought about an increased attention to a growing range of stakeholders of urban development, their interests, logics, and participation in planning as well as the negotiation processes between these stakeholders. However, while this has also been researched in breadth and depth, only scant attention has been paid to the knowledge (claims) of these stakeholders. In planning practice, knowledge, implicit and explicit, has been a highly relevant topic for quite some time: It is discussed how local knowledge can inform urban planning, how experimental knowledge on urban development can be generated in living labs, and what infrastructures can process "big data" and make it usable for planning, to name a few examples. With the thematic issue on "Spatial Knowledge and Urban Planning" we invited articles aiming at exploring the diverse understandings of (spatial) knowledge, and how knowledge influences planning and how planning itself constitutes processes of knowledge generation. The editorial gives a brief introduction to the general topic. Subsequently, abstracts of all articles illustrate what contents the issue has to offer and the specific contribution of each text is carved out. In the conclusion, common and recurring themes as well as remaining gaps and open questions at the interface of spatial knowledge and urban planning are discussed

Subcellular localization of the antidepressant-sensitive norepinephrine transporter

<p>Abstract</p> <p>Background</p> <p>Reuptake of synaptic norepinephrine (NE) via the antidepressant-sensitive NE transporter (NET) supports efficient noradrenergic signaling and presynaptic NE homeostasis. Limited, and somewhat contradictory, information currently describes the axonal transport and localization of NET in neurons.</p> <p>Results</p> <p>We elucidate NET localization in brain and superior cervical ganglion (SCG) neurons, aided by a new NET monoclonal antibody, subcellular immunoisolation techniques and quantitative immunofluorescence approaches. We present evidence that axonal NET extensively colocalizes with syntaxin 1A, and to a limited degree with SCAMP2 and synaptophysin. Intracellular NET in SCG axons and boutons also quantitatively segregates from the vesicular monoamine transporter 2 (VMAT2), findings corroborated by organelle isolation studies. At the surface of SCG boutons, NET resides in both lipid raft and non-lipid raft subdomains and colocalizes with syntaxin 1A.</p> <p>Conclusion</p> <p>Our findings support the hypothesis that SCG NET is segregated prior to transport from the cell body from proteins comprising large dense core vesicles. Once localized to presynaptic boutons, NET does not recycle via VMAT2-positive, small dense core vesicles. Finally, once NET reaches presynaptic plasma membranes, the transporter localizes to syntaxin 1A-rich plasma membrane domains, with a portion found in cholera toxin-demarcated lipid rafts. Our findings indicate that activity-dependent insertion of NET into the SCG plasma membrane derives from vesicles distinct from those that deliver NE. Moreover, NET is localized in presynaptic membranes in a manner that can take advantage of regulatory processes targeting lipid raft subdomains.</p

The inotropic agent digitoxin strengthens desmosomal adhesion in cardiac myocytes in an ERK1/2-dependent manner

Desmosomal proteins are components of the intercalated disc and mediate cardiac myocyte adhesion. Enhancement of cardiac myocyte cohesion, referred to as \textquotedblpositive adhesiotropy\textquotedbl, was demonstrated to be a function of sympathetic signaling and to be relevant for a sufficient inotropic response. We used the inotropic agent digitoxin to investigate the link between inotropy and adhesiotropy. In contrast to wild-type hearts, digitoxin failed to enhance pulse pressure in perfused mice hearts lacking the desmosomal protein plakoglobin which was paralleled with abrogation of plaque thickening indicating that positive inotropic response requires intact desmosomal adhesion. Atomic force microscopy revealed that digitoxin increased the binding force of the adhesion molecule desmoglein-2 at cell-cell contact areas. This was paralleled by enhanced cardiac myocyte cohesion in both HL-1 cardiac myocytes and murine cardiac slices as determined by dissociation assays as well as by accumulation of desmosomal proteins at cell-cell contact areas. However, total protein levels or cytoskeletal anchorage were not affected. siRNA-mediated depletion of desmosomal proteins abrogated increase of cell cohesion demonstrating that intact desmosomal adhesion is required for positive adhesiotropy. Mechanistically, digitoxin caused activation of ERK1/2. In line with this, inhibition of ERK1/2 signaling abrogated the effects of digitoxin on cell-cell adhesion and desmosomal reorganization. These results show that the positive inotropic agent digitoxin enhances cardiac myocyte cohesion with reorganization of desmosomal proteins in an ERK1/2-dependent manner. Desmosomal adhesion seems to be important for a sufficient positive inotropic response of digitoxin treatment, which can be of medical relevance for the treatment of heart failure

The Lantern Vol. 47, No. 2, May 1981

• Festival • Ode to Old Tom • Living Room • Writing a Poem • Mission Impossible • The Hinge is Oiled • The Potter\u27s Field at Malvern • Points of Time • Attempted Autonomy • My Love • Love, not War • Death Comes Quickly • You Can\u27t Always Get What You Don\u27t Really Want • You See (Johnny\u27s Tale): An Elegy • Sanguine Hopeshttps://digitalcommons.ursinus.edu/lantern/1118/thumbnail.jp