Gel shift assay for TIMP3 SNPs rs9862 and rs11547635.

<p>A) Probe names and sequences. rs9862 and rs11547635 are indicated with arrows. The 12 bp palindromic sequence is highlighted in bold and core ETS1 consensus sites are underlined. Probes are named according to alleles at sites rs9862 and rs11547635, respectively. Only one strand of the double-stranded probes is shown. B) Results. Probe and competitor names correspond to the sequences in A. Lane 15 is a control, with no nuclear extract. Potential protein complexes bound to the probes are indicated with letters on the left side of the image. Complex I appears to be specific to probes with the rs9862 C allele, whereas complexes II and IV are specific to probes with the rs9862 T allele. Complex III binds irrespective of rs9862 allele, and is competed off by unlabelled ETS1.</p

Structure of the <i>TIMP3</i> gene.

<p>Coding regions of exons are shown as dark bars; 5′ and 3′ untranslated regions are shown as lighter bars; introns and flanking sequence are illustrated by a line. The diagram is not to scale. Thick lines below the gene structure indicate the regions sequenced. SNPs associated with survival are indicated by vertical lines. Variants detected by sequencing are shown as circles. An LD plot based on study data is below the gene. Numbers in the plot are r² values; boxes with darker shading illustrate higher LD; lighter shading represents weaker LD. Circled SNPs show significant association with survival.</p

Hazard ratio (HR) and 95% Confidence intervals (CI) estimated for the association between <i>TIMP3</i> gene variations and survival of the study cohort.

*<p>Adjusted for patient age, disease stage, surgery, chemotherapy, radiation therapy, location of tumor.</p

Survival of the study cohort by <i>TIMP3</i> variations.

<p>Kaplan-Meier survival curves and log-rank test p-values are shown. <b>A</b>) rs130274, <b>B</b>) rs1962223, <b>C</b>) rs5754312, <b>D</b>) rs715572.</p

Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges - Fig 6

<p><b>(A)</b> Differences in the proportions of monocytes, T cells and NK cells with age. <b>(B)</b> Differences with age in the % of T cells that are CD4+ and CD8+, in the CD4/CD8 ratio with age, in the proportion of T cells that are Tregs, and in the proportion of CD4 and CD8 cells that are CD28+ and -. <b>(C)</b> Anti-CD3 + anti-CD28 stimulated proliferation (left panel) and IFNγ production (right panel). Results are expressed as the mean ± SEM. * indicates a statistically significant difference (P <0.05) between the young and old cohorts.</p

Demographic and clinical features of the cohort by survival status.

<p>Demographic and clinical features of the cohort by survival status.</p

A comparison of <i>E</i>. <i>coli</i> stimulated levels of inflammatory markers from young and old volunteers collected with EDTA versus heparin.

<p>● = males 20–35 years old; ■ = females 20–35 years old; ▲ = males >50 years old and ▼ = females >50 years old. Results are expressed as the mean ± SEM. * indicates a statistically significant difference (P <0.05) between the young and old cohorts. ^# indicates a statistically significant difference (P<0.05) between male and female cohorts.</p

Differences in endogenous levels of IL-17, G-CSF, IL-17, MIP1α and PGE₂ levels at different ages.

<p>Blood collected in EDTA tubes from 20 healthy men + 20 healthy women, aged 20–34 (●), 35–49 (■) and 50–77 (▲) years old and assayed for G-CSF, IL-17, MIP1α and PGE₂, using Luminex beads and, for PGE₂ levels, an EIA. Results are expressed as the mean ± SEM. * indicates statistically significant differences (P <0.05) between the different cohorts.</p

A comparison of HSV-1 stimulated levels of inflammatory markers from young and old volunteers collected with EDTA versus heparin.

<p>● = males 20–35 years old; ■ = females 20–35 years old; ▲ = males >50 years old and ▼ = females >50 years old. Results are expressed as the mean ± SEM. * indicates a statistically significant difference (P <0.05) between the young and old cohorts.</p

A comparison of endogenous levels of inflammatory markers in fresh blood samples from young and old volunteers collected with EDTA versus heparin.

<p><b>(A)</b> Luminex results. ● = males 20–35 years old; ■ = females 20–35 years old; ▲ = males >50 years old and ▼ = females >50 years old. <b>(B)</b> CRP, PGE₂, and total TGFβ levels in zero time (endogenous) samples. For the top 2 panels, ● = males 20–35 years old; ■ = females 20–35 years old; ▲ = males >50 years old and ▼ = females >50 years old. ^Δ denotes a significant (P<0.05) difference in cytokine/chemokine levels between EDTA and heparin samples. Results are expressed as the mean ± SEM. * indicates a statistically significant difference (P <0.05) between the young and old cohorts.</p