8 research outputs found
A model of IN:LEDGF:viral DNA based on the PFV IN structure.
<p>(<b>A</b>) A model of HIV-1 IN complex with 4 IN subunits, 4 LEDGF subunits (light gray), and two viral DNA strands (dark gray); (<b>A, B</b>) Left panels (IN NTD = green, CCD = blue, CTD = red. (<b>A, B</b>) Right panels show functional sites (green = DNA binding), red = dimerization interface in 1EX4 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Chen1" target="_blank">[10]</a>, cyan = dimerization interface in 1WJA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Cai1" target="_blank">[11]</a>; blue = tetramerization interface in 1K6Y <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Wang1" target="_blank">[8]</a>; purple = zinc binding site <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Cai1" target="_blank">[11]</a>; brown = reverse transcriptase binding site <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Wilkinson1" target="_blank">[31]</a>, light brown = tetramerization residues <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Lutzke2" target="_blank">[16]</a>. (<b>B</b>) A 90° rotation about the Y-axis of <b>A</b>. Orange arrowhead indicated channel proposed to bind target DNA <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Hare1" target="_blank">[36]</a>.</p
Sources of data in the HIVToolbox MySQL database.
<p>*Sequence features that are multimerization interfaces were calculated in Molmol based on residues that were less than 3.25 Ã… away from at least one residue in another subunit <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone.0020122-Koradi1" target="_blank">[63]</a>.</p
Interactive protein display page for Tat in HIVToolbox.
<p>Sequence window, Structure windows, Log windows, and Sequence Alignment section of HIVToolbox are shown. The interactive results page for HIV-1 tat is shown. The scrollable sequence window shows the protein sequence, domains (with colored fonts), functional residues (highlighted), protein-protein interaction sites (thin lines under sequence), mapped protein structures (thin colored lines over sequence) and minimotifs (figures under sequence). The synchronized interactive structural displays show domains and selected minimotifs (left panel), functional sites and selected protein-protein interaction sites (center panel), and residues conserved at or above a sequence conservation threshold selected with a slider or text box (right panel). The Sequence Alignment section shows alignment of a representative set of 20 sequences with the RefSeq sequence and the structure sequence. A second tab reveals a position specific-scoring matrix of amino acid frequencies at each position in the protein. More details about the features and use of HIVToolbox are in the supplement, and video tutorials are at Bio-Toolkit [<a href="http://www.bio-toolkit.com" target="_blank">http://www.bio-toolkit.com</a>].</p
Diagram of software architecture of HIVToolbox.
<p>Diagram of software architecture of HIVToolbox.</p
Analysis of Integrase model tetramers and hetero-octamers.
<p>Output of HIVToolbox showing surface plots of IN structural models. (<b>A, B</b>) IN tetramers showing domain organization (left panels) and locations of actives site residues (royal blue), proposed viral DNA binding grooves (yellow lines), proposed genomic DNA binding channel (red line), and zinc binding sites (cyan). Yellow numbers indicate the subunit too which the domain belongs. (<b>C</b>) IN:LEDGF hetero-octamers models showing organization of proteins (left panel) and proposed DNA binding groove (middle panel, red line). LEDGF subunits are colored grey. (<b>D</b>) An end-on view of the proposed host DNA binding channel in the IN:LEGDF hetero-octamer model shown in (<b>C</b>) (red circle).</p
Sequence conservation of CK2 sites in different strains of HIV-1.
<p>*‘del’ indicate that the residue is deleted or was not present in one or more structures.</p
Statistics for data in the HIVToolbox database.
<p>Statistics for data in the HIVToolbox database.</p
Analysis of Integrase with HIVToolbox.
<p>(<b>A–C</b>) Output of HIVToolbox showing relationships of IN elements shown in a model constructed from superposition of the catalytic domains in structures 1EX4 and 1K6Y. Residues 1–7, 47–55, 140–148, and 270–288 are unstructured and not shown. The left panels shows domains [NTD (red), CCD (Blue), and CTD (green)] and minimotifs; the center panels show functional sites and protein-protein interactions the right panels shows residues that are >98% conserved in 3787 HIV-1 IN isolates (yellow). (<b>A</b>) Location of three of the four putative CK2 phosphorylation sites located on the surface of the IN CCD (left panel); the 4<sup>th</sup> CK2 site is in the CTD unstructured region. Numbers indicate the positions of putative phosphorylation sites. D270 is the last residue in the structure (orange). Conservation of the residues on CK2 sites is shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020122#pone-0020122-t001" target="_blank"><b>Table 1</b></a>. (<b>B</b>) Conservation and location of the dimerization interface(s). Residues at the dimerization interface less than 3.25 Å from atoms in the other chain are colored: (red, 1EX4), (cyan, 1WJA), and (lighter cyan, 1K6Y). (<b>B, C</b>) Conservation and location of protein-protein interaction sites, modification sites, and DNA binding sites. (<b>C</b>) is a 180° rotation of (<b>B</b>) about the z-axis. (<b>A, B, C</b>) Sites are colored: DNA binding = green, Importin 7 binding = dark purple and dark green, Zn binding = purple, Karyopherin α5 binding = teal and orange, LEDGF binding = teal, Lysine acetylation = dark green, proline isomerization = orange, active site = royal blue, reverse transcriptase (RT) binding = brown.</p